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Hybridisation within Brassica and allied genera: evaluation of potential for transgene escape

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Abstract

Determining the potential for hybridisation between transgenic crops and their relatives is a major component of risk assessment. Recent assessments of the extent of reproductive compatibility between crops and their relatives draw heavily on existing data from experimental crosses to infer the likelihood of hybridisation and introgression. Since the literature in this area continues to grow at a rapid pace, it is essential that such analyses can be easily updated. We used a database approach to assemble data on reproductive compatibility for eight crop species in Brassica, Raphanus and Sinapis, using data from hand pollination, spontaneous (unassisted) pollination and trials using in vitro techniques (e.g. embryo rescue), incorporating 326 studies and 216 species combinations. We found many reports for major crop species (B. juncea, B. napus, B. oleracea and B. rapa), but fewer for minor crops (B. carinata, B. nigra, Raphanus sativus and Sinapis alba). Many species combinations remain untested, and we highlight these information gaps. While reproductively incompatible species can be discounted as targets for transgene escape, compatible species must be evaluated further in the particular context where transgenic crops are grown. Because the data is retained in a database in a relatively unmodified form, multiple views of the data can be generated; this review represents one possible view of this data. Our approach also allows new data to be easily incorporated into future reanalyses and can be extended to other crop groups, and as such is a useful method of assembling, analysing and sharing data for risk assessment.

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References

  • AGBIOS (2005) GM Database: Information on GM approved products. Available: http://www.agbios.com/dbase.php

  • Agnihotri A, Gupta VK, Lakshmikumaran MS et al (1990) Production of Eruca-Brassica hybrids by embryo rescue. Plant Breed 104:281–289

    Google Scholar 

  • Allan Herbarium (2000) New Zealand plant names database. Landcare Research, New Zealand. Available: http://nzflora.landcareresearch.co.nz

  • Anand IJ, Mishra PK, Rawat DS (1985) Mechanism of male sterility in Brassica juncea. I. Manifestation of sterility and fertility restoration. Cruciferae Newsl 10:44–46

    Google Scholar 

  • Apel P, Bauwe H, Ohle H (1984) Hybrids between Brassica alboglabra and Moricandia arvensis and their photosynthetic properties. Biochem Physiol Pflanz 179:793–797

    CAS  Google Scholar 

  • Armstrong TT, FitzJohn RG, Newstrom LE, Wilton AD, Lee WG (2005) Transgene escape: what potential for crop–wild hybridization? Mol Ecol 14:2111–2132

    PubMed  CAS  Google Scholar 

  • Arnold ML (1997) Natural hybridisation and evolution. Oxford University Press, New York

    Google Scholar 

  • Ayotte R, Harney PM, Souza Machado V (1987) The transfer of triazine resistance from Brassica napus L. to B. oleracea L. I. Production of F1 hybrids through embryo rescue. Euphytica 36:615–624

    Google Scholar 

  • Bang SW, Kaneko Y, Matsuzawa Y (1996) Production of intergeneric hybrids between Raphanus and Sinapis and the cytogenetics of their progenies. Breed Sci 46:45–51

    Google Scholar 

  • Bang SW, Iida D, Kaneko Y, Matsuzawa Y (1997) Production of new intergeneric hybrids between Raphanus sativus and Brassica wild species. Breed Sci 47:223–228

    CAS  Google Scholar 

  • Banga SS, Labana KS (1991) Cytoplasmic-genetic relationship between Brassica nigra and Sinapis allioni. Cruciferae Newsl 14/15:12–13

    Google Scholar 

  • Baranger A, Chèvre AM, Eber F, Renard M (1995) Effect of oilseed rape genotype on the spontaneous hybridization rate with a weedy species: an assessment of transgene dispersal. Theor Appl Genet 91:956–963

    CAS  Google Scholar 

  • Barcikowska B, Balicka M, Zwierzykowska E (1983) On the way to yellow seeded Brassica napus. I. Crossings between Brassica oleracea and B. carinata. Cruciferae Newsl 8:20

    Google Scholar 

  • Bijral JS, Sharma TR (1995) Intergeneric (intertribe) hybridization between Rorippa islandica, a wild species, and Brassica napus. Plant Genet Resour Newsl 101:20

  • Bijral JS, Sharma TR (1996a) Cytogenetics of intergeneric hybrids between Brassica napus L. and Eruca sativa Lam Cruciferae Newsl 18:12–13

    Google Scholar 

  • Bijral JS, Sharma TR (1996b) Intergeneric hybridization between Brassica napus and Diplotaxis muralis. Cruciferae Newsl 18:10–11

    Google Scholar 

  • Bijral JS, Sharma TR (1998) Production and cytology of intergeneric hybrids between Brassica napus and Diplotaxis catholica. Cruciferae Newsl 20:15

    Google Scholar 

  • Bijral JS, Sharma TR (1999a) Brassica juncea-Eruca sativa sexual hybrids. Cruciferae Newsl 21:33–34

