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Genomic in situ hybridization (GISH) analysis of intergeneric hybrids in Colchicaceae

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Abstract

Some intergeneric hybrids produced by the crosses among several colchicaceous ornamentals, Gloriosa superba ‘Lutea’ (2n = 2x = 22), G. ‘Marron Gold’ (2n = 4x = 44), G. ‘Verschild’ (2n = 7x = 77), Littonia modesta Hook. (2n = 2x = 22), and Sandersonia aurantiaca Hook. (2n = 2x = 24), were subjected to genomic in situ hybridization (GISH) analysis in order to clarify their genome constitutions. Chromosome preparation was made from root tip cells of L. modesta × G. superba ‘Lutea’, L. modesta × S. aurantiaca, S. aurantiaca × G. superba ‘Lutea’, L. modesta × G. ‘Marron Gold’, S. aurantiaca × G. ‘Marron Gold’ and G. ‘Verschild’ × S. aurantiaca. Total DNA of one parent was labeled with digoxigenin or biotin and used as probe, and chromosomes were counterstained with 4′-6-diamidono-2-phenylindole (DAPI). For all the nine intergeneric hybrids, chromosomes from each parent could be clearly distinguished by GISH analysis. Thus GISH analysis is a powerful tool for identifying the genome constitution of intergeneric hybrids in colchicaceous ornamentals. The results obtained by GISH analysis study may be important for further progress in breeding of colchicaceous ornamentals.

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Acknowledgments

This work was supported in part by a Grant-in-Aid for Scientific Research (No. 20580023) from the Ministry of Education, Culture, Sports, Science and Technology, Japan. We are grateful to Mr. Shiro Mizogami, Keyence Corp., Japan, for his valuable suggestions.

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Authors and Affiliations

  1. Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, Niigata, 950-2181, Japan

    Daisuke Nakazawa, Tomonari Kishimoto, Takeru Sato, Tomoka Saito, Junji Amano, Sachiko Kuwayama, Yusuke Watanabe, Dong-Sheng Han & Masaru Nakano

  2. 3250-201 Fukaisawa-machi, Naka-ku, Sakai, 599-8236, Japan

    Hajime Okuno

  3. Botanic Gardens of Toyama, 42 Kamikutsuwada, Fuchu-machi, Toyama, 939-2713, Japan

    Toshinari Godo

  4. Niigata Agricultural Research Institute, Horticultural Research Center, 177 Mano, Seiro, Niigata, 957-0111, Japan

    Yusuke Watanabe

Authors
  1. Daisuke Nakazawa
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  2. Tomonari Kishimoto
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  3. Takeru Sato
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  4. Tomoka Saito
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  5. Junji Amano
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  6. Sachiko Kuwayama
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  7. Hajime Okuno
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  8. Toshinari Godo
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  9. Yusuke Watanabe
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  10. Dong-Sheng Han
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  11. Masaru Nakano
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Correspondence to Masaru Nakano.

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Nakazawa, D., Kishimoto, T., Sato, T. et al. Genomic in situ hybridization (GISH) analysis of intergeneric hybrids in Colchicaceae. Euphytica 181, 197–202 (2011). https://doi.org/10.1007/s10681-011-0393-2

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  • DOI: https://doi.org/10.1007/s10681-011-0393-2

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