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Conjugated linoleic acid alters growth performance, tissue lipid deposition, and fatty acid composition of darkbarbel catfish (Pelteobagrus vachelli)

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Abstract

Fatty liver syndrome is a prevalent problem of farmed fish. Conjugated linoleic acid (CLA) has received increased attention recently as a fat-reducing fatty acid to control fat deposition in mammals. Therefore, the aim of the present study was to determine whether dietary CLA can reduce tissue lipid content of darkbarbel catfish (Pelteobagrus vachelli) and whether decreased lipid content is partially due to alterations in lipid metabolism enzyme activities and fatty acid profiles. A 76-day feeding trial was conducted to investigate the effect of dietary CLA on the growth, tissue lipid deposition, and fatty acid composition of darkbarbel catfish. Five diets containing 0 % (control), 0.5 % (CLA0.5), 1 % (CLA1), 2 % (CLA2), and 3 % (CLA3) CLA levels were evaluated. Results showed that fish fed with 2–3 % CLA diets showed a significantly lower specific growth rate and feed conversion efficiency than those fed with the control diet. Dietary CLA decreased the lipid contents in the liver and intraperitoneal fat with the CLA levels from 1 to 3 %. Fish fed with 2–3 % CLA diets showed significantly higher lipoprotein lipase and hepatic triacylglycerol lipase activities in liver than those of fish fed with the control, and fish fed with 1–3 % CLA diets had significantly higher pancreatic triacylglycerol lipase activities in liver than those of fish fed with the control. Dietary CLA was incorporated into liver, intraperitoneal fat, and muscle lipids, with higher percentages observed in liver compared with other tissues. Liver CLA deposition was at the expense of monounsaturated fatty acids (MUFA). In contrast, CLA deposition appeared to be primarily at the expense of MUFA and n-3 polyunsaturated fatty acids (PUFA) in the intraperitoneal fat, whereas in muscle it was at the expense of n-3 PUFA. Our results suggested that CLA at a 1 % dose can reduce liver lipid content without eliciting any negative effect on growth rate in darkbarbel catfish. This lipid-lowering effect could be in part due to an increment in the activity of lipid metabolism enzymes and an extensive interconversion of fatty acids. Although CLA deposition in muscle (0.66–3.19 % of total fatty acids) are higher than presented in natural sources of CLA, EPA (C20:5n-3) in fish muscle appears simultaneously expendable, when the fish fed with 2–3 % CLA.

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Abbreviations

HTL:

Hepatic triacylglycerol lipase

HUFA:

Total highly unsaturated fatty acids

IPF:

Intraperitoneal fat ratio

LPL:

Lipoprotein lipase

MUFA:

Total monounsaturated fatty acids

PTL:

Pancreatic triacylglycerol lipase

PUFA:

Polyunsaturated fatty acids

SFA:

Total saturated fatty acids

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (Grant No. 31302195), the Natural Science Foundation of Hubei Province of China (Grant No. 2011CDB225), International Science & Technology Cooperation Program of China (Grant No. S2012ZR0135), the special fund for agro-scientific research in the public interest from the Ministry of Agriculture, China (201503242), and Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety.

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Authors and Affiliations

  1. Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan, 430023, Hubei, China

    Gui-Fang Dong, Wen-Zuo Liu, Lin-Zhou Wu, Deng-hang Yu & Feng Huang

  2. College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230000, Anhui, China

    Peng-Cheng Li & Yan-ou Yang

  3. Freshwater Aquaculture Collaborative Innovation Center in Hubei Province, Wuhan, 430070, Hubei, China

    Gui-Fang Dong & Feng Huang

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  1. Gui-Fang Dong
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  2. Wen-Zuo Liu
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  3. Lin-Zhou Wu
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  4. Deng-hang Yu
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  6. Peng-Cheng Li
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  7. Yan-ou Yang
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Correspondence to Yan-ou Yang.

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Dong, GF., Liu, WZ., Wu, LZ. et al. Conjugated linoleic acid alters growth performance, tissue lipid deposition, and fatty acid composition of darkbarbel catfish (Pelteobagrus vachelli). Fish Physiol Biochem 41, 73–89 (2015). https://doi.org/10.1007/s10695-014-0007-8

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