Abstract
Excessive lipid deposition in farmed fish is a challenge in the aquaculture industry. To study the effect of dietary calcium pyruvate (CaP) on lipid accumulation in fish, we used a high fat diet (HFD) to establish a lipid accumulation model in juvenile golden pompano (Trachinotus ovatus) and supplemented with 0%, 0.25%, 0.50%, 0.75% and 1.0% CaP (diets D0–D4, respectively). After 8-week feeding in floating cages, dietary CaP significantly improved growth performance, which peaked in fish fed diet D3. Supplementation of CaP significantly decreased whole body lipid content in fish fed D2–D4 and hepatosomatic index and liver lipid content in fish fed D3 and D4. Serum and hepatic antioxidant indices, including glutathione, catalase and superoxide dismutase, showed generally increasing trends in fish fed diets with CaP. In addition, increasing dietary CaP increasingly reduced hepatic activities of hexokinase, phosphofructokinase and pyruvate kinase involved in glycolysis, and increased glycogen contents of the liver and muscle. Dietary CaP up-regulated the liver mRNA expression of pparα, cpt1, hsl and fabp1, but down-regulated expression of srebp-1, fas and acc. In conclusion, 0.75% CaP improved growth performance and reduced excessive lipid deposition by affecting fatty acid synthesis and lipolysis in juvenile T. ovatus fed HFD.
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This research was supported by the Guangdong Provincial Key R&D Programme (2021B0202050001), Natural Science Foundation of Guangdong Province (2020A1515011252), Innovation and Strong School Projects in Guangdong Province (2020ZDZXlO14, 2018KCXTD012) and Guangdong Agriculture Research System (2022KJ150).
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Cuihong You, Yiru Shao and Shuqi Wang contributed to the study conception and design. Material preparations were performed by Zhiyou Xie, Shusheng Liang and Yucong Hong. Data collection was performed by Cuiying Chen, Dizhi Xie and Yuanyou Li. Analysis was performed by Yiru Shao, Zhiyong Xie and Cuiying Chen. The first draft of the manuscript was written by Yiru Shao and Douglas R. Tocher, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Shao, Y., Xie, Z., Liang, S. et al. Dietary calcium pyruvate could improve growth performance and reduce excessive lipid deposition in juvenile golden pompano (Trachinotus ovatus) fed a high fat diet. Fish Physiol Biochem 48, 555–570 (2022). https://doi.org/10.1007/s10695-022-01077-1
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DOI: https://doi.org/10.1007/s10695-022-01077-1