Abstract
This study evaluated the effects of three levels of loading density (200, 300 and 400 g L−1) and four levels of duration (1, 3, 6 and 9 h) on the mortality, plasma cortisol and chloride levels and growth of rohu Labeo rohita fingerlings in a transport simulation. While total immediate mortality was low (4–12%), delayed mortality was high (27–49%). Initial capture and handling were found to constitute the most stressful phase as indicated by elevated plasma cortisol. The levels of cortisol continued to rise at 6 and 9 h after transport, with the highest level observed at 9 h after transport. The level of plasma chloride fell after transport across all density levels and treatment durations. Specific growth rate (SGR, %/day) and total bacterial density (cfu/g) were not different between and within treatments, and between treatments and the controls. This study demonstrates that transport has a profound effect on the stress responses of rohu fingerlings. Although the level of immediate mortality was low, the higher level of delayed mortality has serious implications in terms of production inefficiency for grow-out farmers.
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References
Adams SM (1990) Status and use of biological indicators for evaluating the effects of stress on fish. In: Adams SM (ed) Biological indicators of stress in fish. Am Fish Soc Symp 8:1–9
Barton B (2002) Stress in fishes: a diversity with particular reference in changes in circulating corticosteroids. Int Comp Biol 42:517–525
Barton BA, Peter RE (1982) Plasma cortisol stress response in fingerling rainbow trout, Salmo gairdneri Richardson, to various transport conditions, anesthesia, and cold shock. J Fish Biol 20:39–51
Carmichael GJ (1984) Long distance transport of intensively reared largemouth bass. The Prog Fish-Cult 46:111–115
Carmichael GJ, Tomasso JR, Simco BA, Davis KB (1984) Characterization and alleviation associated with hauling large mouth bass. Trans Am Fish Soc 113:778–785
Chatterjee N., Pal AS, Das T, Mohammed MS, Sarma K, Venkateswarlu G, Mukherjee SC (2006) Secondary stress responses in Indian Major carps Labeo rohita (Hamilton), Catla catla (Hamilton), Cirrhinus mrigala (Hamilton) fry to increasing packing densities. Aquacult Res 37:472–476
Dey MM, Paraguas FJ, Bhatta R., Alam F., Weimin M., Piumsombun S, Koeshandrajana S, Dung LTC, Sang NV (2005) Carp production in Asia: past trends and present status in Asia. In: Penman DJ, Gupta MV, Dey MM (eds) Carp genetic resources for aquaculture in Asia. World Fish Center Technical Report 65. Penang, Malaysia, pp 6–15
Gomes LC, Araujo-Lima CARM, Roubach R, Chippari-Gomes AR, Lopes NP, Urbinati EC (2003) Effect of fish density during transportation on stress and mortality of juvenile tambaqui Colossoma macropomum. J World Aquacult Soc 34:76–84
Gregory TR, Wood CM (1999) The effects of chronic plasma cortisol elevation on the feeding behavior, growth, competitive ability, and swimming performance of juvenile rainbow trout. Physiol Biochem Zool 72:286–295
Hasan M, Bart AN (2007) Improved survival of rohu, Labeo rohita (Hamilton-Buchanan) and silver carp, Hypophthalmichthys molitrix (Valenciennes) fingerlings using low-dose quinaldine and benzocaine during transport. Aquacult Res 38:50–58
Hasan M, Bart AN (2006) Carp seed traders in Bangladesh: sources of livelihoods and vulnerability resulting from fish seed mortality. Asia-Pacific J Rural Dev 16 (in press)
Hopkins KD (1992) Reporting fish growth: a review of the basics. J World Aquacult Soc 23:173–179
Johnson DL, Metcalf MT (1982) Causes and controls of freshwater drum mortality during transportation. Trans Am Fish Soc 111:58–62
Lewis DJ, Wood GD, Gregory R (1996) Trading the silver seed. University Press Limited. Dhaka-1000, Bangladesh
Mazeaud MM, Mazeaud F., Donaldson EM (1977) Primary and secondary effects of stress in fish: some new data with a general review. Trans Am Fish Soc 106:201–212
Mazik PM, Simco BA, Parker NC (1991) Influence of water hardness and salts on survival and physiological characteristics of stripped bass during transport. Trans Am Fish Soc 120:121–126
McCormick SD, Shrimpton JM, Carey JB, O’Dea MF, Sloan KE, Moiyama S, Bjornsson B Th (1998) Repeated acute stress reduces growth rate of Atlantic salmon parr and alters plasma levels of growth hormone, insulin-like growth factor 1 and cortisol. Aquaculture 168:221–235
McDonald G, Milligan L (1997) Ionic, osmotic and acid-base regulation in stress. In: Iwama GK, Pickering AD, Sumpter JP, Schreck, CB (eds) Fish stress and health in Aquaculture, vol 62. Society for experimental biology seminar series. Cambridge University Press, Cambridge, pp 119–144
Pankhurst NW, Van Der Kraak G (1997) Effects of stress on reproduction and growth of fish. In: Iwama, GK, Pickering, AD, Sumpter, JP, Schreck, CB (eds) Fish stress and health in Aquaculture, vol 62. Society for experimental biology seminar series. Cambridge University Press, Cambridge, pp 73–94
Pickering AD (1993) Growth and stress in fish. Aquaculture 111:51–63
Pickering AD, Stewart A (1984) Acclimation of the interregnal tissue of the brown trout, Salmo trutta L., to chronic crowing stress. J Fish Biol 24:731–740
Specker JL, Schreck CB (1980) Stress responses to transportation and fitness for marine survival in coho salmon (Oncorhynchus kisutch) smolts. Can J Fish Aquat Sci 37:765–769
Sumpter JP (1997) The endocrinology of stress. In: Iwama, GK, Pickering, AD, Sumpter, JP, Schreck, CB (eds) Fish stress and health in aquaculture, vol 62. Society for experimental biology seminar series. Cambridge University Press, Cambridge, pp 145–170
Urbinati EC, de Abreu JS, de Silva Camargo AC, Parra MAL (2004) Loading and transport stress of juvenile matrinxa (Brycon cephalus Characidae) at various densities. Aquaculture 229:389–400
Wahab MA, Rahman MM, Milstein A (2002) The effect of common carp, Cyprinus carpio (L.) and mrigal, Cirrhinus mrigala (Hamilton) as bottom feeders in major Indian carp polycultures. Aquacult Res 33:547–556
Wedemeyer GA (1997) Effects of rearing conditions on the health and physiological quality of fish in intensive culture. In: Iwama, GK, Pickering, AD, Sumpter JP, Schreck CB (eds) fish stress and health in aquaculture, vol 62. Society for experimental biology seminar series. Cambridge University Press, Cambridge, pp 35–71
Acknowledgements
This study was funded by the Support for University Fisheries Education and Research (SUFER) Project, DFID Bangladesh. The authors are also grateful to Jagorany Chakra, Jessore, Bangladesh for providing the experimental ponds for the delayed mortality and growth trials. We thank Professor Peter Edwards for his careful review and constructive suggestions, both of which improved the manuscript.
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Hasan, M., Bart, A.N. Effects of capture, loading density and transport stress on the mortality, physiological responses, bacterial density and growth of rohu Labeo rohita fingerlings. Fish Physiol Biochem 33, 241–248 (2007). https://doi.org/10.1007/s10695-007-9136-7
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DOI: https://doi.org/10.1007/s10695-007-9136-7