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QTL mapping of sheath blight resistance in a deep-water rice cultivar

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Abstract

Sheath blight, caused by the pathogen Rhizoctonia solani Kühn, is one of the most serious diseases of rice and leads to severe yield loss worldwide. A recombinant inbred line (RIL) population consisting of 121 lines was constructed from a cross between HH1B and RSB03, the latter of which is a deep-water rice variety. Five traits were used to evaluate sheath blight resistance, namely disease rating (DR), lesion length (LL), lesion height (LH), relative lesion length [RLL, the ratio of LL to plant height (PH)], and relative LH (RLH, the ratio of LH to PH). Using the RIL population and 123 molecular markers, we identified 28 quantitative trait loci (QTLs) for the five traits in two environments. These QTLs are located on nine chromosomes and most of them are environment specific. A major QTL for DR (qSBR1) on chromosome 1 was identified with contributions of 12.7% at Shanghai and 42.6% at Hainan, and it collocated with a QTL for PH. The allele at this locus from RSB03 enhances sheath blight resistance and increases PH. Another QTL for DR on chromosome 7 was adjacent to QTLs for heading date (HD) and four other disease traits. RSB03 also carries the resistant allele at this locus and shortens HD. The susceptible parent, HH1B, provides the resistance allele at the locus qSBR8, where QTLs for four other disease traits were identified. QTL mapping results showed that most QTLs for LL, LH, RLL, and RLH are collocated with QTLs for DR. Three QTLs for DR are independent from HD, PH, and four other disease traits, while four QTLs are closely related to HD and PH. Four QTLs for LL, LH, RLL, and RLH are independent from DR, HD, and PH, while there is only one region harboring QTLs for these four traits and HD. Correlation analysis and QTL mapping results indicated that LL, LH, RLL, and RLH might be important indices, like DR, for evaluating the level of resistance to rice sheath blight.

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Acknowledgments

We are grateful to the two anonymous reviewers for their critical reading of the manuscript. This work was supported by grants from the National Program on the Development of Basic Research in China (2010CB125901), Chinese Ministry of Agriculture (National Special Program for Transgenic Research, 2008ZX08001-002), and the Shanghai Science and Technology Development Funds (09ZR1428200).

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Authors and Affiliations

  1. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Dong Fu & Lijun Luo

  2. Shanghai Agrobiological Gene Center, Shanghai, 201106, China

    Dong Fu, Liang Chen, Guohui Yu, Yi Liu, Qiaojun Lou, Hanwei Mei, Liang Xiong, Mingshou Li, Xiaoyan Xu & Lijun Luo

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  1. Dong Fu
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  2. Liang Chen
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  3. Guohui Yu
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  4. Yi Liu
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  5. Qiaojun Lou
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  6. Hanwei Mei
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  7. Liang Xiong
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  8. Mingshou Li
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  9. Xiaoyan Xu
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  10. Lijun Luo
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Corresponding author

Correspondence to Lijun Luo.

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Dong Fu and Liang Chen contributed equally to this work.

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Table S1

Performance of traits related to resistance to sheath blight in some rice cultivars (DOC 41 kb)

Table S2

Marker distributions in the linkage map of recombinant inbred lines (RILs) derived from the cross of HH1B and RSB03 (DOC 118 kb)

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Fu, D., Chen, L., Yu, G. et al. QTL mapping of sheath blight resistance in a deep-water rice cultivar. Euphytica 180, 209–218 (2011). https://doi.org/10.1007/s10681-011-0366-5

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  • DOI: https://doi.org/10.1007/s10681-011-0366-5

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