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Molecular cloning, characterization and expression analysis of P53 from high latitude fish Phoxinus lagowskii and its response to hypoxia

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Abstract

As an intermediate link between multiple cellular stresses and cellular responses, p53, together with its upstream and downstream regulators and related genes, constitutes a complex network that regulates cellular stresses and cellular responses. However, no studies have investigated p53 in Phoxinus lagowskii, particularly the expression of p53 under different hypoxic conditions. In the present study, the cDNA of p53 from the Phoxinus lagowskii was cloned by the combination of homology cloning and rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of Pl-p53 was 1878 bp, including an open reading frame (ORF) of 1116 bp encoding a polypeptide of 371 amino acids with a predicted molecular weight of 41.22 kDa and a theoretical isoelectric point of 7.38. Quantitative real-time (qRT) PCR assays revealed that Pl-p53 was commonly expressed in all tissues examined, with highest expression in the heart. In addition, we investigated the expression of Pl-p53 in different tissues under different hypoxic conditions. In the short-term hypoxia group, Pl-p53 expression was down-regulated in both the brain and heart. The Pl-p53 expression was significantly elevated at 6 h in the muscle and liver, and was significantly up-regulated at 24 h in spleen. These results suggest that Pl-p53 plays different regulatory roles and provide a theoretical basis for the changes of p53 in fish facing hypoxic environments.

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All datasets generated for this study are included in the article.

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Funding

The research was supported by the National Natural Science Foundation of China (32170523), Harbin Normal University Graduate Innovation Project (HSDSSCX2021-08), Outstanding Youth Science Fund Project of Harbin Normal University and the funding of “Blue granary” scientific and technological innovation of China (2019YFD0900405), fundamental scientific research business expenses of colleges and universities in Heilongjiang Province (2021-KYYWF-0165).

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Authors and Affiliations

  1. Key Laboratory of Biodiversity of Aquatic Organisms, College of Life Science and Technology, Harbin Normal University, Harbin, 150025, China

    Jing Wang, Xi Chen, Xinrui Ge, Zhen Wang & Weijie Mu

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  1. Jing Wang
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  2. Xi Chen
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  3. Xinrui Ge
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  4. Zhen Wang
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  5. Weijie Mu
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JW and WM designed the experiments, analyzed data and wrote the manuscript together. The samples were obtained, and the experiments were carried out by ZW, XC and XG.

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Correspondence to Weijie Mu.

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Wang, J., Chen, X., Ge, X. et al. Molecular cloning, characterization and expression analysis of P53 from high latitude fish Phoxinus lagowskii and its response to hypoxia. Fish Physiol Biochem 48, 631–644 (2022). https://doi.org/10.1007/s10695-022-01072-6

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