Abstract
During salinity stress, osmoregulatory processes in euryhaline fish need to modify for their survival, and glucose is the preferred mode of extra energy during such conditions. These organisms must have a proper mechanism to maintain glucose homeostasis during such modified osmoregulatory process across different body fluids. Hence, we studied high salinity effect on regulation of glucose homeostasis in Mozambique tilapia. The fish were induced to 15‰ salinity for 21 days. Glucose, glycogen, ion concentrations, Na+-K+-ATPase, pyruvate kinase, γ-amylase activities and GLUT mRNA expressions were investigated in liver, intestine, gill and white muscle tissues. At the end of experiment, Na+ ion concentrations, glucose content and activity of Na+-K+-ATPase especially in the gill and intestine were increased, while decrease in liver and gill glycogen content was seen. Lower concentration of glycogen decrease was observed in the intestine and white muscle of the treated group. High pyruvate kinase activity was noticed in liver and gill tissues that correlates with high Na+-K+-ATPase activity. Elevated γ-amylase activity was observed in the liver and intestine suggesting breakdown of glycogen; however, gill and white muscle did not show any increased activity. Increase in GLUT1 and GLUT4 mRNA expressions was observed especially in the gill and intestine, while increase in GLUT2 mRNA expressions was observed in the liver. Upregulations of GLUTs suggest higher influx of glucose into the cell for catabolism to provide energy and further to drive the enhanced osmoregulatory process. These findings suggest glucose homeostasis being regulated in Mozambique tilapia during salinity acclimation.
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The authors wish to thank the Department of Zoology, Goa University, for the support in completion of the project.
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The project was undertaken with funds provided by the Department of Zoology, Goa University.
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Complete performing of experiment and data analysis was done by Prateek Angadi except for mRNA expression data which was performed and analysed by Moitreyi Das. The idea, design of project, and guidance were done by Ramaballav Roy. All authors have equally contributed in revising the manuscript.
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Angadi, P., Das, M. & Roy, R. Effect of high salinity acclimation on glucose homeostasis in Mozambique tilapia (Oreochromis mossambicus). Fish Physiol Biochem 47, 2055–2065 (2021). https://doi.org/10.1007/s10695-021-01022-8
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DOI: https://doi.org/10.1007/s10695-021-01022-8