    Google Scholar 

  • Bijral JS, Sharma TR (1999b) Morpho-cytogenetics of Brassica juncea × Brassica oxyrrhina hybrids. Cruciferae Newsl 21:35–36

    Google Scholar 

  • Bijral JS, Gupta BB, Singh K, Sharma TR (1991) Interspecific hybridization between Brassica juncea (L.) Czern & Coss and Brassica hirta Moench. Indian J Genet Plant Breed 51:476–478

    Google Scholar 

  • Bijral JS, Sharma TR, Gupta BB, Singh K (1995) Interspecific hybrids of Brassica maurorum with Brassica crops and their cytology. Cruciferae Newsl 17:18–19

    Google Scholar 

  • Bing DJ, Downey RK, Rakow GFW (1996a) Assessment of transgene escape from Brassica rapa (B. campestris) into B. nigra or Sinapis arvensis. Plant Breed 115:1–4

    Google Scholar 

  • Bing DJ, Downey RK, Rakow GFW (1996b) Hybridizations among Brassica napus, B. rapa and B. juncea and their two weedy relatives B. nigra and Sinapis arvensis under open pollination conditions in the field. Plant Breed 115:470–473

    Google Scholar 

  • Bourdôt G, Conner AJ, Jenkins T (1999) Ecological risks and managerial consequences of Roundup ReadyTM oilseed rape in New Zealand. Institute for Crop and Food Research, Christchurch, New Zealand

    Google Scholar 

  • Brown AP, Brown J, Thill DC, Brammer TA (1996) Gene transfer between canola (Brassica napus) and related weed species. Cruciferae Newsl 18:36–37

    Google Scholar 

  • Calder RA (1937) Interpollination of Brassicas: its significance in relation to seed production. N Z J Agric 55:299–308

    Google Scholar 

  • Chen BY, Heneen WK (1992) Inheritance of seed colour in Brassica campestris L. and breeding for yellow-seeded B. napus L Euphytica 59:157–163

    Google Scholar 

  • Cheng BF, Séguin-Swartz G (2000) Meiotic studies on intergeneric hybrids between Brassica napus and Orychophragmus violaceus. Cruciferae Newsl 22:11–12

    Google Scholar 

  • Chèvre AM, Eber F, Baranger A et al (1996) Interspecific gene flow as a component of risk assessment for transgenic Brassicas. Acta Hort 407:169–179

    Google Scholar 

  • Chèvre AM, Eber F, Baranger A et al (1998a) Characterization of backcross generations obtained under field conditions from oilseed rape-wild radish F1 interspecific hybrids: an assessment of transgene dispersal. Theor Appl Genet 97:90–98

    Google Scholar 

  • Chèvre AM, Eber F, Baranger A et al (1998b) Risk assessment on crucifer species. Acta Hort 459:219–224

    Google Scholar 

  • Chèvre AM, Eber F, Darmency H et al (2000) Assessment of interspecific hybridization between transgenic oilseed rape and wild radish under normal agronomic conditions. Theor Appl Genet 100:1233–1239

    Google Scholar 

  • Chèvre AM, Ammitzbøll H, Breckling B et al (2004) A review on interspecific gene flow from oilseed rape to wild relatives. In: den Nijs HCM, Bartsch D, Sweet J (eds) Introgression from genetically modified plants into wild relatives. CAI International, Cambridge, pp 235–251

    Google Scholar 

  • Chiang MS, Chiang BY, Grant WF (1977) Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. oleracea var. capitata) I. Interspecific hybridization between B. napus and B. oleracea var. capitata. Euphytica 26:319–336

    Google Scholar 

  • Choudhary BR, Joshi P (1999) Interspecific hybridization in Brassica. In: Proceedings of the 10th international rapeseed congress, Canberra, Australia. Contrib No. 516

  • Choudhary BR, Joshi P (2000) Cytomorphology of intergeneric hybrid Sinapis alba × Brassica nigra. J Genet Breed 54:157–160

    Google Scholar 

  • Choudhary BR, Joshi P (2001) Crossability of Brassica tournefortii and B. rapa, and morphology and cytology of their F1 hybrids. Theor Appl Genet 102:1123–1128

    Google Scholar 

  • Choudhary BR, Joshi P, Ramarao S (2000a) Interspecific hybridization between Brassica carinata and Brassica rapa. Plant Breed 119:417–420

    Google Scholar 

  • Choudhary BR, Joshi P, Singh K (2000b) Synthesis, morphology and cytogenetics of Raphanofortii (TTRR, 2n=38): a new amphidiploid of hybrid Brassica tournefortii (TT, 2n=20) × Raphanus caudatus (RR, 2n=18). Theor Appl Genet 101:990–999

    Google Scholar 

  • Chrungu B, Verma N, Mohanty A, Pradhan A, Shivanna KR (1999) Production and characterization of interspecific hybrids between Brassica maurorum and crop brassicas. Theor Appl Genet 98:608–613

