Abstract
We established a new auxiliary phylogenetic approach based on genomic in situ hybridization technique (GISH). We used an interspecific hybrid Silene latifolia ×Silene viscosa to compare two different genomes simultaneously on one slide. By using GISH with genomic DNA from another closely related species as a probe, we directly compared the level of relatedness between the genomes of the studied species and parental species. This experimental design enabled us to approximately estimate evolutionary relationships between the genome of tested plant species and genomes of both parental species of the hybrid by using the ratio of intensities of fluorescence signals. We tested this technique in various Silene species and the results were in accordance with the topology of the phylogenetic tree we constructed based on rDNA sequences. The results were also well correlated with phylogenetic distances between species that we estimated from an rDNA-based phylogenetic tree. Our experimental approach could help to improve tree topology and serve as a useful complementary tool in molecular phylogenetic studies in related species.
Similar content being viewed by others
References
Buzek J, Koutnikova H, Houben A et al. (1997) Isolation and characterization of X chromosome-derived DNA sequences from a dioecious plant Melandrium album. Chromosome Res 5: 57–5.
Chase MW, Knapp S, Cox AV et al. (2003) Molecular systematics, GISH and the origin of hybrid taxa in Nicotiana (Solanaceae). Ann Bot 92: 107–27.
Cheng BF, Séguin-Swartz G, Somers DJ (2002) Cytogenetic and molecular characterization of intergeneric hybrids between Brassica napus and Orychophragmus violaceus. Genome 45: 110–15.
Desel C, Jansen R, Dedong G, Schmidt T (2002) Painting of parental chromatin in Beta hybrids by multi-color fluorescent in situ hybridization. Ann Bot 89: 171–81.
Desfeux C, Maurice S, Henry JP, Lejeune B, Gouyon PH (1996) Evolution of reproductive systems in the genus Silene. Proc R Soc Lond Ser B Biol Sci 263: 409–14.
Galtier N, Gouy M, Gautier C (1996) SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12: 543–48.
Gonzalez G, Comas C, Confalonieri V, Naranjo CA, Poggio L (2006) Genomic affinities between maize and Zea perennis using classical and molecular cytogenetic methods (GISH-FISH). Chromosome Res 14: 629–35.
Heslop-Harrison JS, Harrison GE, Leitch IJ (1992) Reprobing of DNA/DNA in situ hybridisation preparations. Trends Genet 8: 372–73.
Hobza R, Vyskot B (2007) Laser microdissection-based analysis of plant sex chromosomes. Methods Cell Biol 82: 433–53.
Hobza R, Lengerova M, Svoboda J, Kubekova H, Kejnovsky E, Vyskot B (2006) An accumulation of tandem DNA repeats on the Y chromosome in Silene latifolia during early stages of sex chromosome evolution. Chromosoma 115: 376–82.
Ji Y, Pertuzé R, Chetelar RT (2004) Genome differentiation by GISH in interspecific and intergeneric hybrids of tomato and related nightshade. Chromosome Res 12: 107–16.
Jiang J, Gill BS (2006) Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research. Genome 49: 1057–068.
Jurgens A, Witt T, Gottsberger G (2002) Pollen grain numbers, ovule numbers and pollen-ovule ratios in Caryophylloideae: correlation with breeding systems, pollination, life form, style number, and sexual system. Sex Plant Reprod 14: 279–89.
Kato A, Vega JM, Han F, Lamb JC, Birchler JA (2005) Advances in plant chromosome identification and cytogenetic techniques. Curr Opinion Plant Biol 8: 148–54.
Kiss T, Kiss M, Solymosy F (1989) Nucleotide sequence of a 25S rRNA gene from tomato. Nucleic Acids Res 17: 796.
Kopecky D, Loureiro J, Zwierzykowski Z, Ghesquiere M, Dolezel J (2006) Genome constitution and evolution in Lolium ×Festuca hybrid cultivars (Festulolium). Theor Appl Genet 113: 731–42.
Lengerova M, Kejnovsky E, Hobza R, Macas J, Grant SR, Vyskot B (2004) Multicolor FISH mapping of the dioecious model plant, Silene latifolia. Theor Appl Genet 108: 1193–199.
Lim KY, Matyasek R, Lichtenstein CP, Leitch AR (2000) Molecular cytogenetic analyses and phylogenetic studies in the Nicotiana section Tomentosae. Chromosoma 109: 245–58.
Markova M, Lengerova M, Zluvova J, Janousek B, Vyskot B (2006) Karyological analysis of an interspecific hybrid between the dioecious Silene latifolia and the hermaphroditic Silene viscosa. Genome 49: 373–79.
Milner M, Patwardhan V, Bansode A et al. (2003) Constructing 3D phylogenetic trees. Curr Sci 85: 1471–487.
Neumann P, Koblizkova A, Navratilova A, Macas J (2006) Significant expansion of Vicia pannonica genome size mediated by amplification of a single type of giant retroelement. Genetics 173: 1047–056.
Nicolas M, Marais G, Hykelova V et al. (2005) A gradual process of recombination restriction in the evolutionary history of the sex chromosomes in dioecious plants. PLoS Biol 3: e4.
Oxelman B, Liden M (1995) Generic boundaries in the tribe Sileneae (Caryophyllaceae) as inferred from nuclear rDNA sequences. Taxon 44: 525–42.
Popp M, Oxelman B (2004) Evolution of a RNA polymerase gene family in Silene (Caryophyllaceae) — incomplete concerted evolution and topological congruence among paralogues. Syst Biol 53: 914–32.
Sharma S, Raina SN (2005) Organization and evolution of highly repeated satellite DNA sequences in plant chromosomes. Cytogenet Genome Res 109: 15–6.
Schwarzacher T, Heslop-Harrison P (2000) Practical in situ Hybridization. New York: Springer-Verlag.
Soltis PS, Soltis DA, Wolf PG, Nickrent DE, Chaw SM, Chapman RL (1999) The phylogeny of land plants inferred from 18S rDNA sequences: pushing the limits of rDNA signal? Mol Biol Evol 16: 1774–784.
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–680.
Vyskot B, Hobza R (2004) Gender in plants: sex chromosomes are emerging from the fog. Trends Genet 20: 432–38.
Whelan S, Lio P, Goldman N (2001) Molecular phylogenetics: state-of-the art methods for looking into the past. Trends Genet 17: 262–72
Zluvova J, Lengerova M, Markova M et al. (2005) The inter-specific hybrid Silene latifolia ×S. viscosa reveals early events of sex chromosome evolution. Evol Dev 7: 327–36.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Markova, M., Michu, E., Vyskot, B. et al. An interspecific hybrid as a tool to study phylogenetic relationships in plants using the GISH technique. Chromosome Res 15, 1051–1059 (2007). https://doi.org/10.1007/s10577-007-1180-8
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10577-007-1180-8