Skip to main content
SpringerLink
Log in

Marker-aided breeding for resistance to bean common mosaic virus in Kyrgyz bean cultivars

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Common bean (Phaseolus vulgaris L.) is an important export crop in Kyrgyzstan. Bean common mosaic virus (BCMV) is a seed-born disease that affects this crop and reduces its yield significantly in the country. The aim of this study was to identify virus strain(s) occurring in Kyrgyzstan and breed host plant resistance to BCMV using DNA markers. Susceptible Kyrgyz cultivars (Ryabaya, Kytayanka and Lopatka) were included in a backcrossing breeding scheme for introducing host plant resistance from resistant cultivars (Vaillant and Flagrano). The virus strains were evaluated according to the symptoms of differential cultivars. The virus strain NL6 was found in northern Kyrgyzstan, where farmers grow most of the common bean produced in this country. Two SCAR markers (SW13 and SBD5) were used successfully in marker-aided backcrossing for pyramiding the I and bc-1 2 genes, which provide host plant resistance to BCMV. Resistant BC4F2 offspring carrying the I gene showed hypersensitivity reactions to necrosis inducing NL3 strain after detached leaf-assays.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Bekele E, Geleta M, Dagne K, Jones A, Barnes I, Bradman N, Thomas M (2007) Molecular phylogeny of genus Guizotia (Asteraceae) using DNA sequences derived from ITS. Gen Res Crop Evol 54:1419–1427

    Article  CAS  Google Scholar 

  • Burke DW, Silbernagel MJ, Kraft JM, Koehler HH (1995) Registration of ‘Othello’ pinto bean. Crop Sci 35:943

    Google Scholar 

  • CIA (2011) CIA yearbook 2011. Central Intelligence Agency, Washington DC

    Google Scholar 

  • Drijfhout E (1978) Genetic interaction between Phaseolus vulgaris L. and bean common mosaic virus with implication for strain identification and breeding for resistance. Verslagen van Landbouwkundige Onderzoekingen, Wageningen

  • Drijfhout E, Bos L (1977) Identification of two new strains of bean common mosaic virus. Neth J Plant Pathol 83:13–25

    Article  Google Scholar 

  • Drijfhout E, Silbernagel MJ, Burke DW (1978) Differentiation of strains of bean common mosaic virus. Neth J Plant Pathol 84:13–26

    Article  Google Scholar 

  • FAO (2010) FAOSTAT. Food and Agriculture Organization of the United Nations, Rome, Italy. http://www.fao.org/. Accessed 10 May 2012

  • FAO (2012) Country profile Kyrgyzstan. Food and Agriculture Organization of the United Nations, Rome, Italy. http://www.fao.org/. Accessed 9 Mar 2013

  • Flores-Estevez N, Acosta-Gallegos JA, Silva-Rosales L (2003) Bean common mosaic virus and bean common mosaic necrosis virus in Mexico. Plant Dis 87:21–25

    Article  Google Scholar 

  • Galvez GE, Morales FJ (1989) Aphid-transmitted viruses. In: Schwartz HF, Pastor-Corrales MA (eds) Bean production problems in the Tropics, 2nd edn. International Center for Tropical Agriculture CIAT, Cali, pp 333–361

    Google Scholar 

  • Guzman P, Rojas MR, Davis RM, Kimble K, Stewart R, Sundstrom FJ, Gilbertson RL (1997) First report of bean common mosaic necrosis potyvirus (BCMNV) infecting common bean in California. Plant Dis 81:831

    Article  Google Scholar 

  • Ha C, Revill P, Harding RM, Vu M, Dale JL (2008) Identification and sequence analysis of potyviruses infecting crops in Vietnam. Arch Virol 153:45–60

    Article  PubMed  CAS  Google Scholar 

  • Haley SD, Afanador L, Kelly JD (1994) Identification and application of a random amplified polymorphic DNA marker for the I gene (potyvirus resistance) in common bean. Phytopathogy 84:157–160

    Article  CAS  Google Scholar 

  • Hampton RO (1975) The nature of bean yield reduction by bean yellow and bean common mosaic viruses. Phytopathology 65:1342–1346

    Article  Google Scholar 

  • Hegay S, Asanaliev A, Torutaeva E, Hede A, Islamov A, Baltabaev E, Marx E (2008) Screening local Phaseolus vulgaris populations. Vestn KNAU 11:159–163

    Google Scholar 

  • Hosfield GL, Varner GV, Uebersax MA, Kelly JD (2004) Registration of ‘Merlot’ small red bean. Crop Sci 44:351–352