    Google Scholar 

  • Daniels R, Boffey C, Mogg R, Bond J, Clarke R (2005) The potential for dispersal of herbicide tolerance genes from genetically-modified, herbicide-tolerant oilseed rape crops to wild relatives. Report to the Department for the Environment, Food and Rural Affairs (DEFRA) No. EPG 1/5/151. Available: http://www.defra.gov.uk/environment/gm/research/pdf/epg_1–5–151.pdf

  • Darmency H, Fleury A (2000) Mating system in Hirschfeldia incana and hybridization to oilseed rape. Weed Res 40:231–238

    Google Scholar 

  • Darmency H, Lefol E, Fleury A (1998) Spontaneous hybridizations between oilseed rape and wild radish. Mol Ecol 7:1467–1473

    Google Scholar 

  • Davey VM (1959) Cultivated Brassiceae: information available to the breeder. In: Scottish plant breeding report, pp 23–62

  • Dayal N (1987) Towards synthesis of ×Raphanoeruca. Cruciferae Newsl 12:7

    Google Scholar 

  • Diederichsen E, Sacristan MD (1988) Interspecific hybridizations in the genus Brassica followed by in ovule embryo culture. Cruciferae Newsl 13:20–21

    Google Scholar 

  • Eber F, Chèvre AM, Baranger A et al (1994) Spontaneous hybridization between a male-sterile oilseed rape and two weeds. Theor Appl Genet 88:362–368

    Google Scholar 

  • Eenink AH (1974) Matromorphy in Brassica oleracea L. III. The influence of temperature, delayed prickle pollination and growth regulators on the number of matromorphic seeds formed. Euphytica 23:711–718

    Google Scholar 

  • Ellerström S (1978) Species crosses and sterility in Brassica and Raphanus. Cruciferae Newsl 3:16–17

    Google Scholar 

  • Ellerström S, Zagorcheva L (1977) Sterility and apomictic embryo-sac formation in Raphanobrassica. Hereditas 87:107–119

    Google Scholar 

  • Ellstrand NC (2003) Dangerous liaisons? When cultivated plants mate with their wild relatives. Johns Hopkins University Press, Baltimore, MD

    Google Scholar 

  • Fan Z, Tai W, Stefansson BR (1985) Male sterility in Brassica napus L. associated with an extra chromosome. Can J Genet Cytol 27:467–471

    Google Scholar 

  • Fernandez-Escobar J, Dominguez J, Martin A, Fernandez-Martinez JM (1988) Genetics of the erucic acid content in interspecific hybrids of Ethiopian mustard (Brassica carinata Braun) and rapeseed (B. napus L.). Plant Breed 100:310–315

    CAS  Google Scholar 

  • Frandsen KJ (1947) The experimental formation of Brassica napus L. var. oleifera D.C. and Brassica carinata Braun. Dansk Bot Ark 12:1–16

    Google Scholar 

  • Frello S, Hansen KR, Jensen J, Jørgensen RB (1995) Inheritance of rapeseed (Brassica napus)-specific RAPD markers and a transgene in the cross B. juncea × (B. juncea × B. napus). Theor Appl Genet 91:236–241

    Google Scholar 

  • Getinet A, Rakow G, Raney JP, Downey RK (1994) Development of zero erucic acid Ethiopian mustard through an interspecific cross with zero erucic acid Oriental mustard. Can J Plant Sci 74:793–795

    CAS  Google Scholar 

  • Getinet A, Rakow G, Raney JP, Downey RK (1997) Glucosinolate content in interspecific crosses of Brassica carinata with B. juncea and B. napus. Plant Breed 116:39–46

    CAS  Google Scholar 

  • Ghosh Dastidar N, Varma NS (1999) A study on intercrossing between transgenic B. juncea and other related species. In: Proceedings of the 10th international rapeseed congress, Canberra, Australia. Contrib No. 244

  • Goswami R, Devi J (2002) Intergeneric hybridization of Indian mustard (Brassica juncea) with taramira (Eruca sativa). Indian J Agric Sci 72:436–438

    Google Scholar 

  • Goyal RK, Chowdhury JB, Jain RK (1997) Development of fertile Brassica juncea × B. tournefortii hybrids through embryo rescue. Cruciferae Newsl 19:19–20

    Google Scholar 

  • Grant V (1994) Modes and origins of mechanical and ethological isolation in angiosperms. Proc Natl Acad Sci USA 91:3–10

    PubMed  CAS  Google Scholar 

  • Gundimeda HR, Prakash S, Shivanna KR (1992) Intergeneric hybrids between Enarthrocarpus lyratus, a wild species, and crop brassicas. Theor Appl Genet 83:655–662

    Google Scholar 

  • Halfhill MD, Millwood RJ, Raymer PL, Stewart CN Jr (2002) Bt-transgenic oilseed rape hybridization with its weedy relative, Brassica rapa. Environ Biosafety Res 1:19–28

