Abstract
Among the wide variety of alternative ingredients aimed to substitute fish meal in aquafeeds, insect meals have been recently proposed as novel, nutritionally good dietary components. In the present study, five isoproteic and isoenergetic experimental diets formulated with varying dietary levels of Madagascar cockroach meal substituting fishmeal on a dietary protein basis (0, 25, 50, 75, and 100%) were supplied to.
Nile tilapia juveniles (Oreochromis niloticus) for 29 days. Production parameters were compared among treatments, and the relative assimilation of the dietary nitrogen supplied by fishmeal and insect meal was estimated. To this end, nitrogen stable isotope analyses were applied to diets and fish muscle tissue. The isotopic changes elicited by the experimental ingredients were used to estimate the time required to reach isotopic equilibrium, the nitrogen turnover rates, and nitrogen residency times in muscle tissue. The latter were short and ranged from 4.7 to 6.2 d, except in diet containing 100% cockroach meal (7.8 d). No statistical differences were detected in final mean weight, specific growth, and survival rates among treatments. The relative proportions of dietary nitrogen and total dry matter supplied by insect and fish meal to growth were similar to the established dietary proportions after these were corrected for their respective apparent digestibility coefficients. The cockroach meal present in diets containing both main ingredients supplied relatively high proportions of dietary nitrogen (from 16 to 69%) to the biosynthesis of fish muscle tissue.
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Funding
The present study was financially supported by the Faculty of Agronomy of the Autonomous University of Nuevo Leon (UANL), through the “Programa de Apoyo y Fortalecimiento a la Investigación 2022.”
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All authors contributed to the study conceptualization, review, and editing of the manuscript. ODGP obtained the biological material and acquired the main experimental ingredients for the nutritional study and also participated in the diet’s formulation, biometric processes, data integration, sample collection, and pre-analysis of samples and wrote first draft of the manuscript. CAGM obtained the biological material and acquired the main experimental ingredients for the nutritional study. RMSC obtained the biological material and acquired the main experimental ingredients for the nutritional study. DAVC participated in the diet’s formulation, biometric processes, data integration, sample collection, and pre-analysis of samples. JGD interpreted the isotopic data and ran the mixing models. GMD assisted with funding acquisition, laboratory resources, and experimental setup and wrote first draft of the manuscript. All authors commented on previous versions of the manuscript. All authors have read and approved the final manuscript.
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Ethical animal management approval for fish is not required by Mexican regulations. However, the feeding trial was developed adhering to maintenance and euthanasia protocols recommended by Sloman et al. (2019).
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García-Pérez, O.D., Sanchez-Casas, R.M., Moreno-Degollado, G. et al. Substitution of fish meal with Madagascar cockroach (Gromphadorhina portentosa) meal in diets for juvenile Nile tilapia (Oreochromis niloticus): effects on growth, nutrient assimilation, and nitrogen turnover rates. Fish Physiol Biochem 48, 1587–1597 (2022). https://doi.org/10.1007/s10695-022-01153-6
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DOI: https://doi.org/10.1007/s10695-022-01153-6