Abstract
Rainbow trout (Oncorhynchus mykiss) farming is one of the major aquacultures in Turkey. Some conditions in fish farming can induce oxidative stress leading to the deterioration in properties such as appearance/color, texture, and flavor in fish meat. This situation may cause the consumer not to prefer edible fish. Although there are some studies on the impacts of light intensity on fish welfare, the changes in the antioxidant enzyme activities have not been elucidated. In the current study, it was intended to examine in rainbow trout how cultivating under different wavelengths affects the antioxidant enzymes and acetylcholine esterase (AChE) activity, because its activity is associated with oxidative stress, and also the determination of which light is suitable for fish welfare was aimed. Rainbow trout larvae were grown under four lights with different wavelengths: natural sunlight and incandescent long-wave (red light), medium-wave (green light), and short-wave (blue light) LED light. The experiment lasted for 64 days. Biochemical assays were carried on in the brain, gill, and liver of rainbow trout. Antioxidant enzymes and AChE activity, which play an important role in the central nervous system, were assayed. In gill tissues, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glucose 6 phosphate dehydrogenase (G6PD), glutathione reductase (GR), glutathione S-transferase (GST), and AChE activities increased under all three light wavelengths. In the liver, while activities of antioxidant enzymes and AChE decreased in red light, all of them increased in blue and green light. In the brain, GPx, GST, G6PD, and SOD activities were reduced but AChE activity did not alter under all three light sources. In conclusion, light sources with different spectral structures caused important changes in the activities of antioxidant enzymes in rainbow trout. On this basis, it may be thought that this may be a response to the changing redox status of a cell. Based on our results, blue light sources may be suggested for fish welfare in rainbow trout culture, and providing fish welfare by changing light sources can be easy and cheap in fish farming.
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This work was supported by the Van Yüzüncü Yıl University, Scientific Research Projects Department as project number 2013-FBE-YL001.
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Conceived and designed the experiments: UG and ŞÖ. Performed the experiments: UG, ŞÖ, MA, and BK. Analyzed the data: UG, ÖİK, and MY. Contributed reagents/materials/analysis tools: ÖİK and MY. Wrote the paper: UG and ŞÖ. All authors read and approved the final manuscript.
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The study was carried out following ethical approval by the University of Van YYU, Van, Turkey. All animal care and procedures were in accordance with the ethical principles of animal use and care.
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This study was presented as a poster abstract in “The 41st FEBS Congress, s.405, Kuşadası/ Turkey, 2016” (which was canceled).
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Güller, U., Önalan, Ş., Arabacı, M. et al. Effects of different LED light spectra on rainbow trout (Oncorhynchus mykiss): in vivo evaluation of the antioxidant status. Fish Physiol Biochem 46, 2169–2180 (2020). https://doi.org/10.1007/s10695-020-00865-x
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DOI: https://doi.org/10.1007/s10695-020-00865-x