Abstract
Fruit shape is one of the important quantitative traits in pear (Pyrus spp.) breeding program, thus genetic study related to fruit shape could be beneficial to pear breeding. Quantitative trait loci (QTL) analysis was carried out using ‘Whangkeumbae’ (P. pyrifolia, round) × ‘Yali’ (P. bretschneideri, pyriform) and high-resolution melting (HRM) markers were developed. The genetic linkage map of ‘Whangkeumbae’ × ‘Yali’ was constructed using single nucleotide polymorphisms (SNPs) derived from Axiom Pear 70 K Genotyping Array and simple sequence repeats. The integrated genetic linkage map of ‘Whangkeumbae’ × ‘Yali’ showed ~ 90% of genome coverage, a total genetic distance of 998.2 cM, and a marker density of 1.6 cM. F1 progenies of ‘Whangkeumbae’ × ‘Yali’ showed normal distribution of fruit length (L), diameter (D), and L/D ratio. Three QTLs located in linkage group (LG) 6, 7, and 12 were identified with LOD thresholds of 2.8–3.0. Six HRM markers were developed using array-SNPs anchored in the QTLs and predicted fruit shape with 28.6–65.3% accuracy. Notably, accuracy was increased by ~ 90% using an HRM marker combination consisting of CBp06sn02, CBp07sn01, and CBp12sn03. These results could provide a better understanding of the genetic mechanism of fruit shape development and reducing pear breeding period.
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Acknowledgements
This work was carried out with the support of “Cooperative Research Program for Agriculture Science and Technology Development (No. PJ015682)” Rural Development Administration, Republic of Korea.
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SC and SO performed the overall experiments, data analysis, and wrote the manuscript together. HH contributed to bioinformatics analysis. KK and HJ contributed to phenotyping. DK designed and managed whole experiments and finalized the manuscript.
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Sun-Heum Cho and Sewon Oh authors contributed equally to this work.
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Cho, SH., Oh, S., Han, H. et al. QTL analysis and development of HRM markers associated with fruit shape in interspecific pears (Pyrus pyrifolia × P. bretschneideri). Euphytica 218, 122 (2022). https://doi.org/10.1007/s10681-022-03079-9
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DOI: https://doi.org/10.1007/s10681-022-03079-9