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Assessment of heterotic potential and combining ability of novel iso-cytoplasmic restorer lines derived from an elite rice hybrid, KRH-2, for the development of superior rice hybrids

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Abstract

Present investigation was carried out to assess the heterotic potential and combining ability of novel iso-cytoplasmic restorer lines [consisting of two recombinant inbred lines (RILs) and two doubled haploid lines] developed from an elite rice hybrid, KRH-2 by crossing them with three popular CMS lines, IR58025A, CRMS32A and APMS6A through line × tester analysis. The doubled haploid line 1 was observed to be a good general combiner for total grain yield per plant (YLD) and other yield component traits and among the CMS lines, IR58025A was observed to be the best combiner as it showed positive significant values for the traits viz., total grain yield per plant, panicle length and spikelet fertility. Higher preponderance of the variance associated with specific combining ability (SCA) as compared to general combining ability (GCA) variance was observed for most of the traits indicated the predominant role of non-additive gene action in the expression of the traits. Out of twelve crosses between the novel iso-cytoplasmic restorers and the CMS lines, 66.6% (eight crosses) showed significant and desirable SCA effects for seven agro-morphological traits. Two crosses IR58025A/RIL-24 and CRMS32A/RIL-24 were observed to be the most promising cross combinations showing standard heterosis of > 50% for YLD trait (as compared with KRH-2) with higher prevalence of GCA and SCA, respectively. The present study also indicates the potentiality of RILs in providing useful parental lines for developing heterotic hybrids which are hard to get from outside sources in the new intellectual property regime.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

ANOVA:

Analysis of variance

BM:

Biomass

BPH:

Better parent heterosis

CV%:

Coefficient of variation in percentage

DFF:

Days to fifty percent flowering (DFF)

DHL:

Doubled haploid lines

FLL:

Flag leaf length

FLW:

Flag leaf width

FGP:

Fertile grains per panicle

GCA:

General combining ability

GCV:

Genotypic coefficient of variance

GP:

Total number of grains per panicle

ICR-Lines:

Iso-cytoplasmic restorer lines

KMR-3R:

Karnataka mandya rice-3R

KRH-2:

Karnataka rice hybrid-2

MPH:

Mid-parent heterosis

NFGPP:

Number of filled grains per panicle

NUFGPP:

Number of unfilled grains per panicle

PCV:

Phenotypic coefficient of variance

PH:

Plant height

PL:

Panicle length

PT:

Productive tillers

PW:

Panicle weight

RCBD:

Randomized complete block design

Rf  genes:

Fertility restoration genes

RIL:

Recombinant inbred line

SCA:

Specific combining ability

SD:

Standard deviation

SE:

Standard error

SFP:

Spikelet fertility in percentage (SFP)

SH:

Standard Heterosis

TGW:

Test (thousand) grain weight

TNGPP:

Total number of grains per panicle

WA-CMS lines:

Wild abortive-cytoplasmic male sterile lines

YLD:

Total grain yield per plant

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Acknowledgements

All the authors are grateful to the Director, ICAR-IIRR, for providing the infrastructural facilities for carrying out this research work.

Funding

This work was supported by DST INSPIRE Fellowship Division, New Delhi, India for providing the financial assistance (Grant # DST/INSPIRE Fellowship/2013/1146, February 2014-March 2019). Swapnil Ravindra Kulkarni has received the support from DST INSPIRE Fellowship Division, New Delhi, India.

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Author notes

  1. Swapnil Ravindra Kulkarni, SM Balachandran, RA Fiyaz and RM Sundaram authors have contributed equally to this work.

Authors and Affiliations

  1. ICAR-Indian Institute of Rice Research (ICAR-IIRR), Hyderabad, 500030, India

    Swapnil Ravindra Kulkarni, S M Balachandran, R A Fiyaz, Divya Balakrishnan, K Sruthi, A S Hari Prasad & R M Sundaram

  2. Centre for Plant Molecular Biology (CPMB), Osmania University, Hyderabad, India

    K Ulaganathan

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  1. Swapnil Ravindra Kulkarni
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  2. S M Balachandran
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  3. R A Fiyaz
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  4. Divya Balakrishnan
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  5. K Sruthi
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  7. A S Hari Prasad
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  8. R M Sundaram
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Contributions

Conceptualization: [FRA, DB, BSM, SRM, SRK] Methodology: [FRA, DB, SK, BSM, SRM]; Formal analysis and investigation: [FRA, SRK, DB, SK]; Writing—original draft preparation: [SRK]; Writing—review and editing: [BSM, FRA, DB, SK, SRM]; Funding acquisition: [SRK, BSM, SRM]; Resources: [UK, HPAS]; Supervision: [BSM, FRA, DB, SK].

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Correspondence to S M Balachandran.

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Kulkarni, S.R., Balachandran, S.M., Fiyaz, R.A. et al. Assessment of heterotic potential and combining ability of novel iso-cytoplasmic restorer lines derived from an elite rice hybrid, KRH-2, for the development of superior rice hybrids. Euphytica 218, 60 (2022). https://doi.org/10.1007/s10681-022-03007-x

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