Abstract
Nanoselenium (nano-Se) shows unique protective effects against environmental heat stress in rainbow trout as a selenium source additive and free radical scavenger. Accordingly, we investigated the effects of supplementation with different levels of nano-Se (0, 5, and 10 mg/kg) and before and after heat stress (24°C) for different treatment times on the dynamic changes of rainbow trout liver tissue structure, lipid changes, biochemical properties, and gene expression. The results showed that, under heat stress, the fish supplementation of 5 mg/kg nano-Se significantly increased liver glutathione peroxidase (GPx) activity and upregulated expression levels of HSP70b, HSP90a1, GPx1a, and Trx mRNAs, while liver alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD), and malondialdehyde (MDA) levels as well as tissue structure damage and lipid accumulation were decreased. Combining the trends for the above indicators indicated that stress began to increase significantly at 8 h. It can be concluded that supplementation with 5 mg/kg nano-Se effectively alleviates stress damage in rainbow trout. Furthermore, stress at 24°C for 8 h can be thought of as a critical time point for the study of heat stress in rainbow trout, with significant changes in response but no serious damage. Thus, these results provide a reference for the addition of nano-Se to rainbow trout feed and provide theoretical and practical guidance for enhancing the resistance of rainbow trout to heat stress.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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This work was funded by the National Natural Science Foundation of China (Grant No. 31960727).
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LL and ZL conceived and designed the experiments. LL and JL performed the experiments. LL and JQ analyzed the data. GZ and JS contributed reagents, materials, and tools and collected the samples. LL and ZL wrote the manuscript and revised the manuscript. All authors read and approved the final manuscript.
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Fig. S1
Detection results for total RNA extraction from liver using 1.0% agarose gel electrophoresis. (PNG 1316 kb)
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Li, L., Liu, Z., Quan, J. et al. Dietary nanoselenium supplementation for heat-stressed rainbow trout: effects on organizational structure, lipid changes, and biochemical parameters as well as heat-shock-protein- and selenoprotein-related gene expression. Fish Physiol Biochem 48, 707–722 (2022). https://doi.org/10.1007/s10695-022-01084-2
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DOI: https://doi.org/10.1007/s10695-022-01084-2