    PubMed  Google Scholar 

  • Halfhill MD, Zhu B, Warwick SI et al (2004) Hybridization and backcrossing between transgenic oilseed rape and two related weed species under field conditions. Environ Biosafety Res 3:73–81

    PubMed  Google Scholar 

  • Hansen LB, Siegismund HR, Jørgensen RB (2001) Introgression between oilseed rape (Brassica napus L.) and its weedy relative B. rapa L. in a natural population. Genet Resour Crop Evol 48:621–627

    Google Scholar 

  • Hemingway JS (1995) Mustards – Brassica spp. and Sinapis alba (Cruciferae). In: Smartt J, Simmonds NW (eds) Evolution of crop plants. Longman Scientific and Technical, Harlow, pp 82–86

    Google Scholar 

  • Heyn FW (1977) Analysis of unreduced gametes in the Brassiceae by crosses between species and ploidy levels. Z Pflanzenzücht 78:13–30

    Google Scholar 

  • Honma S, Summers WL (1976) Interspecific hybridization between Brassica napus L. (Napobrassica group) and B. oleracea L. (Botrytis group). J Am Soc Hort Sci 101:299–302

    Google Scholar 

  • Howard HW (1942) The effect of polyploidy and hybridity on seed size in crosses between Brassica chinensis, B. carinata, amphidiploid B. chinensis-carinata and autotetraploid B. chinensis. J Genet 43:105–119

    Google Scholar 

  • Huang B, Liu Y, Wu W, Xue X (2002) Production and cytogenetics of intergeneric hybrids between Ogura CMS Brassica napus and Raphanus sativus. Cruciferae Newsl 24:25–27

    CAS  Google Scholar 

  • Inomata N (1988) Intergeneric hybridization between Brassica napus and Sinapis arvensis and their crossability. Cruciferae Newsl 13:22–23

    Google Scholar 

  • Inomata N (1994) Intergeneric hybridization between Brassica napus and Sinapis pubescens, and the cytology and crossability of their progenies. Theor Appl Genet 89:540–544

    Google Scholar 

  • Inomata N (1998) Production of the hybrids and progenies in the intergeneric cross between Brassica juncea and Diplotaxis erucoides. Cruciferae Newsl 20:17–18

    Google Scholar 

  • Inomata N (2000) Interspecific hybridization between Brassica campestris and B. spinescens and the cytogenetic analysis of their progenies. Cruciferae Newsl 22:13–14

    Google Scholar 

  • Inomata N (2001) Intergeneric hybridization between Brassica juncea and Erucasturum virgatum and the meiotic behavior of F1 hybrids. Cruciferae Newsl 23:17–18

    Google Scholar 

  • James C (2004) Preview: global status of commercialized Biotech/GM crops: 2004. ISAAA, Ithaca, NY. Available: http://www.isaaa.org/kc/CBTNews/press_release/briefs32/ESummary/Executive%20Summary%20(English).pdf

  • Jenkins TE, Conner AJ, Frampton CM (2001) Investigating gene introgression from rape to wild turnip. N Z Plant Protect 54:101–104

    Google Scholar 

  • Jørgensen RB, Andersen B (1994) Spontaneous hybridization between oilseed rape (Brassica napus) and weedy B. campestris (Brassicaceae) a risk of growing genetically modified oilseed rape. Am J Bot 81:1620–1626

    Google Scholar 

  • Jørgensen RB, Andersen B, Hauser TP et al (1998) Introgression of crop genes from oilseed rape (Brassica napus) to related wild species – an avenue for the escape of engineered genes. Acta Hort 459:211–217

    Google Scholar 

  • Joshi P, Choudhary BR (1999) Interspecific hybridization in Brassica. I. B. carinata × B. tournefortii. In: Proceedings of the 10th international rapeseed congress, Canberra, Australia. Contrib No. 517

  • Kamala T (1983) A study on the cytogenetic homeologies between Raphanus and Brassica genomes. Indian J Bot 6:131–140

    Google Scholar 

  • Karpechenko GD (1924) Hybrids of (female) Raphanus sativus L. × (male) Brassica oleracea L J Genet 14:375–396

    Google Scholar 

  • Katiyar RK, Chamola R (1995) Useful end products from Brassica juncea × B. carinata and Brassica juncea × B. campestris crosses. Cruciferae Newsl 17:20–21

    Google Scholar 

  • Katiyar RK, Gupta VK (1987) Root tumours in interspecific cross of Brassica species. Indian J Agric Sci 57:927–930

    Google Scholar 

  • Kerlan MC, Chèvre AM, Eber F, Baranger A, Renard M (1992) Risk assessment of outcrossing of transgenic rapeseed to related species: I. Interspecific hybrid production under optimal conditions with emphasis on pollination and fertilization. Euphytica 62:145–153

