Abstract
This study aimed to investigate the effects of transport stress on the physiological responses and immunity of Megalobrama amblycephala (blunt snout bream). Fish (109.67 ± 1.51 g) were sampled at nine time points: before transport (control), at 0 h, 1 h, 3 h, 6 h, 12 h, 24 h, 3 days, and 7 days after 4 h of medium-distance transportation, and four fish were sampled in each time point. The results showed that plasma cortisol, triiodothyronine (T3), complement component 3 (C3), complement component 4 (C4), immunoglobulin M (IgM) and nitrogen monoxide (NO) concentrations, and alternative complement pathway (ACH50), acid phosphatase (ACP), and myeloperoxidase (MPO) activities all reached the peak at 0 h after transportation; C4 and NO concentrations as well as ACP and MPO activities returned to the control level after 1 h, ACH50 activity as well as cortisol, T3, and IgM concentration returned to the control level after 12 h, and C3 concentration returned to the control level after 24 h respectively. Plasma glucose and total protein concentrations as well as lysozyme activity all reached the peak at 1 h after transportation, total protein concentration and lysozyme activity returned to the control level after 3 h, and glucose concentration returned to the control level after 6 h (P < 0.05). Liver heat shock protein 70 expression reached the peak at 1 h after transportation, and returned to the control level after 24 h; liver heat shock protein 90 expression reached the peak at 0 h after transportation and returned to the control level after 12 h (P < 0.05). Overall, these findings suggested that 4 h of medium-distance transportation caused stress response of blunt snout bream, and transport stress had a significant effect on plasma indicators. But the recovery of 24 h after transport could return the physiological response, immune indexes, and the expression of heat shock protein to the normal level. This also provided data support for the medium-distance transportation of blunt snout bream in the future.
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This work was supported by National Modern Agriculture Industry Technology System (CARS-45–45), National Freshwater Aquatic Germplasm Resource Bank (2021DKA30470), Basic Research Business Fund Project of Henan Province (JBKY2021), and Modern Agricultural Industry Technology System of Henan Province (S2014-10).
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Funding acquisition and project administration: Qin Zhang; Writing-original draft preparation data curation and data validation: Bingke Wang; Methodology and writing—reviewing and editing: Yanhui Wang and Tao Jia; Resources: Jianxin Feng and Changyi Qu; Software: Xiaojun Wu and Xingli Yang. All authors read and approved the final manuscript.
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Wang, B., Wang, Y., Jia, T. et al. Changes in physiological responses and immunity of blunt snout bream Megalobrama amblycephala from transport stress. Fish Physiol Biochem 48, 1183–1192 (2022). https://doi.org/10.1007/s10695-022-01108-x
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DOI: https://doi.org/10.1007/s10695-022-01108-x