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Influences of glycyrrhetinic acid (GA) dietary supplementation on growth, feed utilization, and expression of lipid metabolism genes in channel catfish (Ictalurus punctatus) fed a high-fat diet

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Abstract

An 8-week feeding trial was performed to test the effects of glycyrrhetinic acid (GA) on growth and some gene expression of hepatic lipid metabolism in channel catfish (initial body weight, 3.5 ± 0.02 g) fed high-fat diets. Fish were fed the control diet, high-fat diet (HFD), and HFD supplemented with 0.4, 0.8, and 1.2 mg/kg GA in 15 tanks at a stocking density of 21 fish/tank. Fish fed HFD were significantly lower in body weight gain and specific growth rate but higher in feed intake and feed conversion ratio in comparison to the control. Supplement of GA at 1.2 mg/kg remarkably improved these parameters as compared to the control diet. High levels of cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and low levels of high-density lipoprotein (HDL) in plasma were observed in fish fed HFD; the opposite was observed for fish fed HFD supplemented with GA. The transcription of fatty acid synthase (FAS), sterol regulatory element-binding protein-1 (SREBP1), liver X receptor alpha (LXRα), and hormone-sensitive lipase (HSL) was upregulated, while that of carnitine palmitoyltransferase I (CPT1), peroxisome proliferator-activated receptors alpha (PPARα), acyl-CoA oxidase (ACO), and microsomal triglyceride transfer protein (MTTP) mRNA expression were downregulated in fish fed HFD. The opposite was observed in fish fed HFD supplemented with GA as well as the control group. In conclusion, supplementing the HFD with GA at 1.2 mg/kg could improve the growth performance and lipid metabolism of channel catfish consuming HFD.

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References

  • Abasubong KP, Li XF, Zhang DD, Jia ET, Xiang-Yang Y, Xu C, Liu WB (2018) Dietary supplementation of xylooligosaccharides benefits the growth performance and lipid metabolism of common carp (Cyprinus carpio) fed high-fat diets. Aquac Nutr 00:1–9. https://doi.org/10.1111/anu.12678

    Article  CAS  Google Scholar 

  • AOAC (1990) Official methods of analysis. Association of Official Analytical Chemists, Arlington, p 1141

    Google Scholar 

  • Armanini D, Nacamulli D, Francinipesenti F, Battagin G, Ragazzi E, Fiore C (2005) Glycyrrhetinic acid, the active principle of licorice, can reduce the thickness of subcutaneous thigh fat through topical application. Steroids 70:538–542

    Article  CAS  Google Scholar 

  • Beamish FWH, Medland TE (1986) Protein sparing effects in large rainbow trout, Salmo gairdneri. Aquaculture 55:35–42

    Article  Google Scholar 

  • Bromley PJ (1980) Effect of dietary protein, lipid, and energy content on the growth of turbot Scophthalmus maximus. Aquaculture 19:359–369

    Article  CAS  Google Scholar 

  • Chen QL, Luo Z, Huang C, Zheng JL, Pan YX, Song YF, Hu W (2015) Molecular cloning and tissue mRNA levels of 15 genes involved in lipid metabolism in Synechogobius hasta. Eur J Lipid Sci Technol 117:471–482

    Article  CAS  Google Scholar 

  • Chen QQ, Liu WB, Zhou M, Dai YJ, Xu C, Tian HY, Xu WN (2016) Effects of berberine on the growth and immune performance in response to ammonia stress and high-fat dietary in blunt snout bream Megalobrama amblycephala. Fish Shellfish Immunol 55:165–172

    Article  CAS  Google Scholar 

  • He ZS, Hu YL, Yin YL, Huang RL, Kong XF, Li TJ, Li FW, Wu GY (2007, January). Dietary supplementation with glycyrrhetinic acid (GA) increases endogenous arginine provision and growth performance in milk-fed piglets. In Journal of Dairy Science (Vol. 90, pp. 645-645). 1111 N DUNLAP AVE, SAVOY, IL 61874 USA: AMER DAIRY SCIENCE ASSOC

  • Hillestad M, Johnsen F (1994) High-energy/low-protein diets for Atlantic salmon: effects on growth, nutrient retention, and slaughter quality. Aquaculture 124:109–116

    Article  Google Scholar 

  • Horton JD, Goldstein JL, Brown MS (2002) SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J Clin Invest 109:1125–1131

    Article  CAS  Google Scholar 

  • Jiang GZ, Liu WB, Li GF, Wang M, Li XF (2012) Effects of different dietary glycyrrhetinic acid (GA) levels on growth, body composition, and plasma biochemical index of juvenile channel catfish, Ictalurus punctatus. Aquaculture 338–341:167–171