    Google Scholar 

  • Landbo L, Andersen B, Jørgensen RB (1996) Natural hybridisation between oilseed rape and a wild relative: hybrids among seeds from weedy B. campestris. Hereditas 125:89–91

    Google Scholar 

  • Leckie D, Smithson A, Crute IR (1993) Gene movement from oilseed rape to weedy populations – a component of risk assessment for transgenic cultivars. Aspects Appl Biol 35:61–66

    Google Scholar 

  • Lefol E, Danielou V, Darmency H et al (1991) Escape of engineered genes from rapeseed to wild Brassiceae. In: Proc. Brighton crop protect conf., Weeds, pp 1049–1056

  • Lefol E, Danielou V, Darmency H (1996a) Predicting hybridization between transgenic oilseed rape and wild mustard. Field Crops Res 45:153–161

    Google Scholar 

  • Lefol E, Fleury A, Darmency H (1996b) Gene dispersal from transgenic crops. II. Hybridization between oilseed rape and the wild hoary mustard. Sex Plant Reprod 9:189–196

    Article  Google Scholar 

  • Lefol E, Séguin-Swartz G, Downey RK (1997) Sexual hybridisation in crosses of cultivated brassica species with the crucifers Erucastrum gallicum and Raphanus raphanistrum – potential for gene introgression. Euphytica 95:127–139

    Google Scholar 

  • Li Z, Heneen WK (1999) Production and cytogenetics of intergeneric hybrids between the three cultivated Brassica diploids and Orychophragmus violaceus. Theor Appl Genet 99:694–704

    Google Scholar 

  • Li Z, Wu JG, Liu Y, Liu HL, Heneen WK (1998) Production and cytogenetics of the intergeneric hybrids Brassica juncea × Orychophragmus violaceus and B.carinata × O. violaceus. Theor Appl Genet 96:251–265

    Google Scholar 

  • Ljungberg A, Cheng B, Heneen WK (1993) Investigation of hybrids between Brassica tournefortii Gouan and B. alboglabra Bailey. Sver Utsädesfören Tidsk 103:191–197

    Google Scholar 

  • Luo P, Yin JM, Wu YY, Lan ZQ (2000) Studies on chromosome behaviour in intergeneric hybrids of some cruciferous plants. Cruciferae Newsl 22:17–18

    Google Scholar 

  • Mackay GR (1973) Interspecific hybrids between forage rape (Brassica napus L.) and turnip (Brassica campestris L. spp. rapifera) as alternatives to forage rape. 1. An exploratory study with single pair crosses. Euphytica 22:495–499

    Google Scholar 

  • Matsuzawa Y, Sarashima M (1986) Intergeneric hybridization between Raphanus sativus L. and Brassica nigra Koch. and alloplasmic radish derivative. Jpn J Breed 36:122–130

    Google Scholar 

  • Matsuzawa Y, Funayama T, Kamibayashi M et al (2000) Synthetic Brassica rapa-Raphanus sativus amphidiploid lines developed by reciprocal hybridization. Plant Breed 119:357–359

    Google Scholar 

  • McCollum GD (1979) Sterility in successive backcrosses of Raphanobrassica (2n = 4x = 36) with recurrent Brassica oleracea (2n = 2x = 18). Can J Genet Cytol 21:479–485

    Google Scholar 

  • McNaughton IH (1973) Synthesis and sterility of Raphanobrassica. Euphytica 22:70–88

    Google Scholar 

  • Meng JL, Shi SW, Li G, Li ZY, Qu XS (1998) The production of yellow-seeded Brassica napus (AACC) through crossing interspecific hybrids of B. campestris (AA) and B. carinata (BBCC) with B. napus. Euphytica 103:329–333

    Google Scholar 

  • Mikkelsen TR, Andersen B, Jørgensen RB (1996) The risk of crop transgene spread. Nature 380:31

    CAS  Google Scholar 

  • Mizushima U (1950) Karyogenetic studies of species and genus hybrids in the tribe Brassiceae of Cruciferae. Tohoku J Agric Res 1:1–14

    Google Scholar 

  • Morinaga T (1931) Interspecific hybridization in Brassica VI. The cytology of F1 hybrids of B. juncea and B. nigra. Cytologia 6:62–67

    Google Scholar 

  • Morinaga T (1933) Interspecific hybridization in Brassica V. The cytology of F1 hybrid of B. carinata and B. alboglabra. Jpn J Bot 6:467–475

    Google Scholar 

  • Morris LE (1936) Pollen germination in Brassica chinensis × Raphanus sativus F1 hybrids. J Genet 33:435–441

    Google Scholar 

  • Moyes CL, Cole SG, Casais CA, Dale PJ (1999) Sexual compatibility between oilseed rape and Sinapis arvensis. In: Proceedings of the 10th international rapeseed congress, Canberra, Australia. Contrib No. 529

  • Moyes CL, Lilley JM, Casais CA et al (2002) Barriers to gene flow from oilseed rape (Brassica napus) into populations of Sinapis arvensis. Mol Ecol 11:103–112

