Abstract
Some species in Vicia sativa complex, also called aggregate (CVS), have economic and ecological advantages and they are frequently used for pasture, silage, and green manure. The main objective of this study was to explore the secondary gene pool for enhancement of common vetch (Vicia sativa L.) germplasm by genome introgressions via conventional crosses made between cultivated common vetch (Vicia sativa L. subspecies sativa) used as female parent with five other subspecies [amphicarpa (L.), Batt., cordata (Wulfen ex Hoppe) Asch. & Graebner, macrocarpa (Moris) Arcang., nigra (L.) Ehrh., and segetalis (Thuill.)], used as male parents. As indicated with very low seed sets, higher levels of sterility were the rule in hybrids in CVS. Hybrids were confirmed using flower petal color and anthocyanin pigmentation markers. Chromosome count studies revealed that certain crossing-experiments were successfully produced interspecific hybrids. Certain offspring progenies in further generations were also obtained. Our studies clearly indicated that complex CVS and hybrids obtained from these species consisted of 10, 11, 12, 13 and 14 pair of somatic chromosomes. Results indicated that there exist significant incompatibility alleles possibly expressing in the zygote or post-zygotic developmental stages within the CVS. In conclusion, present study revealed that sufficient amount of seeds could be obtained from the crosses in the CVS and this stimulates renewed interest in utilizing the secondary gene pool as a source of genetic variation in breeding programs of common vetch.
Similar content being viewed by others
References
Abd El-Moneim AM (1993) Selection for non-shattering common vetch, Vicia sativa L. Plant Breed 110:168–171
Abdalla MM (1977) Intraspecific unilateral incompatibility in Vicia faba L. Theor Appl Genet 50:227–233
Acikgoz E (1982a) Cold tolerance and its association with seedling morphology and chemical composition in annual forage legumes. II. Vetch (Vicia) species. Plant Breed 88:278–286
Acikgoz E (1982b) Parameters of cold tolerance in common vetch. Euphytica 31:997–1001
Acikgoz E (1984) Herbage and seed yield of subterranean vetch in response to cutting treatments. J Agron Crop Sci 153:260–263
Acikgoz E (1986) Annual forage legumes in the arid and semi-arid regions of Turkey. In: Beck DP, Materon LA (eds) Nitrogen fixation by legumes in Mediterranean agriculture. Martinus Nijhoff Publ, Leiden, pp 47–54. https://doi.org/10.1007/978-94-009-1387-5_6
Ahmed S, Akem C, Abd El Moneim AM (2000) Sources of resistance to downy mildew in narbon (Vicia narbonensis) and common (Vicia sativa) vetches. Genet Resour Crop Evol 47:153–156
Arslan E, Ertugrul K, Ozturk AB (2012) Karyological studies of some species of the genus Vicia L. (Leguminosae) in Turkey. Caryologia 65:106–113
Cakmakci S, Aydinoglu B, Karaca M (2003) Determining relationships among yield and yield components using correlation and path coefficient analyses in summer sown common vetch (Vicia sativa L.) genotypes. Pak J Bot 35:387–400
Cakmakci S, Aydinoglu B, Karaca M (2006) Heritability of yield components in common vetch (Vicia sativa L.). Acta Agric Scand, Sect B-Soil Plant Sci 56(1):54–59. https://doi.org/10.1080/09064710510008531
Celiktas N, Can E, Hatipoglu R, Avci S (2006) Comparison between a wild population and cultivar of common vetch (Vicia sativa L., Fabaceae) on cytological and agronomic characteristics. N Z J Agric Res 49:389–393
Chai X, Dong R, Liu W, Wang Y, Liu Z (2017) Optimizing sample size to assess the genetic diversity in common vetch (Vicia sativa L.) populations using start codon targeted (SCoT) markers. Molecules 22:567. https://doi.org/10.3390/molecules22040567
Chowdhury DMS, Rathjen JM, Tate ME, McDonald D (2004) Genetics of colour traits in common vetch (Vicia sativa L.). Euphytica 136:249–255
Chung JW, Kim TS, Suresh S, Lee SY, Cho GT (2013) Development of 65 novel polymorphic cDNA-SSR markers in Common Vetch (Vicia sativa subsp. sativa) using next generation sequencing. Molecules 18:8376–8392. https://doi.org/10.3390/molecules18078376
Cooper RL (1958) Hybridization in vetch. Thesis, Michigan State University
Cubero I (1982) Interspecific hybridization in Vicia. In: Hawtin G, Webb C (eds) Faba bean improvement, proceedings of the faba bean conference. Martinus Nijhoff Publ, Leiden, pp 91–108
