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Molecular characterization and expression patterns of two hormone-sensitive lipase genes in common carp Cyprinus carpio

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Abstract

The hormone-sensitive lipase (HSL) gene plays an important role in mammals’ lipid metabolism. Therefore, its function in fish is capturing increasing attention. In this study, two distinct cDNAs, designated HSL1 and HSL2, are firstly identified from common carp Cyprinus carpio. The full-length cDNA of HSL1 and HSL2 consists of 3379 bp and 2732 bp, encoding polypeptide of 693 and 847 amino acids, respectively, and shares 60.6% amino acid identity. Phylogenetic analysis suggests that HSL1 and HSL2 are derived from paralogous genes, which might have arisen during a teleost-specific genome duplication event. The two HSL mRNAs are differentially expressed, both in terms of distribution among tissues and in terms of abundance during embryogenesis. Moreover, both HSL mRNAs are expressed in various tissues, the highest in abdominal fat. Meanwhile, the two HSLs are detected at all stages of embryonic development, suggesting that they could be functional and involved in embryogenesis. In addition, the results show that the mRNA expression level of HSL2 in the high group of intramuscular fat content is significantly higher than that in the low group (P < 0.01). The research provides basic data for developing a further understanding of the function of HSL as well as molecular regulation mechanism in fat metabolism of common carp.

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Funding

This work was supported by the Central Public-interest Scientific Institution Basal Research Fund, HRFRI (HSY201805M), the National Natural Science Foundation of China (31302174), and the National Infrastructure of Fishery Germplasm Resources.

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Authors and Affiliations

  1. National Local Joint Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No.43 Songfa Street, Daoli District, Harbin, 150070, Heilongjiang Province, China

    Xianhu Zheng, Hongyang Dang, Weihua Lv, Zhipeng Sun, Youyi Kuang, Dingchen Cao, Cuiyun Lu & Xiaowen Sun

  2. Key Laboratory of Freshwater Fishery Germplasm Resource, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, No.999, Huchenghuan Road, Nanhui New City, Shanghai, 201306, China

    Hongyang Dang

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  1. Xianhu Zheng
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  2. Hongyang Dang
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Correspondence to Xianhu Zheng.

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All procedures were performed in accordance with the Guide for Care and Use of Laboratory Animals and approved by the Animal Care and Use Committee of Heilongjiang River Fisheries Research Institute.

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Zheng, X., Dang, H., Lv, W. et al. Molecular characterization and expression patterns of two hormone-sensitive lipase genes in common carp Cyprinus carpio. Fish Physiol Biochem 46, 439–450 (2020). https://doi.org/10.1007/s10695-019-00738-y

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