Abstract
The 14-3-3 proteins are a family of widely expressed acidic proteins, which are involved in the regulation of many biological processes of animals. However, no research regarding 14-3-3 has been described in sturgeon to date, one of the most primitive Actinopterygii species. Here, we identified the first 14-3-3 gene from Siberian sturgeon (Acipenser baeri), named Ab14-3-3β/α (GenBank Accession No. KY094076.1). The cDNA of Ab14-3-3β/α is 1212 bp in length, containing a 5′-untranslated region (UTR) of 82 bp, a 3′UTR of 392 bp, and an open reading frame (ORF) of 738 bp, encoding a polypeptide of 245 amino acids which contains a 14-3-3 homologs domain (PF00244). Phylogenetic analysis showed that the 14-3-3 gene product from Acipenser baeri is a counterpart of vertebrate 14-3-3β/α. The deduced Ab14-3-3β/α protein shares high identities of 46.5–95.5% with the homologs of other species. Ab14-3-3β/α mRNA was constitutively expressed in all examined tissues, with high expression levels in the blood and gill. Furthermore, the expression level of Ab14-3-3β/α mRNA increased significantly in the gill at 1 h under acute salinity shock by transfer of Siberian sturgeons from fresh water (FW) to 15 ppt. In fish subjected to a high temperature (31 °C), Ab14-3-3β/α showed a significant upregulation in the liver at 3 h compared with the control group (24 °C). A 4.85-fold increase of Ab14-3-3β/α expression in the spleen of Siberian sturgeon was observed at 24 h following Aeromonas hydrophila challenge. Collectively, these results indicated that Ab14-3-3β/α might play a certain role in sturgeon in response to some environmental stresses and bacterial challenge.
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Funding
This study was funded by Beijing Municipal Excellent Talents Foundation (2017000020060G122), Foundation of Beijing Municipal Science and Technology Project (Z161100004516003), Natural Science Foundation of China (31602137), and Beijing Sturgeon and Trout Innovation Team (BAIC08-2019).
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The institutional Animal Care and Use Committee at the Beijing Fisheries Research Institute approved this study. The experimental procedures were performed with strict accordance with the standards of the Chinese Council on Animal Care.
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Wang, X., Ma, G. & Zhu, H. Regulation of 14-3-3β/α gene expression in response to salinity, thermal, and bacterial stresses in Siberian sturgeon (Acipenser baeri). Fish Physiol Biochem 46, 519–531 (2020). https://doi.org/10.1007/s10695-019-00702-w
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DOI: https://doi.org/10.1007/s10695-019-00702-w