Skip to main content
SpringerLink
Log in

Administration of grape (Vitis vinifera) seed extract to rainbow trout (Oncorhynchus mykiss) modulates growth performance, some biochemical parameters, and antioxidant-relevant gene expression

  • Published:
Fish Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

Grape seed, as a main source of polyphenols, has many nutritional and medicinal properties in humans. In the current study, the effects of dietary ethanolic grape seed extract (GSE) on the growth performance, antioxidant activity, and some biochemical parameters in rainbow trout were investigated. Ninety fish (initial weight 78.47 g) were randomly distributed among nine cement tanks (1.8 m × 0.22 m × 0.35 m) with 10 fish per tank. Three experimental diets containing either 0, 10, or 50 g kg−1 GSE were prepared and each diet was randomly assigned to three tanks of fish for 60 days. Results showed that feeding GSE enhanced some growth parameters including the specific growth rate and condition factor in comparison with the control group. Among different serum metabolites, the glucose levels in treatment groups significantly decreased compared to the control group. The total product of lipid peroxidation indicated as malondialdehyde significantly decreased in both the GSE-added treatment groups. The gene expression related to the antioxidant enzymes, catalase, glutathione peroxidase 1, and glutathione S-transferase A, were upregulated in the intestine of fish that received a low dose of GSE. The results of the current study suggest that GSE, especially at 10 g kg−1, diet had the potential to improve (1) specific growth rate and condition factor, (2) biochemical parameters including glucose and lipid peroxidation product, and (3) upregulated the expression of antioxidant genes including catalase, glutathione peroxidase 1, and glutathione S-transferase A in rainbow trout.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Amar EC, Kiron V, Satoh S, Watanabe T (2004) Enhancement of innate immunity in rainbow trout (Oncorhynchus mykiss Walbaum) associated with dietary intake of carotenoids from natural products. Fish Shellfish Immunol 16:527–537

    Article  CAS  Google Scholar 

  • Arciuli M, Fiocco D, Fontana S, Arena MP, Frassanito MA, Gallone A (2017) Administration of a polyphenol-enriched feed to farmed sea bass (Dicentrarchus labrax L.): kidney melanomacrophages response. Fish Shellfish Immunol 68:404–410

    Article  CAS  Google Scholar 

  • Arslan G, Sönmez AY, Yanik T (2018) Effects of grape Vitis vinifera seed oil supplementation on growth, survival, fatty acid profiles, antioxidant contents and blood parameters in rainbow trout Oncorhynchus mykiss. Aquac Res. https://doi.org/10.1111/are.13686

  • Benzie IFF, Strain JJ (1996) Ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem 239:70–76

    Article  CAS  Google Scholar 

  • Bobe J, Marandel L, Panserat S, Boudinot P, Berthelot C, Quillet E, Volff JN, Genêt C, Jaillon O, Crollius HR, Guiguen Y (2016) The rainbow trout genome, an important landmark for aquaculture and genome evolution. In: MacKenzie S, Jentofi S (eds) Genomics in Aquaculture. Elsevier, Amsterdam, pp 21–43

    Chapter  Google Scholar 

  • Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30

    Article  CAS  Google Scholar 

  • Duong DN, Qin JG, Harris JO, Hoang TH, Bansemer MS, Currie KL, Phan-Thien KY, Dowell A, Stone DAJ (2016) Effects of dietary grape seed extract, green tea extract, peanut extract and vitamin C supplementation on metabolism and survival of greenlip abalone (Haliotis laevigata Donovan) cultured at high temperature. Aquaculture 464:364–373

    Article  CAS  Google Scholar 

  • Gonzalez-Paramas A, Esteban-Ruano S, Santos-Buelga C, Pascual-Teresa S, Rivas-Gonzalo J (2004) Flavanol content and antioxidant activity in winery byproducts. J Agric Food Chem 52:234–238

    Article  CAS  Google Scholar 

  • Harikrishnan R, Balasundaram C, Heo MS (2011) Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture 317:1–15

    Article  CAS  Google Scholar 

  • Jia R, Li Y, Cao L, Du J, Zheng T, Qian H, Gu Z, Jeney G, Xu P, Yin G (2019) Antioxidative, anti-inflammatory and hepatoprotective effects of resveratrol on oxidative stress-induced liver damage in tilapia (Oreochromis niloticus). Comp Biochem Physiol C 215:56–66

    CAS  Google Scholar 

  • Kao TT, Tu HC, Chang WN, Chen BH, Shi YY, Chang TC, Fu TF (2010) Grape seed extract inhibits the growth and pathogenicity of Staphylococcus aureus by interfering with dihydrofolate reductase activity and folate-mediated one-carbon metabolism. Int J Food Microbiol 141:17–27

    Article  CAS  Google Scholar 

  • Kruidenier L, Kuiper I, Lamers C, Verspaget HW (2003) Intestinal oxidative damage in inflammatory bowel disease: semi-quantification, localization, and association with mucosal antioxidants. J Pathol 201:28–36

    Article  CAS  Google Scholar 

  • Lange B, Currie KL, Howarth GS, Stone DAJ (2014) Grape seed extract and dried macroalgae, Ulva lactuca Linnaeus, improve survival of greenlip abalone, Haliotis laevigata Donovan, at high water temperature. Aquaculture 433:348–360

    Article  Google Scholar 

  • Laurent C, Besançon P, Caporiccio B (2007) Flavonoids from a grape seed extract interact with digestive secretions and intestinal cells as assessed in an in vitro digestion/Caco-2 cell culture model. Food Chem 100:1704–1712

