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The dynamic process of dietary soybean β-conglycinin in digestion, absorption, and metabolism among different intestinal segments in grass carp (Ctenopharyngodon idella)

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Abstract

The present study aimed to investigate the dynamic process of soybean β-conglycinin in digestion, absorption, and metabolism in the intestine of grass carp (Ctenopharyngodon idella). Fish fed with 80 g β-conglycinin/kg diet for 7 weeks, the intestinal digestive enzyme was extracted to hydrolyze β-conglycinin in vitro, the free amino acid and its metabolism product contents in intestinal segments were analyzed. The present study first found that β-conglycinin cannot be thoroughly digested by fish intestine digestive enzyme and produces new products (about 60- and 55-kDa polypeptides). The indigestible β-conglycinin further caused the free amino acid imbalance, especially caused free essential amino acid deficiency in the proximal intestine but excess in the distal intestine. Moreover, these results might be partly associated with the effect of β-conglycinin in amino acid transporters and tight junction-regulated paracellular pathway. Finally, dietary β-conglycinin increased the content of amino acid catabolism by-product ammonia while decreased the amino acid anabolism product carnosine content in the proximal intestine and distal intestine. Thus, the current study first and systemically explored the dynamic process of β-conglycinin in digestion, absorption, and metabolism, which further supported our previous study that dietary β-conglycinin suppressed fish growth and caused intestine injure.

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Acknowledgments

The authors would like to thank the personnel of the teams below for their kind assistance.

Funding

This research was financially supported by the National Natural Science Foundation of China (31672662), the National Key R&D Program of China (2018YFD0900400), the Earmarked Fund for China Agriculture Research System (CARS-45), the Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture), the Foundation of Sichuan Youth Science and Technology Innovation Research Team (2017TD0002), the Key Research and Development Plan in Sichuan Province (2018NZ0007), and the Sichuan Science and Technology Program (2019YFN0036), and supported by the 111 project (D17015).

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Authors and Affiliations

  1. Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, 611130, China

    Xu-Dong Duan, Lin Feng, Wei-Dan Jiang, Pei Wu, Yang Liu, Jun Jiang & Xiao-Qiu Zhou

  2. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China

    Lin Feng, Wei-Dan Jiang, Pei Wu, Yang Liu & Xiao-Qiu Zhou

  3. Key laboratory of Animal Disease-resistant Nutrition, Chengdu, 611130, Sichuan Province, China

    Lin Feng & Xiao-Qiu Zhou

  4. Key laboratory of Animal Disease-resistant Nutrition, Ministry of Education, Chengdu, 611130, China

    Wei-Dan Jiang & Pei Wu

  5. Key laboratory of Animal Disease-resistant Nutrition and Feed, Ministry of Agriculture and Rural Affairs, Chengdu, 611130, China

    Yang Liu

  6. Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, China

    Bei-Ping Tan & Qi-Hui Yang

  7. Animal Nutrition Institute, Sichuan Academy of Animal Science, Chengdu, 610066, China

    Sheng-Yao Kuang & Ling Tang

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  1. Xu-Dong Duan
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Correspondence to Xiao-Qiu Zhou.

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Duan, XD., Feng, L., Jiang, WD. et al. The dynamic process of dietary soybean β-conglycinin in digestion, absorption, and metabolism among different intestinal segments in grass carp (Ctenopharyngodon idella). Fish Physiol Biochem 46, 1361–1374 (2020). https://doi.org/10.1007/s10695-020-00794-9

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