Abstract
The implementation of molecular tools that help the early selection of genotypes carrying target alleles increases efficiency and reduces the time and costs of breeding programs. The present study aimed the molecular characterization and validation of Single Nucleotide Polymorphism (SNP) targeting disease resistance alleles for assisted selection. A total of 376 common bean lines with contrasting responses for anthracnose and angular leaf spot resistance were used, as well as 149 F2 plants from the cross between BRS Cometa × SEL 1308 (carrying the Anthracnose resistance gene Co-42). Seven of the ten SNP markers evaluated showed potential for assisted breeding: snpPV0025 (Phg-2), snpPV0027 (Phg-5), snpPV0079 (Phg-5), snpPV0046 (Co-u), snpPV0068 (Co-42), snpPV0070 (Co-42) and snpP8282v3-817 (Co-42). Markers snpPV0070 and snpP8282v3-817 showed high efficiency of selection (99.7 and 99.8%, respectively). These markers exhibit great potential to assist in the selection at different stages of the breeding program and may be readily incorporated into marker-assisted selection.
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Acknowledgements
This paper is a cooperative contribution on validation of SNP markers linked to disease resistance genes for their effective use in the routine of Embrapa (Brazilian Agricultural Research Corporation) common bean breeding program. The main goal was to characterize and select elite parent lines according the presence of disease resistance alleles, as well as validate SNP markers for MAS of superior genotypes from populations derived from these parent lines. It reports an elegant example of cross validation and effective use of SNP markers for MAS in an applied breeding program of a very social important orphan crop (common bean or dry bean).
Funding
The authors are grateful to Coordination for the Improvement of Higher Education Personnel (CAPES) for the Grant to Lucas Gomes-Messias (Grant Number: 88882.386267/2019-01) and the National Council for Scientific and Technological Development (CNPq) for the Grants to Rosana Vianello, Helton Pereira, Leonardo Melo and Thiago Souza. This work was supported by the Brazilian Agricultural Research Corporation (EMBRAPA; Grant Number: 02.12.12.005.00.00) and the Research Foundation of the State of Goiás (FAPEG; Grant Number: 201410267001711).
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LG-M, RV, LM and TS planned the study; LG-M, LR and JM-J were responsible for the laboratory analysis; APM developed and phenotyped the F2 population; BR provided the sequence and expertise of the SNP markers; LG-M, HP and TS was responsible for the data analysis; LG-M and RV drafted the manuscript; The final version of the manuscript was revised by all authors.
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Gomes-Messias, L.M., Vianello, R.P., Monteiro-Júnior, J.P. et al. Molecular characterization of parental lines and validation of SNP markers for anthracnose and angular leaf spot in common bean. Euphytica 218, 49 (2022). https://doi.org/10.1007/s10681-022-03002-2
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DOI: https://doi.org/10.1007/s10681-022-03002-2