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Underlying genetic variation in the response of cultivated and wild soybean to enhanced ultraviolet-B radiation

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Abstract

Reductions in stratospheric ozone have resulted in increased levels of ultraviolet-B (UV-B, 280–315 nm) radiation reaching the earth’s surface. UV-B radiation can damage plant DNA and photosynthetic machinery. Therefore, enhanced UV-B levels would seriously affect crop production in several regions of the world. Here, we provide a comprehensive report of intraspecific variability in the response of soybean to UV-B radiation and the genetic variation underlying UV-B resistance. We evaluated 140 genotypes, including 94 Glycine max and 46 G. soja accessions, for their sensitivity to supplemental UV-B radiation. Differences in responses to elevated UV-B levels, such as changes in leaf area and aerial biomass, were observed among soybean genotypes, supporting the notion that there is intraspecific variability in this response in G. soja as well as G. max. To explore how genetic variation contributes to differences in UV-B resistance, we sequenced the most UV-B resistant (IT162669) and sensitive (Cheongjakong 3) genotypes at the whole-genome level. Based on their homology and functional annotation, 137 genes were determined to be “UV-B-related genes” in soybean, harboring almost 100 high-confidence single nucleotide polymorphisms between the two genotypes. Interestingly, we identified four genes with non-synonymous mutations that are related to plant protection mechanisms, such as the UV-protection, DNA damage repair and DNA damage tolerance pathways. Our results provide valuable information about UV-B-resistant soybean genotypes, UV-B-related soybean genes, and sequence variations between resistant and sensitive genotypes.

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Acknowledgments

This study was supported by a grant from the Next Generation BioGreen 21 Program (No. PJ008060) of the Rural Development Administration, Republic of Korea. Suk-Ha Lee and Moon-Young Kim thank International Atomic Energy Agency (Research Contract No. 15664) and National Research Foundation of Korea (2012R1A1A3014252), respectively.

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  1. Kyung Do Kim

    Present address: Center for Applied Genetic Technologies, University of Georgia, 111 Riverbend Rd., Athens, GA, 30602, USA

Authors and Affiliations

  1. Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea

    Kyung Do Kim, Min Young Yun, Jin Hee Shin, Yang Jae Kang, Moon Young Kim & Suk-Ha Lee

  2. Plant Genomic and Breeding Research Institute, Seoul National University, Seoul, 151-921, Korea

    Suk-Ha Lee

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  1. Kyung Do Kim
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Kim, K.D., Yun, M.Y., Shin, J.H. et al. Underlying genetic variation in the response of cultivated and wild soybean to enhanced ultraviolet-B radiation. Euphytica 202, 207–217 (2015). https://doi.org/10.1007/s10681-014-1271-5

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