    PubMed  CAS  Google Scholar 

  • Namai H (1980) Effect of flower bud age at bud pollination on cross fertility in reciprocal intergeneric crosses, Raphanus sativus × Brassica oleracea and B. campestris × R. sativus. Cruciferae Newsl 5:26–27

    Google Scholar 

  • Nanda Kumar PBA, Shivanna KR, Prakash S (1988a) Wide hybridization in Brassica. Crossability barriers and studies on the F1 hybrid and synthetic amphidiploid of B. fruticulosa × B. campestris. Sex Plant Reprod 1:234–239

    Google Scholar 

  • Nanda Kumar PBA, Prakash S, Shivanna KR (1988b) Wide hybridisation in crop Brassicas. In: Cresti M, Gori P, Pacini E (eds) Sexual reproduction in higher plants. Springer-Verlag, Berlin, pp 95–100

    Google Scholar 

  • Narain A, Prakash S (1972) Investigations on the artificial synthesis of amphidiploids of Brassica tournefortii Gouan with the other elementary species of Brassica. I. Genomic relationships. Genetica 43:90–97

    Google Scholar 

  • Newstrom LE, Armstrong T, Robertson AW et al (2003) Environmental risks to the New Zealand flora from transgenic crops: the role of gene flow. Landcare Research Report LC0203/065, PO Box 40, Lincoln 7640, New Zealand

  • NRC (2004) Biological confinement of genetically engineered organisms. National Academies Press, Washington

    Google Scholar 

  • OECD (1999) BioTrack database of field trials. Available: http://webdomino1.oecd.org/ehs/biotrack.nsf

  • Olsson G (1960) Species crosses with the genus Brassica I. Artificial Brassica juncea Coss Hereditas 46:171–223

    Article  Google Scholar 

  • Opena RT, Lo SH (1978) Derivation of matroclinal diploids in Chinese cabbage and evaluation of their significance in breeding. J Am Soc Hort Sci 103:820–823

    Google Scholar 

  • Palmer TP (1962) Population structure, breeding system, inter-specific hybridisation and allopolyploidy. Heredity 17:278–293

    Google Scholar 

  • Paulmann W, Röbbelen G (1988) Effective transfer of cytoplasmic male sterility from radish (Raphanus sativus L.) to rape (Brassica napus L.). Plant Breed 100:299–309

    Google Scholar 

  • Pearson OH (1972) Cytoplasmically inherited male sterility characters and flavor components from the species cross Brassica nigra (L) Koch × B. oleracea L J Am Soc Hort Sci 97:397–402

    Google Scholar 

  • Pertl M, Hauser TP, Damgaard C, Jørgensen RB (2002) Male fitness of oilseed rape (Brassica napus), weedy B. rapa and their F1 hybrids when pollinating B. rapa seeds. Heredity 89:212–218

    PubMed  CAS  Google Scholar 

  • Prakash S (1973a) Non-homologous meiotic pairing in the A and B genomes of Brassica; its breeding significance in the production of variable amphidiploids. Genetic Res 21:133–137

    Article  Google Scholar 

  • Prakash S (1973b) Artificial synthesis of Brassica juncea Coss. Genetica 44:249–263

    Google Scholar 

  • Prakash S, Hinata K (1980) Taxonomy, cytogenetics and origin of crop Brassicas, a review. Opera Bot 55:1–57

    Google Scholar 

  • Prakash S, Narain A (1971) Genomic status of Brassica tournefortii Gouan Theor Appl Genet 41:203–204

    Google Scholar 

  • Prakash S, Gupta SK, Raut RN, Kalra AK (1984) Synthetic Brassica carinata – a preliminary report. Cruciferae Newsl 9:36

    Google Scholar 

  • Quiros CF, Ochoa O, Douches DS (1988) Exploring the role of x = 7 species in Brassica evolution: hybridization with B. nigra and B. oleracea. J Heredity 79:351–358

    Google Scholar 

  • R Development Core Team (2005) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available: http://www.R-project.org

  • Rahman L (1976) Inheritance of the contents of erucic, eicosenoic, linolenic, linoleic and oleic acids in crosses between Brassica juncea (L.) Czern & Coss. and B. carinata A. Br Indian J Agric Sci 46:192–198

    CAS  Google Scholar 

  • Rahman L (1978) Relationships between major fatty acids of oleiferous species of Brassica. Indian J Agric Sci 48:401–406

    CAS  Google Scholar 

  • Rahman MH (2001) Production of yellow-seeded Brassica napus through interspecific crosses. Plant Breed 120:463–472

    Google Scholar 

  • Ramanujam S, Srinivasachar D (1943) Cytogenetic investigation in the genus Brassica and the artificial synthesis of B. juncea. Indian J Genet Plant Breed 3:73–90

