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Development of a chromosome segment substitution line population with wild soybean (Glycine soja Sieb. et Zucc.) as donor parent

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Abstract

Chromosome segment substitution line (CSSL) population is potential in precisely detecting and pyramiding genes/QTL/segments due to the genetic background noise removed. To exploit and utilize the favorable wild alleles, a CSSL population with 151 lines (SojaCSSLP1) was generated using a wild soybean (Glycine soja Sieb. et Zucc.) N24852 as donor parent and the elite cultivar NN1138-2 as its genetic background. An improved CSSL construction strategy was used, i.e. continuous backcross after initial crossing followed with alternation of backcross and selfing combined with marker-assisted selection based on pedigree DNA pools and phenotypic differences among pedigrees. The SojaCSSLP1 with an average recovery ratio of 95.7 % of the NN1138-2 genome could cover the entire genome of wild soybean. Four wild alleles/segments for each of the two wild characteristics, longer plant height (PH) and more number of nodes on main stem (NN), in a total of six segments, were detected with additive effects all positive. Among them, Satt243 on Chr.10 and Sat_286 on Chr.19 associated with both PH and NN while Satt338 and SOYGPATR on Chr.4 and Satt314 neighboring with Satt192 on Chr.12 had the former and latter on each chromosome associated with PH and NN, respectively. That could explain the high positive correlation between the two traits (r = 0.88). Compared with those in the literature, three QTL/segments for PH and one for NN were detected also among cultivated soybeans, indicating allele differentiation happened not only between wild and cultivated but also among cultivated soybeans. Therefore these QTL/segments might be the key ones to explain the domestication and evolution of soybean. In addition, SojaCSSLP1 should be also potential in studies for multiple wild traits due to its broad variation.

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Acknowledgments

We would thank all the participants in the research for their assistance, advice and encouragement. This work was supported by the National Key Basic Research Program of China (2009CB1184, 2010CB1259, 2011CB1093), the National Hightech R&D Program of China (2011AA10A105, 2012AA101106), the Natural Science Foundation of China (31071442), the MOA Public Profit Program (200803060) and the MOE 111 Project (B08025).

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  1. Soybean Research Institute/National Center for Soybean Improvement/MOA Key Laboratory for Biology and Genetic Improvement of Soybean (General)/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Wubin Wang, Qingyuan He, Hongyan Yang, Shihua Xiang, Tuanjie Zhao & Junyi Gai

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  1. Wubin Wang
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  2. Qingyuan He
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Correspondence to Junyi Gai.

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Wang, W., He, Q., Yang, H. et al. Development of a chromosome segment substitution line population with wild soybean (Glycine soja Sieb. et Zucc.) as donor parent. Euphytica 189, 293–307 (2013). https://doi.org/10.1007/s10681-012-0817-7

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