Davis PH, Plitmann U (1970) Vicia L. In: Davis PH (ed) Flora of Turkey. The University Press, Edinburgh, pp 274–325
Dong R, Dong D, Luo D, Zhou Q, Chai X, Zhang J, Xie W, Liu W, Dong Y, Wang Y, Liu Z (2017) Transcriptome analyses reveal candidate pod shattering-associated genes involved in the pod ventral sutures of common vetch (Vicia sativa L.). Front Plant Sci 8:649. https://doi.org/10.3389/fpls.2017.00649
Donnelly ED (1958) Inheritance of white flower color in common vetch, Vicia sativa. Agron J 50:763–764
Donnelly ED, Clark EM (1962) Hybridization of the genus Vicia. Crop Sci 2:141–145
El-Bok S, Zoghlami-Khelil A, Brahim TB, Ouji A, Hassen H, Lamine O, Jabri C, Douggari R, El-Gazzah M (2014) Chromosome number and karyotype analysis of some taxa of Vicia genus (Fabaceae): revision and description. Int J Agric Biol 16:1067–1074
El-Bok S, Zoghlami-Khelil A, Dougari R, Jabri C, Lamine O, El-Gazzah M (2015) Vicia sativa subsp. sativa (Fabaceae): new taxonomic division in Tunisia based on karyological data. Pak J Agric Sci 52:279–283
El-Shanshoury AR (2007) Characterization of infraspecific electrophoretic genetic variation within Vicia sativa subspecies sativa population. J Biol Sci 7:918–924
Enneking D, Wink M (2000) Towards the elimination of antinutritional factors in grain legumes. In: Knight R (ed) Linking research and marketing opportunities for pulses in the 21st century, current plant science and biotechnology in agriculture. Kluwer Academic Publishers, Dordrecht, pp 375–384
Firincioglu HK, Tate M, Unal S, Dogruyol L, Ozcan I (2007) A selection strategy for low toxin vetches (Vicia sativa spp.). Turk J Agric For 31:303–311
Frediani M, Maggini F, Gelati MT, Cremonini R (2004) Repetitive DNA sequences as probes for phylogenetic analysis in Vicia genus. Caryologia 57:379–386
Geogieva N (2018) Suitability of vetch (Vicia sativa L. and V. villosa Roth) cultivars for organic farming conditions. Pak J Bot 50:161–167
Gil J, Cubero JI (1993) Multivariate analysis of the Vicia sativa L. aggregate. Bot J Linn Soc 113:389–400
Haider AS, El-Shanshoury AR (2000) Variability of storage proteins and esterase isozymes in Vicia sativa subspecies. Biol Plant 43:205–209
Han Y, Liu Y, Wang H, Liu X (2017) The evolution of Vicia ramuliflora (Fabaceae) at tetraploid and diploid levels revealed with FISH and RAPD. PLoS ONE 12(1):e0170695. https://doi.org/10.1371/journal.pone.0170695
Hanelt P, Mettin D (1989) Biosystematics of the genus Vicia L. (Leguminosae). Annu Rev Ecol Evol Syst 20:199–223
Hollings E, Stace CA (1974) Karyotype variation and evolution in the Vicia sativa aggregate. New Phytol 73:195–208
Hollings E, Stace CA (1978) Morphological variation in the Vicia sativa L. aggregate. Watsonia 12:1–14
Hollings E, Stace CA (2006) Karyotype variation and evolution in the Vicia sativa L. aggregate. New Phytol 73:195–208
Hueze V, Tran G, Baumont R (2011) Common vetch (Vicia sativa). Feedipedia 12:53–62
Ince AG, Karaca M, Onus AN (2010) A reliable gender diagnostic PCR assay for jojoba (Simmondsia chinensis (Link) Schneider). Genet Resour Crop Evol 57:773. https://doi.org/10.1007/s10722-009-9516-1
Karaca M, Ince AG. (2018a). Identification of heterotic group for genetic and epigenetic studies in maize. In: Proceedings og international congress on agriculture and animal science, 7–9 November, Alanya, Turkey, pp 546–552
Karaca M, Ince AG (2018b) New generation plant breeding methods (Molecular Plant Breeding) some advantages & disadvantage. Res J Agric Sci 11:39–49
Karere GM, Lyons LA, Froenicke L (2010) Enhancing radiation hybrid mapping through whole genome amplification. Hereditas 147:103–112. https://doi.org/10.1111/j.1601-5223.2010.02166.x
Kim TS, Raveendar S, Suresh S, Lee GA, Lee JR, Cho JH, Lee SY, Ma KH, Cho GT, Chung JW (2015) Transcriptome analysis of two Vicia sativa subspecies: mining molecular markers to enhance genomic resources for vetch improvement. Genes 6:1164–1182. https://doi.org/10.3390/genes6041164
Ladizinsky G (1978) Chromosomal polymorphism in wild populations of Vicia sativa. Caryologia 31:233–241
Ladizinsky G (1981) Consequences of hybridization in Vicia sativa aggregate. Heredity 47:431–438
Ladizinsky G (2014) Chromosomal polymorphism in wild populations of Vicia sativa L. Caryologia 31:233–241
Ladizinsky G, Shefer Y (1982) Polyploidy in the Vicia sativa aggregate. New Phytol 91:541–547