    Google Scholar 

  • Rao GU, Lakshmikumaran MS, Shivanna KR (1996) Production of hybrids, amphiploids and backcross progenies between a cold-tolerant wild species, Erucastrum abyssinicum and crop brassicas. Theor Appl Genet 92:786–790

    Google Scholar 

  • Rashid A, Rakow G, Downey RK (1994) Development of yellow seeded Brassica napus through interspecific crosses. Plant Breed 112:127–134

    Google Scholar 

  • Raut RN, Kaul T (1982) Synthesis of new genotypes of Brassica napus suitable for cultivation in India. Curr Sci 51:838–839

    Google Scholar 

  • Raybould A, Wilkinson MJ (2005) Assessing the environmental risks of gene flow from GM crops to wild relatives. In: Poppy GM, Wilkinson MJ (eds) Gene flow from GM plants. Blackwell Publishing, Oxford, pp 169–185

    Google Scholar 

  • Richharia RH (1937) Cytological investigation of Raphanus sativus, Brassica oleracea and their F1 and F2 hybrids. J Genet 34:19–44

    Google Scholar 

  • Rieger MA, Preston C, Powles SB (1999) Risks of gene flow from transgenic herbicide-resistant canola (Brassica napus) to weedy relatives in southern Australian cropping systems. Aust J Agric Res 50:115–128

    Google Scholar 

  • Rieger MA, Potter TD, Preston C, Powles SB (2001) Hybridisation between Brassica napus L. and Raphanus raphanistrum L. under agronomic field conditions. Theor Appl Genet 103:555–560

    CAS  Google Scholar 

  • Ringdahl EA, McVetty PBE, Sernyk JL (1987) Intergeneric hybridization of Diplotaxus spp. with Brassica napus: a source of new CMS systems? Can J Plant Sci 67:239–243

    Article  Google Scholar 

  • Robers MB, Williams PH, Osborn TC (1999) Cytogenetics of Eruca sativa/Brassica rapa hybrids produced by embryo rescue. Cruciferae Newsl 21:41–42

    Google Scholar 

  • Roy NN (1980) Species crossability and early generation plant fertility in interspecific crosses of Brassica. Sabrao J 12:43–53

    Google Scholar 

  • Salisbury PA (1991) Genetic variability in Australian wild crucifers and its potential utilisation in oilseed Brassica species. PhD thesis, La Trobe University, Victoria, Australia

  • Salisbury PA (2002) Genetically modified canola in Australia: agronomic and environmental considerations. Australian Oilseeds Federation

  • Sarashima M, Matsuzawa Y (1979) Possibility to breed cabbage lines with the cytoplasm of radish. Cruciferae Newsl 4:32

    Google Scholar 

  • Sarla N, Raut RN (1988) Synthesis of Brassica carinata from Brassica nigra × Brassica oleracea hybrids obtained by ovary culture. Theor Appl Genet 76:846–849

    Google Scholar 

  • Sarla N, Raut RN (1991) Cytogenetical studies on Brassica nigra × B. oleracea hybrids. Indian J Genet Plant Breed 51:408–413

    Google Scholar 

  • Sarla N, Raut R, Shyam P (1987) Synthesis of Brassica carinata A. Br. Curr Sci 56:779–781

    Google Scholar 

  • Sarmah BK, Sarla N (1998) Erucastrum abyssinicum × Brassica oleracea hybrids obtained by ovary and ovule culture. Euphytica 102:37–45

    Google Scholar 

  • Scheffler JA, Dale PJ (1994) Opportunities for gene transfer from transgenic oilseed rape (Brassica napus) to related species. Transgenic Res 3:263–278

    CAS  Google Scholar 

  • Scott SE, Wilkinson MJ (1998) Transgene risk is low. Nature 393:320

    CAS  Google Scholar 

  • Sharma TR, Singh BM (1992) Transfer of resistance to Alternaria brassicae in Brassica juncea through interspecific hybridization among Brassicas. J Genet Breed 46:373–378

    Google Scholar 

  • Sharma DR, Kaur R, Kumar K (1996) Embryo rescue in plants – a review. Euphytica 89:325–337

    Google Scholar 

  • Sikka SM (1940) Cytogenetics of Brassica hybrids and species. J Genet 40:441–509

    Article  Google Scholar 

  • Snogerup S, Persson D (1983) Hybridization between Brassica insularis Moris and B. balearica Pers Hereditas 99:187–190

    PubMed  CAS  Google Scholar 

  • Snow AA, Andow DA, Gepts P et al (2005) Genetically modified organisms and the environment: current status and recommendations. Ecol Appl 15:377–404

    Google Scholar 

  • Sridevi O, Sarla N (1996) Reciprocal hybridization between Sinapis alba and Brassica species. Cruciferae Newsl 18:16

    Google Scholar 

  • Stewart AV (2002) A review of Brassica species, cross-pollination and implications for pure seed production in New Zealand. Agron N Z 32:63–82

