Abstract
Although the immuno-modulatory and stress-relieving properties of β-glucan is well elucidated in humans and other animal models, including fish, its role as a dietary supplement on reproduction is extremely scarce. Therefore, in this study, adult female fish were fed one of four test diets having 0 (control), 0.5, 1, and 1.5% β-d-glucan for 130 days and its effect on reproductive performance, ovarian and liver histology, sex hormones, and transcript abundance of selected reproduction-related genes was assessed. Low dietary intake of β-glucan improved fertilization and hatching rates (p<0.05). The relative fecundity and percentage of spawning females were higher (non-significant) in 0.5% β-glucan-fed groups. Surprisingly, even after 130 days, spawning did not occur in 1.5% β-glucan-fed individuals. Irrespective of β-glucan intake, all the brooders recorded similar plasma 17β-estradiol and maturation-inducing hormone (p>0.05). Higher intake of β-glucan (1.5%) upregulated aromatase genes without a parallel increase in 17β-estradiol. However, plasma vitellogenin increased with increasing β-glucan up to 1.0% then declined at 1.5% (p<0.05). The fish that received control, 0.5, and 1.5% β-glucan recorded similar vitellogenin levels in their plasma. Significantly higher plasma cortisol was evidenced in 1.5% β-glucan fed brooders (p<0.05). Histologically, higher follicular atresia and leaking of yolk material was evidenced in 1.5% β-glucan-fed group. Liver histology revealed the highest nutrient/lipid accumulation in fish that received 1.0% and 1.5% β-glucan. This study demonstrated the stimulatory effect of β-glucan intake at a lower dose (0.5%) on reproduction. However, higher intake (1.5%) could perturb normal reproductive function in a fish model and caused an increased number of atretic follicles leading to spawning/reproductive failure.
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Acknowledgements
The authors duly acknowledge the Director, ICAR-DCFR, for facilitating all the logistics support during the study. The sincere help extended by Sh. Harish Chandra and Sh. Prem Pandey for rearing and maintenance of the experimental fish is greatly acknowledged.
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The study was financially supported by the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India (vide reference no. BT/PR26920/AAQ/3/884/2017).
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Alexander Ciji: conceptualization; experiment execution; data analysis; manuscript’s original draft preparation; M.S. Akhtar: conceptualization; fund acquisition; experiment execution; formal analysis; manuscript review and editing; Priyanka H. Tripathi: gene expression analyses; Maneesh Kumar Dubey: histological sample processing; Prakash Sharma: histological data interpretations; manuscript reviewing and editing.
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This research complies with current animal welfare laws in India, and the animals were used in accordance with the CPCSEA ((Committee for the Purpose of Control and Supervision of Experiments on Animals), Ministry of Environment & Forests (Animal Welfare Division), Govt. of India) guidelines on animal care and use in scientific research. The study was carried out with the approval of competent authorities of the ICAR-Directorate of Coldwater Fisheries Research at Bhimtal, Uttarakhand, India.
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Ciji, A., Akhtar, M.S., Tripathi, P.H. et al. Higher intake of β-glucan impairs reproduction in a female teleost, Tor putitora (Hamilton, 1822). Fish Physiol Biochem 50, 589–603 (2024). https://doi.org/10.1007/s10695-023-01292-4
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DOI: https://doi.org/10.1007/s10695-023-01292-4