Abstract
The purpose of this research is to explore the interaction between dietary leucine and isoleucine levels on whole-body composition, plasma and liver biochemical indexes, amino acids deposition in the liver, and amino acid metabolism of blunt snout bream (Megalobrama amblycephala). The test fish (average weight: 56.00 ± 0.55 g) were fed one of six diets at random containing two leucine levels (1.70% and 2.50%) and three isoleucine levels (1.00%, 1.20%, and 1.40%) for 8 weeks. The results showed that the final weight and weight gain rate were the highest in the fish fed low-level leucine and high-level isoleucine diets (P > 0.05). Furthermore, the crude lipid content was significantly adjusted by diets with diverse levels of leucine and isoleucine (P < 0.05). In addition, interactive effects of these two branched-chain amino acids (BCAAs) were found on plasma total protein, blood ammonia, and blood urea nitrogen of test fish (P < 0.05). Additionally, the liver amino acid profiles were significantly influenced by the interactive effects of the two BCAAs (P < 0.05). Moreover, interactive effects of dietary leucine and isoleucine were significantly observed in the expressions of amino acid metabolism-related genes (P < 0.05). These findings suggested that dietary leucine and isoleucine had interaction. Meanwhile, the interaction between them was more conducive to the growth and quality improvement of blunt snout bream when the dietary leucine level was 1.70% and isoleucine level was 1.40%.
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This research was supported by the Development Plan Project in Key Areas of Guangdong Province (Project No: 2020B0202010001), the Jiangsu Natural Science Foundation for basic research (BK20201325), and the earmarked fund for CARS (CARS-45–12).
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Mang-mang Wang, Guang-zhen Jiang, and Wen-bin Liu provided ideas and schemes for this experiment; Mang-mang Wang and Yang-yang Huang completed the feeding trial; Mang-mang Wang, Yi-lin Zhang, and Jing-Wei Fan assisted sampling. Mang-mang Wang finished data analysis and manuscript writing; Kang Xiao, Xi Wang, Hui-xing Guo, Xiang-fei Li, and Guang-zhen Jiang polished the manuscript.
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Highlights
• Interaction between leucine and isoleucine affects the bioavailability of leucine and isoleucine in blunt snout bream.
• The interaction between leucine and isoleucine will affect their ratio in feed.
• The best combinations of leucine and isoleucine in dietary of blunt snout bream would be LL-HI.
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Wang, Mm., Huang, Yy., Liu, Wb. et al. Interactive effects of dietary leucine and isoleucine affect amino acid profile and metabolism through AKT/TOR signaling pathways in blunt snout bream (Megalobrama amblycephala). Fish Physiol Biochem 50, 385–401 (2024). https://doi.org/10.1007/s10695-022-01161-6
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DOI: https://doi.org/10.1007/s10695-022-01161-6