    Article  CAS  Google Scholar 

  • Jiang GZ, Zhou M, Zhang DD, Li XF, Liu WB (2018). The mechanism of action of a fat regulator: Glycyrrhetinic acid (GA) stimulating fatty acid transmembrane and intracellular transport in blunt snout bream (Megalobrama amblycephala). Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 226:83–90

  • Kalaiarasi P, Kaviarasan K, Pugalendi KV (2009) Hypolipidemic activity of 18beta-glycyrrhetinic acid on streptozotocin-induced diabetic rats. Eur J Pharmacol 612:93

    Article  CAS  Google Scholar 

  • Kim JB, Spiegelman BM (1996) ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism. Genes Dev 10:1096–1107

    Article  CAS  Google Scholar 

  • Kim YS, Park JW, Choi YJ (2010) New approaches for the effective recovery of fish proteins and their physicochemical characteristics. Fish Sci 69:1231–1239

    Article  Google Scholar 

  • Knopp RH, Superko HR, Davidson M, Insull W, Dujovne CA, Kwiterovich PO, Zavoral JH, Graham K, O'Connor RR, Edelman DA (1999) Long-term blood cholesterol-lowering effects of a dietary fiber supplement. Am J Prev Med 17:18–23

    Article  CAS  Google Scholar 

  • Kobayashi YMM, Kamei A, Abe K, Kojima T (2010) Ameliorative effects of mulberry (Morus alba L.) leaves on hyperlipidemia in rats fed a high-fat diet: induction of fatty acid oxidation, inhibition of lipogenesis, and suppression of oxidative stress. Biosci Biotechnol Biochem 74:2385–2395

    Article  CAS  Google Scholar 

  • Lefebvre P, Chinetti G, Fruchart JC, Staels B (2006) Sorting out the roles of PPARα in energy metabolism and vascular homeostasis. J Clin Investig 116:571–580

    Article  CAS  Google Scholar 

  • Lim E, Lim JY, Shin JH, Seok PR, Jung S, Yoo SH, Kim Y (2015) D-Xylose suppresses adipogenesis and regulates lipid metabolism genes in high-fat diet-induced obese mice. Nutr Res 35:626–636

    Article  CAS  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆CT method. Methods 25:402–408

    Article  CAS  Google Scholar 

  • Luo Q, Ouyang L (2010). Effect of glycyrrhizin on weight loss of experimental obese rats and its mechanism. Zhongnan Pharmaceutical Journal 3:50–54

  • Lusm LP, Eduardo D, MaríAteresa V, Mark D, Dominiquep B (2009) Effect of dietary lipid levels on performance, body composition, and fatty acid profile of juvenile white seabass, Atractoscion nobilis. Aquaculture 289:101–105

    Article  Google Scholar 

  • López LM, Torres AL, Durazo E, Drawbridge M, Bureau DP (2006). Effects of lipid on growth and feed utilization of white seabass (Atractoscion nobilis) fingerlings. Aquaculture, 253(1–4):557–563

    Article  Google Scholar 

  • Ma J, Shao Q, Xu Z, Zhou F (2013) Effect of dietary n-3 highly unsaturated fatty acids on growth, body composition, and fatty acid profiles of juvenile black seabream, Acanthopagrus schlegeli (Bleeker). J World Aquacult Soc 44:311–325

    Article  CAS  Google Scholar 

  • Mcgoogan BB, Dmiii G (1999) Dietary manipulations affecting growth and nitrogenous waste production of red drum, Sciaenops ocellatus I Effects of dietary protein and energy levels. Aquaculture 178:333–348

    Article  CAS  Google Scholar 

  • Mensinger AF, Walsh PJ, Hanlon RT (2005) Blood biochemistry of the oyster toadfish. J Aquat Anim Health 17:170–176

    Article  Google Scholar 

  • Minghetti M, Leaver MJ, Tocher DR (2011) Transcriptional control mechanisms of genes of lipid and fatty acid metabolism in the Atlantic salmon ( Salmo salar L.) established cell line, SHK-1. Biochim Biophys Acta 1811:194–202

    Article  CAS  Google Scholar 

  • Rigamonti E, Chinettigbaguidi G, Staels B (2008) Regulation of macrophage functions by PPAR-α, PPAR-γ, and LXRs in mice and men. Arterioscler Thromb Vasc Biol 28:1050–1059

    Article  CAS  Google Scholar 

  • Sargent JR, Tacon AG (1999) Development of farmed fish: a nutritionally necessary alternative to meat. Proc Nutr Soc 58:377–383

