Abstract
An ovarian cell line was successfully developed from the juvenile ovary of Southern catfish (SCO1) (Silurus meridionalis), which was designated as SCO1. The cell line multiplied preferentially in L-15 medium with 15 % fetal bovine serum at 28 °C for more than 70 passages over a period of 420 days. SCO1 showed fibroblast-like morphology and predominantly retained a diploid karyotype of 58 chromosomes. From the gene expression patterns, SCO1 showed a characteristic of ovarian granulosa cells. After the cells were transfected with the green fluorescent protein expression vector, bright fluorescent signals could be observed in approximately 30 % cells. This cell line may be valuable for the evaluation of endocrine disruptors and studying interactions between somatic cells and germ cells.
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Acknowledgments
This work was supported by Grant 2012CB723205 from the National Basic Research Program of China; Grant 31201979 from the National Natural Science Foundation of China; Grant cstc2012jjA80041 from the Natural Science Foundation Project of CQ CSTC.
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Wei, J., Qi, W., Zhou, Y. et al. Establishment and characterization of an ovarian cell line from Southern catfish (Silurus meridionalis). Fish Physiol Biochem 40, 1383–1391 (2014). https://doi.org/10.1007/s10695-014-9932-9
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DOI: https://doi.org/10.1007/s10695-014-9932-9