    Article  CAS  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−∆∆Ct method. Methods 25:402–408

    Article  CAS  Google Scholar 

  • Mahdavikia F, Saharkhiz MJ (2015) Phytotoxic activity of essential oil and water extract of peppermint (Mentha×piperita L. CV. Mitcham). J Appl Res Med Aromat Plants 2(4):146–153

    Google Scholar 

  • Mandic AI, Đilas SM, Ćetković GS, Čanadanović-Brunet JM, Tumbas VT (2008) Polyphenolic composition and antioxidant activities of grape seed extract. Int J Food Prop 11:713–726

    Article  CAS  Google Scholar 

  • Martinez-Álvarez RM, Morales AE, Sanz A (2005) Antioxidant defenses in fish: biotic and abiotic factors. Rev Fish Biol Fish 15:75–88

    Article  Google Scholar 

  • Nakamura Y, Tsuji S, Tonogai Y (2003) Analysis of proanthocyanidins in grape seed extracts, health foods and grape seed oils. J Health Sci 49:45–54

    Article  CAS  Google Scholar 

  • Nootash S, Sheikhzadeh N, Baradaran B, Khani Oushani A, Maleki Moghadam MR, Nofouzi K, Monfaredan A, Aghebati L, Zare F, Shabanzadeh S (2013) Green tea (Camellia sinensis) administration induces expression of immune relevant genes and biochemical parameters in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol 35:1916–1923

    Article  CAS  Google Scholar 

  • Nowshehri JA, Bhat ZA, Shah MY (2015) Blessings in disguise: bio-functional benefits of grape seed extracts. Food Res Int 77:333–348

    Article  CAS  Google Scholar 

  • Revilla E, Escalona JM, Alonso E, Kovač V (1995) The phenolic composition of table grapes. In: Charalambous G (ed) Food flavors: Generation, analysis and process influence. Amsterdam: Elsevier Science, pp 1579–1596

  • Satho K (1978) Serum lipid peroxidation in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta 90:37–43

    Article  Google Scholar 

  • Sheikhzadeh N, Tayefi-Nasrabadi H, Khani Oushan A, Najafi Enferadi MH (2012) Effects of Haematococcus pluvialis supplementation on antioxidant system and metabolism in rainbow trout (Oncorhynchus mykiss). Fish Physiol Biochem 38:413–419

    Article  CAS  Google Scholar 

  • Shiel BP, Hall NE, Cooke IR, Robinson NA, Stone DAJ, Strugnell JM (2017) The effect of commercial, natural and grape seed extract supplemented diets on gene expression signatures and survival of greenlip abalone (Haliotis laevigata) during heat stress. Aquaculture 479:798–807

    Article  CAS  Google Scholar 

  • Singleton VL, Orthofer R, Lamuela-Raventos RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods Enzymol 299:152–178

    Article  CAS  Google Scholar 

  • Uchida S (1980) Condensed tannins scavenging active oxygen radicals. Med Sci Res 15:831–832

    Google Scholar 

  • Verlhac Trichet V (2010) Nutrition and immunity: an update. Aquac Res 41:356–372

    Article  Google Scholar 

  • Viveros A, Chamorro S, Pizarro M, Arija I, Centeno C, Brenes A (2011) Effects of dietary polyphenol-rich grape products on intestinal microflora and gut morphology in broiler chicks. Poult Sci 90:566–578

    Article  CAS  Google Scholar 

  • Xie LN, Mao TT, Liu C, Jiang LS, Guo YQ, Fang LY (2012) Effect of GSP on enzyme activity, relative weight of intestinal organs and routine blood test for weaned piglets. J Beijing Uni Agric 27:17–19

    Google Scholar 

  • Yamakoshi J, Tokutake S, Kikuchi M, Kubota Y, Konishi H, Mitsuoka T (2001) Effect of proanthocyanidin-rich extract from grape seeds on human fecal flora and fecal odor. Microb Ecol Health Dis 13:25–31

    CAS  Google Scholar 

  • Zhai SW, Lu JJ, Chen XH (2014) Effects of dietary grape seed proanthocyanidins on growth performance, some serum biochemical parameters and body composition of tilapia (Oreochromis niloticus) fingerlings. Ital J Anim Sci 13:536–540

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are thankful to the Research affairs of the University of Tabriz for the financial support of this project.

Author information

Authors and Affiliations

  1. Department of Food Hygiene and Aquatic Animals, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

    Shalaeh Mousavi & Najmeh Sheikhzadeh

  2. Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

    Hossein Tayefi-Nasrabadi

  3. Department of Horticultural Science, University of Tabriz, Tabriz, Iran

    Saeedeh Alizadeh-Salteh

  4. Department of Fisheries, Sciences and Research Branch, Islamic Azad University, Tehran, Iran

    Ali Khani Oushani

  5. Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

    Masoumeh Firouzamandi

  6. Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

    Karim Mardani

Authors
  1. Shalaeh Mousavi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Najmeh Sheikhzadeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hossein Tayefi-Nasrabadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Saeedeh Alizadeh-Salteh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ali Khani Oushani
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Masoumeh Firouzamandi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Karim Mardani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shalaeh Mousavi.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mousavi, S., Sheikhzadeh, N., Tayefi-Nasrabadi, H. et al. Administration of grape (Vitis vinifera) seed extract to rainbow trout (Oncorhynchus mykiss) modulates growth performance, some biochemical parameters, and antioxidant-relevant gene expression. Fish Physiol Biochem 46, 777–786 (2020). https://doi.org/10.1007/s10695-019-00716-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10695-019-00716-4

Keywords

Search

Navigation