Abstract
Lysine is one of the most important essential amino acids in fish, especially in the feed formulated with high levels of plant ingredients. Lysine restriction always led to growth inhibition and poor feed utilization. However, little information was available on its effects on digestion, absorption, and metabolism response in fish. In the present study, three experimental diets were formulated with three lysine levels, 1.69% (LL group), 3.32% (ML group), and 4.90% (HL group). A 10-week feeding trial was carried out to explore the effects of dietary lysine levels on the digestive enzymes, amino acid transporters, and hepatic intermediary metabolism in turbot (Scophthalmus maximus). As the results showed, the activities of lipase and trypsin in ML group were higher than in other groups. Lysine restriction inhibited the expression levels of peptides and amino acid transporters such as PpeT1, y+LAT2, b0,+AT, and rBAT but significantly induced the expression of CAT1. Meanwhile, lysine deficiency elevated the content of T-CHO and LDL-C in plasma, while a higher HDL-C/LDL-C ratio was observed in ML group. For hepatic intermediary metabolism, the increase of lysine level induced the mRNA expression of G6Pase1 and FBPase, but no differences were observed in the expression of the key regulators in glycolysis pathway, such as GK and PK. Furthermore, an appropriate increase in the level of lysine promoted the genes involved in lipolysis, including PPARα, ACOX1, CPT1A, and LPL. However, no differences were observed in the expression of PPARγ, FAS, SREBP1, and LXR, which were important genes related to lipid synthesis. These results provide clues on the metabolic responses on dietary lysine in teleost.
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This study was supported by the National Key R&D Program of China (2018YFD0900400), Key R&D Program in Shandong Province (2020ZLYS03), and China Agriculture Research System (CARS-47-G10).
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The authors’ responsibilities were as follows: X. W., G. H., and K. M. designed research; X. H. and X. S. conducted research and analyzed data; H. Z. and C. L. provided essential reagents and materials. X. H. and X. W. wrote the original manuscript. All authors read and approved the final manuscript.
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Huang, X., Song, X., Wang, X. et al. Dietary lysine level affects digestive enzyme, amino acid transport and hepatic intermediary metabolism in turbot (Scophthalmus maximus). Fish Physiol Biochem 48, 1091–1103 (2022). https://doi.org/10.1007/s10695-022-01098-w
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DOI: https://doi.org/10.1007/s10695-022-01098-w