    Article  Google Scholar 

  • JICA (2010) Common bean production in Kyrgyzstan. Bishkek, Japan International Cooperation Agency

    Google Scholar 

  • Johnson WC, Guzman P, Mandala D, Mkandawire ABC, Temple S, Gilbertson RL, Gepts P (1997) Molecular tagging of the bc-3 gene for introgression into Andean common bean. Crop Sci 37:248–254

    Article  CAS  Google Scholar 

  • Kapil R, Sharma P, Sharma SK, Sharma OP, Dhar JB, Sharma PN (2011) Pathogenic and molecular variability in bean common mosaic virus infecting common bean in India. Arch Phytopathol Plant Prot 44:1081–1092

    Article  CAS  Google Scholar 

  • Kelly JD (1997) The review of varietal response to bean common mosaic potyvirus in Phaseolus vulgaris. Plant Var Seeds 10:1–6

    Google Scholar 

  • Kelly JD, Saettler AW, Morales M (1983) New necrotic strain of bean common mosaic virus in Michigan. Annu Rep Bean Improv Coop 26:49–50

    Google Scholar 

  • Kelly JD, Hosfield GL, Varner GV, Uebersax MA, Haley SD, Taylor J (1994) Registration of ‘Raven’ black bean. Crop Sci 34:1406–1407

    Article  Google Scholar 

  • Kelly JD, Afanador L, Haley SD (1995) Pyramiding genes for resistance to bean common mosaic virus. Euphytica 82:207–212

    Article  Google Scholar 

  • Kelly JD, Hosfield GL, Varner GV, Uebersax MA, Long RA, Taylor J (1998) Registration of ‘Red Hawk’ dark red kidney bean. Crop Sci 38:280–281

    Google Scholar 

  • Kelly JD, Varner GV, Hosfield GL, Uebersax MA, Taylor J (2006) Registration of ‘Sedona’ pink bean. Crop Sci 46:2707–2708

    Article  Google Scholar 

  • McKern NM, Mink GI, Barnett OW, Mishra A, Whittaker LA, Silbernagel MJ, Ward CW, Shukla DD (1992) Isolates of bean common mosaic virus comprising two distinct potyviruses. Phytopathol 82:923–929

    Article  CAS  Google Scholar 

  • Melotto M, Afanador L, Kelly JD (1996) Development of a SCAR marker linked to the I gene in common bean. Genome 39:1216–1219

    Article  PubMed  CAS  Google Scholar 

  • Miklas P, Lambert S, Mink G, Silbernagel M (1998) Many beans with bc-3 resistance to BCMNV are susceptible to BCMV. Annu Rep Bean Improv Coop 41:33–34

    Google Scholar 

  • Miklas PN, Larsen RC, Riley R, Kelly JD (2000) Potential marker-assisted selection for bc-1(2) resistance to bean common mosaic potyvirus in common bean. Euphytica 116:211–219

    Article  CAS  Google Scholar 

  • Miklas PN, Hang AN, Kelly JD, Strausbaugh CA, Forster RL (2002) Registration of three kidney bean germplasm lines resistant to bean common mosaic and necrosis potyviruses: USLK-2 light red kidney, USDK-4 dark red kidney, and USWK-6 white kidney. Crop Sci 42:674–675

    Article  Google Scholar 

  • Morales FJ, Castano M (1987) Seed transmission characteristics of selected bean common mosaic virus strains in differential bean cultivars. Plant Dis 71:51–53

    Article  Google Scholar 

  • Mukeshimana G, Hart LP, Kelly JD (2003) Bean common mosaic virus and bean common mosaic necrosis virus. Extension Bulletin E-2894 (Major Rev. of E-1561). Michigan State University extension. http://fieldcrop.msu.edu/uploads/documents/E2894.pdf. Accessed 20 Feb 2013

  • Mukeshimana G, Paneda A, Rodriguez-Suarez C, Ferreira JJ, Giraldez R, Kelly JD (2005) Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean. Euphytica 144:291–299

    Article  Google Scholar 

  • Naderpour M, Mohammadi M, Mossahebi GH, Koohi-Habibi M (2010) Identification of three strains of bean common mosaic necrosis virus in common bean from Iran. Plant Dis 94:127

    Article  Google Scholar 

  • Ortiz R (2012) Marker-aided breeding revolutionizes twenty-first century crop improvement. In: Agrawal GK, Rakwal R (eds) Seed development: OMICS technologies toward improvement of seed quality and crop yield. Springer, The Netherlands, pp 435–452

