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Supplementation of enzyme-treated soy protein saves dietary protein and promotes digestive and absorptive ability referring to TOR signaling in juvenile fish

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Abstract

This study was conducted to evaluate the effect of enzyme-treated soy protein (ETSP) supplementation in the low-protein diet on growth performance, digestive and absorptive capacities, and related signaling molecules’ gene expressions in juvenile Jian carp. The results showed that percent weight gain (PWG), specific growth rate (SGR), and feed intake (FI) were decreased by reducing dietary protein from 34 to 32% (P < 0.05). Supplying low-protein diet with optimal ETSP increased previously mentioned indices of juvenile Jian carp (P < 0.05), which also had no significant difference with the high-protein diet (34%CP) (P > 0.05). Compared with the low-protein diet, appropriate ETSP supplementation in the low-protein diet increased (P < 0.05) (1) the trypsin, lipase, and amylase activities in the hepatopancreas; (2) cholecystokinin concentration in the proximal intestine; (3) the γ-glutamyl transpeptidase (γ-GT), alkaline phosphatase (AKP), and Na+/K+-ATPase activities in all intestinal segments; and (4) the messenger RNA (mRNA) levels of trypsin, lipase, and amylase in hepatopancreas and γ-GT in the mid (MI) and distal (DI) intestine, alkaline phosphatase in MI, and Na+/K+-ATPase and target of rapamycin in all intestinal segments. At the same time, appropriate ETSP supplementation in the low-protein diet downregulated the mRNA levels of AKP in the DI and eIF4E-binding protein 2 in all intestinal segments (P < 0.05). In conclusion, adding 10 g ETSP/kg diet in the low-protein diet can restore the growth performance and digestive and absorptive abilities to the levels in group with 34% dietary protein. Supplementation of optimal ETSP in the low-protein diet enhanced the digestive and absorptive abilities and regulated the signaling molecules related to the TOR signaling pathway.

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Abbreviations

AKP:

Alkaline phosphatase

CK:

Creatine kinase

DI:

Distal intestine

ETSP:

Enzyme-treated soy protein

FBW:

Final body weight

FE:

Feed efficiency

FI:

Feed intake

γ-GT:

γ-Glutamyl transpeptidase

HIS:

Hepatosomatic index

HPC:

Hepatopancreas protein content

HW:

Hepatopancreas weight

IBW:

Initial body weight

IL:

Intestinal length

IPC:

Intestinal protein content

ISI:

Intestosomatic index

IW:

Intestinal weight

MI:

Mid intestine

PER:

Protein efficiency ratio

PI:

Proximal intestine

PRV:

Protein retention value

PWG:

Percent weight gain

RGL:

Relative gut length

SGR:

Specific growth rate

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Acknowledgements

This research was financially supported by the National Basic Research Program of China (973 Program) (2014CB138600), National Natural Science Foundation of China (31572632), National Department Public Benefit Research Foundation (Agriculture) of China (201003020), Outstanding Talents and Innovative Team of Agricultural Scientific Research (Ministry of Agriculture), Science and Technology Support Program of Sichuan Province of China (2014NZ0003), Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China (2012NC0007; 2013NC0045), the Demonstration of Major Scientific and Technological Achievement Transformation Project of Sichuan Province of China (2015CC0011), Foundation of Sichuan Youth Science and Technology Innovation Research Team (2017TD0002) and Natural Science Foundation for Young Scientists of Sichuan Province (2014JQ0007). The authors would like to thank the personnel of these teams for their kind assistance.

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  1. Weiwei Xiao and Weidan Jiang contributed equally to this work.

Authors and Affiliations

  1. Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China

    Weiwei Xiao, Weidan Jiang, Lin Feng, Yang Liu, Pei Wu, Jun Jiang & Xiaoqiu Zhou

  2. Chengdu Mytech Biotech Co., Ltd., Chengdu, Sichuan, 610222, China

    Weiwei Xiao

  3. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China

    Weidan Jiang, Lin Feng, Yang Liu, Pei Wu, Jun Jiang & Xiaoqiu Zhou

  4. Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China

    Weidan Jiang, Lin Feng, Yang Liu, Pei Wu, Jun Jiang & Xiaoqiu Zhou

  5. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China

    Yongan Zhang

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  1. Weiwei Xiao
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Correspondence to Xiaoqiu Zhou.

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Xiao, W., Jiang, W., Feng, L. et al. Supplementation of enzyme-treated soy protein saves dietary protein and promotes digestive and absorptive ability referring to TOR signaling in juvenile fish. Fish Physiol Biochem 43, 1657–1675 (2017). https://doi.org/10.1007/s10695-017-0400-1

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