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PKA/ATGL signaling pathway is involved in ER stress-mediated lipolysis in adipocytes of grass carp (Ctenopharyngodon idella)

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Abstract

The relationship between endoplasmic reticulum stress (ER stress) and lipolysis in mammals has been widely studied, but it is relatively scarce in fish. The present study used grass carp Ctenopharyngodon idella as a model to investigate the effect of ER stress on lipolysis in adipocytes of fish. We found that ER stress evoked by tunicamycin (TM) treatment significantly induced lipolysis in adipocytes. Subsequently, in order to further investigate whether protein kinase A (PKA) is involved in ER stress-induced lipolysis, we treated adipocytes with PKA activator forskolin and inhibitor H89. The results showed that the mechanism was related to the activation of PKA, especially the catalytic subunit PRKACBa. Notably, we also found that PKA regulates lipolysis by targeting mRNA level and protein and enzyme activities of adipotriglyceride lipase (ATGL). Taken together, our findings suggest that PKA/ATGL signaling pathway is involved in ER stress-mediated lipolysis of grass carp adipocytes. It provides a theoretical basis for further study on the mechanism of lipolysis in fish and other vertebrates.

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The datasets and materials used in this study are available from the corresponding author on reasonable request.

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Funding

The study was financially supported by the National Natural Science Foundation of China (NSFC) (31772863, 32072989, and 32002403).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest Agriculture and Forestry University, Yangling, 712100, China

    Shanghong Ji, Jian Sun, Chenchen Bian, Xiaocheng Huang & Hong Ji

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  1. Shanghong Ji
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  2. Jian Sun
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  3. Chenchen Bian
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  4. Xiaocheng Huang
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  5. Hong Ji
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Contributions

Shanghong Ji: contributed to the conception and designed of the study, wrote original draft. Jian Sun: data curation and revised the manuscript. Chenchen Bian: investigation and revised the manuscript. Xiaocheng Huang: methodology and revised the manuscript. Hong Ji: project administration and revised the manuscript. All authors contributed to interpreting the results, and read and approved the final manuscript.

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Correspondence to Hong Ji.

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We promised that all procedures of the experiment were performed in accordance with the Guide for Care and Use of Laboratory Animals and approved by the Northwest A&F University Institutional Animal Care and Use Committee.

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This study was supported by College of Animal Science and Technology, Northwest Agriculture and Forestry University. The authors disclose no conflicts of interest. All authors listed have approved to submit to your journal.

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Ji, S., Sun, J., Bian, C. et al. PKA/ATGL signaling pathway is involved in ER stress-mediated lipolysis in adipocytes of grass carp (Ctenopharyngodon idella). Fish Physiol Biochem 48, 683–691 (2022). https://doi.org/10.1007/s10695-021-01032-6

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