    Article  CAS  Google Scholar 

  • Stewart PM, Krozowski ZS (1997). 11β-Hydroxysteroid dehydrogenase. In Vitamins & Hormones (Vol. 57, pp. 249-324). Academic Press. https://doi.org/10.1016/S0083-6729(08)60646-9 

    Google Scholar 

  • Takeuchi-Yorimoto A, Noto T, Yamada A, Miyamae Y, Oishi Y, Matsumoto M (2013) Persistent fibrosis in the liver of choline-deficient and iron-supplemented L-amino acid-defined diet-induced nonalcoholic steatohepatitis rat due to continuing oxidative stress after choline supplementation. Toxicol Appl Pharmacol 268:264–277

    Article  CAS  Google Scholar 

  • Vergara JM, López-Calero G, Robaina L, Caballero MJ, Montero D, Izquierdo MS, Aksnes A (1999) Growth, feed utilization and body lipid content of gilthead seabream (Sparus aurata) fed increasing lipid levels and fish meals of different quality. Aquaculture 179:35–44

    Article  CAS  Google Scholar 

  • Watanabe T (1982) Lipid nutrition in fish. Comparative Biochemistry & Physiology Part A Molecular & Integrative Physiology 73B:3–15

    CAS  Google Scholar 

  • Wetterau JR, Aggerbeck LP, Bouma ME, Eisenberg C, Munck A, Hermier M, Schmitz J, Gay G, Rader DJ, Gregg RE (1992) Absence of microsomal triglyceride transfer protein in individuals with Abetalipoproteinemia. Science 258:999–1001

    Article  CAS  Google Scholar 

  • Xu L, Huang D, Hu Q, Wu J, Wang Y, Feng J (2015) Betaine alleviates hepatic lipid accumulation via enhancing hepatic lipid export and fatty acid oxidation in rats fed with a high-fat diet. Br J Nutr 113:1835–1843

    Article  CAS  Google Scholar 

  • Yoshii K, Takakuwa F, Nguyen HP, Masumoto T, Fukada H (2010) Effect of dietary lipid level on growth performance and feed utilization of juvenile kelp grouper Epinephelus bruneus. Fish Sci 76:139

    Article  CAS  Google Scholar 

  • Zar JH (1999) Biostatistical analysis. Q Rev Biol 18:797–799

    Google Scholar 

Download references

Acknowledgements

This research was supported by the earmarked fund for Jiangsu Agricultural Industry Technology System, Grant/Award Number: (JATS [2019]354). We also acknowledge the valuable help provided by students of our laboratory and Mr. Hossam-eldin Seddik for revising the grammar and providing suggestions.

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Tables and figures of this article can be found on the Figshare data repository and sharing site at https://figshare.com/s/121107d5d11748ceaca3

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Authors and Affiliations

  1. Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China

    Hesham Eed Desouky, Guang-zhen Jiang, Ding-dong Zhang, Kenneth Prudence Abasubong, Xiangyang Yuan, Xiang-fei Li & Wen-bin Liu

  2. Department of Animal, Poultry and Fish Production, Faculty of Agriculture, Damanhour University, Damanhour, Beheria, 22713, Egypt

    Hesham Eed Desouky

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  1. Hesham Eed Desouky
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  2. Guang-zhen Jiang
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  3. Ding-dong Zhang
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  4. Kenneth Prudence Abasubong
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  5. Xiangyang Yuan
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  6. Xiang-fei Li
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  7. Wen-bin Liu
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Contributions

Hesham Eed Desouky was responsible for the whole experiment, including feed formulation, culturing and applying treatments, performing different analyses, and writing the manuscript. Guang-zhen Jiang and Ding-dong Zhang contributed to set up the final experiment and gave suggestions during the pre-trial period. Kenneth Prudence Abasubong helped in the language revision of the manuscript. Xiangyang Yuan collaborated in formulating diets and laboratory analysis. Xiang-fei Li revised the statistical analysis. Wen-bin Liu revised the manuscript and gave suggestions.

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Correspondence to Wen-bin Liu.

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Desouky, H.E., Jiang, Gz., Zhang, Dd. et al. Influences of glycyrrhetinic acid (GA) dietary supplementation on growth, feed utilization, and expression of lipid metabolism genes in channel catfish (Ictalurus punctatus) fed a high-fat diet. Fish Physiol Biochem 46, 653–663 (2020). https://doi.org/10.1007/s10695-019-00740-4

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  • DOI: https://doi.org/10.1007/s10695-019-00740-4

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