Abstract
Breeding at both interspecific and intergeneric levels is an effective method for expanding genetic variation of cruciferous crops; however, few commercially accepted varieties have been released because of difficulties in generating fertile progenies. We employed backcross strategy to introduce Eruca vesicaria-specific characteristics into Brassica rapa and successfully obtained four BC1F1 lines that showed a wide range of diversity of morphological traits and glucosinolate (GSL) composition. Thus, leaf shape, anthocyanin coloration, glucoraphanin content, and red petal veins were found to be inherited mainly from E. vesicaria, though most of the morphological characteristics and GSL composition were inherited from intergeneric F1 plants. Since all BC1F1 lines showed post-fertilization barriers, backcrossing with B. rapa was performed and BC2F1 progenies were successfully obtained, which showed diverse morphological characteristics and GSL composition as well as higher regeneration potential. These results suggest that widening of genetic diversity of B. rapa can be achieved through successive reciprocal backcrossing of B. rapa × E. vesicaria hybrids with B. rapa.
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Acknowledgements
The authors would like to thank Pro. Bang at Utsunomiya University, Mr. Serizawa at the Nagano Vegetable and Ornamental Crops Experiment Station, Mr. Yoshida and Mr. Tomita at Kanagawa Agricultural Technology Center, Ms. Miyazawa and Ms. Kai at Kanagawa Prefectural Institute of Public Health. We would like to extend our special thanks to the technical staffs, Ms. Takahashi and Ms. Nakata at Kanagawa Agricultural Technology Center. The authors would like to thank Enago (www.enago.jp) for the English language review.
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AK: developed the plants, conducted the experiments, and analyzed the data. FM: partly participated in the experiments. NK: conceived the project, designed the research, and critically reviewed and improved the final manuscript.
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Kaminishi, A., Miyohashi, F. & Kita, N. Diversity of morphological traits and glucosinolate composition in backcross progenies of Brassica rapa L. × Eruca vesicaria (L.) Cav. hybrids. Euphytica 218, 114 (2022). https://doi.org/10.1007/s10681-022-03056-2
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DOI: https://doi.org/10.1007/s10681-022-03056-2