    Chapter  Google Scholar 

  • Pastor-Corrales MA, Kelly JD, Steadman JR, Lindgren DT, Stavely JR, Coyne DP (2007) Registration of six great Northern bean germplasm lines with enhanced resistance to rust and bean common mosaic and necrosis potyviruses. J Plant Regist 1:77–79

    Article  Google Scholar 

  • Schmidt HE, Reichnbacher D, Lehmann C, Verderevskaja TD, Basky Z, Horvath J (1987) Identification of pathotypes of bean common mosaic virus occurring in the German Democratic Republic, in the Soviet Union and in the Hungarian Peoples Republic. Arch Phytopathol Plant Prot 23:105–115

    Article  Google Scholar 

  • Sharma PN, Pathania A, Kapil R, Sharma P, Sharma OP, Patial M, Kapoor V (2008) Resistance to bean common mosaic potyvirus strains and its inheritance in some Indian land races of common bean. Euphytica 164:173–180

    Article  CAS  Google Scholar 

  • Silbernagel MJ (1994) Release of pinto breeding line 92US-1006 with I bc2 2 resistance to bean common mosaic virus. Annu Rep Bean Improv Coop 37:246

    Google Scholar 

  • Spence NJ, Walkey DGA (1995) Variation for pathogenicity among isolates of bean common mosaic virus in Africa and a reinterpretation of the genetic relationship between cultivars of Phaseolus vulgaris and pathotypes of BCMV. Plant Path 44:527–546

    Article  Google Scholar 

  • Strausbaugh CA, Myers JR, Forster RL, McClean PE (1999) Bc-1 and bc-u—two loci controlling bean common mosaic virus resistance in common bean are linked. J Am So Hort Sci 124:644–648

    Google Scholar 

  • Temple S, Smithson JB (1989) The crossing of beans. In: Arregoces O, Medina LM (eds) CIAT study guide. International Center for Tropical Agriculture, Cali

    Google Scholar 

  • Tu JC (1986) Isolation and characterization of a new necrotic strain (NL-8) of bean common mosaic virus in southwestern Ontario. Can Plant Dis Surv 66:13–14

    Google Scholar 

  • Vandemark GJ, Miklas PN (2002) A fluorescent PCR assay for the codominant interpretation of a dominant SCAR marker linked to the virus resistance allele bc-1(2) in common bean. Mol Breeding 10:193–201

    Article  CAS  Google Scholar 

  • Vandemark GJ, Miklas PN (2005) Genotyping common bean for the potyvirus resistance alleles I and bc-1(2) with a multiplex real-time polymerase chain reaction assay. Phytopathology 95:499–505

    Article  PubMed  CAS  Google Scholar 

  • Vetten HJ, Lesemann DE, Maiss E (1992) Serotype A and B strains of bean common mosaic virus are two distinct potyviruses. Arch Virol 5:415–431

    Article  CAS  Google Scholar 

  • Wood DR, Keenan JG (1982) Registration of Olathe bean. Crop Sci 22:1259–1260

    Article  Google Scholar 

  • Wortmann CS, Kirkby RA, Eledu CA, Allen DJ (1998) Atlas of common bean (Phaseolus vulgaris L.) production in Africa. CIAT, Cali

Download references

Acknowledgments

This research was undertaken under the PhD study of the first author. We are thankful for the funding provided by the Swedish International Development Agency (Sida). We also acknowledge with thanks Ann-Charlotte Strömdahl and Ann-Sofie Fält for their assistance in the laboratory. Special thanks to Drs. Elie Marx (INRA, France) and James D. Kelly (Michigan State University, East Lansing, MI, USA) for their guidance regarding breeding for host plant resistance to BCMV and for providing some of the common bean germplasm used in this study.

Author information

Authors and Affiliations

  1. Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, P. O. Box 101, 23053, Alnarp, Sweden

    Sergey Hegay, Rodomiro Ortiz, Larisa Garkava-Gustavsson, Helena Persson Hovmalm & Mulatu Geleta

  2. Department of Agronomy, Kyrgyz National Agrarian University, Mederova 68, 720005, Bishkek, Kyrgyz Republic

    Sergey Hegay

Authors
  1. Sergey Hegay
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rodomiro Ortiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Larisa Garkava-Gustavsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Helena Persson Hovmalm
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mulatu Geleta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sergey Hegay.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hegay, S., Ortiz, R., Garkava-Gustavsson, L. et al. Marker-aided breeding for resistance to bean common mosaic virus in Kyrgyz bean cultivars. Euphytica 193, 67–78 (2013). https://doi.org/10.1007/s10681-013-0928-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-013-0928-9

Keywords

Search

Navigation