Abstract
The aim of this study was to investigate the survival rate, biochemical indices, and metabolome changes of the large yellow croaker after 48 h of live transportation. Two hundred and forty large yellow croakers (body weight: 23.4 ± 5.3 g, total length: 12.2 ± 0.7 cm) were used in this experiment. The transport buckets were filled with fresh seawater and the parameters of the water were a temperature of 16 ± 0.5 °C and a dissolved oxygen content of 6.0–7.2 mg/L. Large yellow crokers were first divided to 0, 10, 20, and 30 mg/L MS-222 groups to observe the 12 h survival rate. The survival rate of 10 mg/L MS-222 group (T1) was the 95%, highest of all, and was further analyzed. The results of liver biochemical indices indicated inhibition of gluconeogenesis and pentose phosphate pathway metabolism. In addition, metabolomics analysis identified significantly differentially expressed metabolites between T1 group and 0 mg/L MS-222 control (C) groups. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) results revealed that the pathways of amino acid metabolism, especially the lysine, aspartate, and homoserine in the liver were significantly affected. In conclusion, the combination of metabolomics and liver biochemical assays provided a characterization of the response mechanism of L. crocea exposed to live transportation.
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Data availability
The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.
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We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.
Funding
This study was financially supported by the National Key Research and Development Project (2019YFD0900901), the project of science and technology of Zhoushan (2019C21016, 2021C31003), 2020 Second Municipal Modern Fisheries Development Project, Document No. 21 of the Zhoushan Treasury Bureau (2020), Cooperative extension plan of major agricultural technologies in Zhejiang province (2020XTTGSC04).
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Yang Gao conceived the project; Yangchen Zhou conducted the project, wrote and revised the manuscript; Zhangjie Chu, Xiaolong Yin, and Weiye Li participated in the discussions and provided valuable advice.
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All experimental and sampling procedures were conducted according to the Guidelines of the Animal Care of the Zhejiang Ocean University and were approved by the Ethics Committee of the university.
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Zhou, Y., Yin, X., Li, W. et al. Effects of transportation on physiological indices and metabolomics of the large yellow croaker Larimichthys crocea. Fish Physiol Biochem 49, 641–654 (2023). https://doi.org/10.1007/s10695-023-01216-2
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DOI: https://doi.org/10.1007/s10695-023-01216-2