Abstract
The aim of this study was to investigate the effect of dietary vitamin A on juvenile Chinese perch (Siniperca chuatsi). Chinese perch were fed with five experimental diets containing 0, 20, 40, 60, and 80 mg VA·kg−1 for 8 weeks. Results showed that dietary vitamin A significantly influenced the fish's growth, feed utilization, glucose and lipid metabolism, appetite, and antioxidant capacity. Vitamin A-supplemented groups had higher weight gain rate (WGR) and specific growth rate (SGR) compared to the control group. Feed conversion ratio (FCR) was also lower in the vitamin A-supplemented groups. Dietary vitamin A had no significant effect on the survival rate (SR). Compared to the control group, fish fed with vitamin A had increased feed intake (FI), and the expression of appetite-promoting genes (npy and agrp) was significantly higher in the 40 mg VA·kg−1 group. Vitamin A also enhanced the utilization of dietary protein by Chinese perch. The serum glucose content of the fish fed with 40 mg VA·kg−1 diet was significantly higher than that of the control group and 20 mg VA·kg−1 diet, indicating that the promoting effect of VA on gluconeogenesis was greater than that on glycolysis. Additionally, dietary vitamin A increased the expression of lipid metabolism-related genes (hl and fas) and antioxidant genes (nrf2 and gpx) in the fish. These results suggest that the optimal vitamin A requirement of juvenile Chinese perch bream was estimated to be 37.32 mg VA·kg−1 based on broken-line regression analysis of WGR. In conclusion, this study provides valuable insights into the potential benefits of dietary vitamin A on the growth, metabolism, and antioxidant capacity of Chinese perch.
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Acknowledgements
The research would like to thank the Innovation Base for Chinese Perch Breeding, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, China, for providing the breeding site. Thanks to the others who contributed to the experiment.
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The Key Research & Development Program of Hubei Province(2022BBA0051); Key Research and Development Project of Hubei Province (2020BBA035).
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Xu-Fang Liang: experimental design; Junliang Chen: carrying out experiments, collecting samples and analyzing data; Ming Zeng: writing and revising manuscripts. Di Peng, Ruipeng Xie and Dongliang Wu: participated in sample collection.
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Chen, J., Zeng, M., Liang, XF. et al. Dietary supplementation of VA enhances growth, feed utilization, glucose and lipid metabolism, appetite, and antioxidant capacity of Chinese perch (Siniperca chuatsi). Fish Physiol Biochem 50, 225–237 (2024). https://doi.org/10.1007/s10695-023-01221-5
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DOI: https://doi.org/10.1007/s10695-023-01221-5