Ladizinsky G, Temkin R (1978) The cytogenetic structure of Vicia sativa aggregate. Theor Appl Genet 53:33–42
Martin E, Yildiz HK, Kahraman A, Binzat OK, Eroglu HE (2018) Detailed chromosome measurements and karyotype asymmetry of some Vicia (Fabaceae) taxa from Turkey. Caryologia 71:224–232. https://doi.org/10.1080/00087114.2018.1460058
Maxted N (1993) A phenetic investigation of Vicia L. subgenus Vicia (Leguminosae–Vicieae). Bot J Linn Soc 111:155–182
Mettin D, Hanelt P (1964) Cytosystematische untersuchungen in der artengruppe urn Vicia sativa L. Kulturpflanze 12:163–225
Mikic A, Mihailovic V, Cupina B, Milic D, Katic S, Karagic D, Pataki I, D’Ottavio P, Kraljevic-Balalic M (2014) Forage yield components and classification of common vetch (Vicia sativa L.) cultivars of diverse geographic origin. Grass Forage Sci 69:315–322
Navratilova A, Neumann P, Macas J (2003) Karyotype analysis of four Vicia species using in situ hybridization with repetitive sequences. Ann Bot 91:921–926
Potokina EK (1997) Vicia sativa L. aggregate (Fabaceae) in the flora of the former USSR. Genet Resour Crop Evol 44:199–209
Potokina E, Tomooka N, Vaughan DA, Alexandrova T, Xu R-Q (1999) Phylogeny of Vicia subgenus Vicia (Fabaceae) based on analysis of RAPDs and RFLP of PCR-amplified chloroplast genes. Genet Resour Crop Evol 46:149–161
Potokina E, Blattner F, Alexandrova T, Bachmann K (2002) AFLP diversity in the common vetch (Vicia sativa L.) on the world scale. Theor Appl Genet 105:58–67
Raveendar S, Lee GA, Jeon YA, Lee YJ, Lee JR, Cho GT, Cho JH, Park JH, Ma KH, Chung JW (2015) Cross-amplification of Vicia sativa subsp. sativa microsatellites across 22 other Vicia species. Molecules 20:1543–1550. https://doi.org/10.3390/molecules20011543
Rousi A (1961) Cytotaxonomical studies on Vicia cracca L. and V. tenuifolia Roth. I. Chromosome number and karyotype evolution. Hereditas 47:81–110
Schifino-Wittmann MT (2000) The cytogenetics and evolution of forage legumes from Rio Grande do Sul: a review. Genet Mol Biol 23:989–995
Seymour M, Siddique KHM, Brandon N, Martin L, Jackson E (2002) Response of vetch (Vicia spp.) to plant density in south-western Australia. Aust J Exp Agric 42:1043–1051
Shiran B, Raina SN (2001) Evidence of rapid evolution and incipient speciation in Vicia sativa species complex based on nuclear and organellar RFLPs and PCR analysis. Genet Resour Crop Evol 5:519–532
Tate M, Ennenking D (2006) Common vetch (Vicia sativa ssp. sativa): feed or future food. Grain Legumes 47:16–17
Tiryaki GY, Cil A, Tiryaki I (2016) Revealing seed coat colour variation and their possible association with seed yield parameters in common vetch (Vicia sativa L.). Int J Agron. https://doi.org/10.1155/2016/1804108
Trognitz BR (1991) Comparison of different pollen viability assays to evaluate pollen fertility of potato dihaploids. Euphytica 56:143–148
Uzun A, Gucer S, Acikgoz E (2011) Common vetch (Vicia sativa L.) germplasm: correlations of crude protein and mineral content to seed traits. Plant Foods Hum Nutr 66:254–260
Van de Wouw M, Maxted N, Chabane K, Ford-Lloyd BV (2001) Molecular taxonomy of Vicia ser. Vicia based on amplified fragment length polymorphisms. Plant Syst Evol 229:99–105
Van de Wouw M, Maxted N, Chabane K, Ford-Lloyd BV (2003) A multivariate and cladistic study of Vicia L. ser. Vicia (Fabaceae) based on analysis of morphological characters. Plant Syst Evol 237:19–39
Watanabe K, Yamada T (1958) Studies on the interspecific hybridization of common vetch Vicia sativa, and Yahazuendo, V. angustifolia var. segetalis. Bull Natl Inst Agric Sci Jpn Ser G 15:109–145
Yamamoto K (1968) On the interspecific hybrids between Vicia sativa and V. macrocarpa. Jpn J Breed 18:283–290
Yamamoto K (1974) Hybrid plants between two races of Vicia amphicarpa having 2n = 10 and 2n = 14 chromosomes. Jpn J Breed 24:73–80
Zohary D, Plitmann U (1979) Chromosome polymorphism, hybridization and colonization in the Vicia sativa group (Fabaceae). Plant Syst Evol 31:143–156
Acknowledgements
This study was financially supported in part by the Scientific and Technological Research Council of Turkey (Project No: 214O224).
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Kartal, G.K., Senbek, G., Karaca, M. et al. Hybridization studies in Vicia sativa complex. Euphytica 216, 29 (2020). https://doi.org/10.1007/s10681-020-2566-3
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s10681-020-2566-3