    Google Scholar 

  • Struss D, Bellin U, Robbelen G (1991) Development of B-genome chromosome addition lines of B. napus using different interspecific Brassica hybrids. Plant Breed 106:209–214

    Google Scholar 

  • Subudhi PK, Raut RN (1994b) Genetic analysis of yield and its component traits in Indian mustard (Brassica juncea) × Ethiopian mustard (B. carinata) interspecific crosses. Indian J Agric Sci 64:171–175

    Google Scholar 

  • Takeshita M, Kato M, Tokumasu S (1980) Application of ovule culture to the production of intergeneric or interspecific hybrids in Brassica and Raphanus. Jpn J Genet 55:373–387

    Google Scholar 

  • Thalmann C, Guadagnuolo R, Felber F (2001) Search for spontaneous hybridization between oilseed rape (Brassica napus L.) and wild radish (Raphanus raphanistrum L.) in agricultural zones and evaluation of the genetic diversity of the wild species. Bot Helvet 111:107–119

    Google Scholar 

  • U N (1935) Genomic analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn J Bot 7:389–452

    Google Scholar 

  • U N, Nagamatu T, Midusima U (1937) A report on meiosis in the two hybrids, Brassica alba Rabh. (female) × B. oleracea L. (male) and Eruca sativa Lam. (female) × B. oleracea L. (male). Cytologia, Fujii Jubilee Vol, 437–441

  • von Bothmer R, Gustafsson M, Snogerup S (1995) Brassica sect. Brassica (Brassicaceae). II. Inter- and intraspecific crosses with cultivars of B. oleracea. Genet Resour Crop Evol 42:165–178

    Google Scholar 

  • Wahiduzzaman Md (1987) Potentials for species introgression in Brassica napus with special reference to earliness and seed colour. PhD thesis, Swedish University of Agricultural Sciences, Uppsala

  • Warwick SI, Francis A, La Fleche J (2000) Guide to the wild germplasm of brassica and allied crops (tribe Brassiceae, Brassicaceae), 2nd edn. Agriculture and Agri-Food Canada Eastern Cereal and Oilseeds Research Centre, Ottowa. Available: http://www.brassica.info/resources/crucifer_genetics/guidewild.htm

  • Warwick SI, Simard M-J, Légère A et al (2003) Hybridization between transgenic Brassica napus L. and its wild relatives Brassica rapa L., Raphanus raphanistrum L., Sinapis arvensis L., and Erucastrum gallicum (Willd.) O.E. Schulz Theor Appl Genet 107:528–539

    PubMed  CAS  Google Scholar 

  • Wilkinson MJ, Davenport IJ, Charters YM et al (2000) A direct regional scale estimate of transgene movement from genetically modified oilseed rape to its wild progenitors. Mol Ecol 9:983–991

    PubMed  CAS  Google Scholar 

  • Wilkinson MJ, Elliott LJ, Allainguillaume J et al (2003a) Hybridization between Brassica napus and B. rapa on a national scale in the United Kingdom. Science 302:457–459

    PubMed  CAS  Google Scholar 

  • Wilkinson MJ, Sweet J, Poppy GM (2003b) Risk assessment of GM plants: avoiding gridlock? Trends Plant Sci 8:208–212

    PubMed  CAS  Google Scholar 

  • Zhou T, Lu L, Yang G, Al-Shehbaz IA (2001) Brassicaceae (Cruciferae). In: Wu Z, Raven PH (eds) Flora of China, vol 8. Science Press & St Louis, Missouri Botanical Garden Press, Beijing, pp 1–193. Available: http://flora.huh.harvard.edu/china/mss/volume08/BRASSICACEAE.pdf

  • Zhu J, Struss D (1991) Transfer of Phoma lingam resistance from B. nigra into B. napus. Cruciferae Newsl 14/15:16–17

    Google Scholar 

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Acknowledgements

We thank Norman Ellstrand for his advice at the start of this project and encouragement to pursue this research. Thoughtful comments from Christine Bezar, Norman Ellstrand, Peter Heenan and Bill Lee improved the manuscript. This paper is part of the Landcare Research Biosafety Project and the New Zealand Plant BioSafety Database. The funding of both these projects by the New Zealand Foundation for Research, Science and Technology is gratefully acknowledged.

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  1. Tristan T. Armstrong

    Present address: Australian National University, Daley Rd.,, Canberra, ACT 0200, Australia

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  1. Landcare Research, PO Box 40, Lincoln, 7640, Canterbury, New Zealand

    Richard G. FitzJohn, Linda E. Newstrom-Lloyd, Aaron D. Wilton & Michael Cochrane

  2. Landcare Research, Private Bag 92 170, Auckland, 1142, New Zealand

    Tristan T. Armstrong

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FitzJohn, R.G., Armstrong, T.T., Newstrom-Lloyd, L.E. et al. Hybridisation within Brassica and allied genera: evaluation of potential for transgene escape. Euphytica 158, 209–230 (2007). https://doi.org/10.1007/s10681-007-9444-0

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