Abstract
Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most important diseases of common wheat (Triticum aestivum L.). China has the largest stripe rust epidemic areas in the world and yield losses can be large. Aegilops tauschii Coss, the D-genome progenitor of common wheat, includes two subspecies, tauschii and strangulata (Eig) Tzvel. The ssp. strangulata accession AS2388 is highly resistant to the prevailing physiological races of PST in China, and possesses a single dominant gene for stripe rust resistance. In order to tag this gene, AS2388 was crossed with the highly susceptible ssp. tauschii accession AS87. The parents, F2 plants, and F2:3 families were tested at adult plant stage in field trials with six currently prevailing races. Simple sequence repeat (SSR) primers were used to identify molecular markers linked to the resistance gene. SSR markers Xwmc285 and Xwmc617 were linked to the resistance gene on chromosome arm 4DS flanking it at 1.7 and 34.6 cM, respectively. Based on the chromosomal location, this gene temporarily designated as YrAS2388 is probably novel. The resistance in Ae. tauschii AS2388 was partially expressed in two newly developed synthetic hexaploid backgrounds.
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References
Chen XM, Jones SS, Line RF (1995) Chromosomal location of genes for stripe rust resistance in spring wheat cultivars Compair, Fielder, Lee and Lemhi and interactions of aneuploid wheats with races of Puccinia striiformis. Phytopathology 85:375–381
Chen QJ, Zhang LQ, Yuan ZW, Yan ZH, Zheng YL, Sun GL, Liu DC (2008) Empirical verification of heterogeneous DNA fragments generated from wheat genome-specific SSR primers. Can J Plant Sci 88:1065–1071
Chen WQ, Wu LR, Liu TG, Xu SC (2009) Race dynamics diversity, and virulence evolution in Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust in China from 2003 to 2007. Plant Dis 93:1093–1101
Cheng P, Chen XM (2010) Molecular mapping of a gene for stripe rust resistance in spring wheat cultivar IDO377s. Theor Appl Genet 121:195–204
He MZ, Wang LM, Zhang ZY, Xu SC, Wang LL, Xin ZY (2007) Identification and molecular mapping of a novel stripe rust resistance gene in a Triticum durum-Aegilops tauschii amphiploid CI108. Acta Agron Sin 33:1045–1050
Herrera-Foessel SA, Lagudah ES, Huerta-Epino J, Hayden MJ, Bariana HS, Singh D, Singh RP (2010) New slow-rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked. Theor Appl Genet. doi:10.1007/s00122-010-1439-x
Jakaska V (1995) Isoenzymes in the evaluation of germplasm diversity in wild diploid relatives of cultivated wheat. In: Damania AB (ed) Biodiversity and wheat improvement. ICARDA, Wiley-Sayce Publ, London, pp 247–257
Kang Z, Zhao J, Han D, Zhang H, Wang X, Wang C, Han Q, Guo J, Huang L (2010) Status of wheat rust research and control in China. BGRI 2010 Technical Workshop, 30–31 May 2010, St Petersburg, Russia, pp 1–21
Kema GHJ, Lange W, Van Silphout CH (1995) Differential suppression of stripe rust resistance in synthetic wheat hexaploids derived from Triticum turgidum subsp. dicoccoides and Aegilops squarrosa. Phytopathology 85:425–429
Kihara H, Yamashita K, Tanaka M (1965) Morphological, physiological, genetic and cytological studies in Aegilops and Triticum collected from Pakistan, Afghanistan and Iran. In: Yamashita K (ed) Results of the Kyoto University scientific expedition to the Karakoram and Hindukush. Kyoyo University, Kyoto, Japan, pp 1–118
Knaggs P, Ambrose MJ, Reader SM, Miller TE (2000) Morphological characterization and evaluation of the subdivision of Aegilops tauschii Coss. Wheat Infor Serv 91:15–19
Li Q, Chen XM, Wang MN, Jing JX (2010) Yr45, a new wheat gene for stripe rust resistance on the long arm of chromosome 3D. Theor Appl Genet. doi:10.1007/s00122-010-1435-1
Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with Mapmaker/EXP3.0. Whitehead Institute Techn Rep, 3rd edn. Whitehead Institute, Cambridge, USA
Liu RH, Meng JL (2003) MapDraw: a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Hereditas (Beijing) 25:317–321
Liu DC, Zhang LQ, Yan ZH, Lan XJ, Zheng YL (2010) Stripe rust resistance in Aegilops tauschii and its genetic analysis. Genet Resour Grop Evol 57:325–328
Ma H, Singh RP, Mujeeb-Kazi A (1995) Resistance to stripe rust in Triticum turgidum, T. tauschii and their synthetic hexaploids. Euphytica 82:117–124
Marais F, Marais A, McCallum B, Pretorius Z (2009) Transfer of leaf rust and stripe rust resistance genes Lr62 and Yr42 from Aegilops neglecta Req. ex Bertol. to common wheat. Crop Sci 49:871–879
McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers DJ, Appels R, Devos KM (2008) Catalogue of gene symbols for wheat. In: Appels R, Eastwood R, Lagudah E, Langridge P, Mackay M, McIntyre L, Sharp P (eds) Proc 11th Int Wheat Genet Symp. Sydney University Press, Sydney, pp 143–150
McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2010) Catalogue of gene symbols for wheat: 2010 supplement. Annual Wheat Newsletter 56:273–282
McNeal FH, Koebner CF, Smith EP, Tate WS, Russell TS (1971) A uniform system for recording and processing cereal research data. USDA-ARS Bull 42:34–121
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Rizwan S, Ahmad I, Ashraf M, Sahi GM, Mirza JI, Ratto A-U-R, Mujeeb-Kazi A (2007) New sources of wheat yellow rust (Puccinia striiformis f. sp. tritici) seedling resistance. Pak J Bot 39:595–602
Röder MS, Korzun V, Wendenhake K, Plaschke J (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Rogers SO, Bendich AJ (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5:69–76
Singh RP, Nelson JC, Sorrells ME (2000) Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Sci 40:1148–1155
Somers DJ, Isaac P, Edwards K (2004) A high density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114
Tixier MH, Sourdille P, Röder MS, Leroy P, Bernard M (1997) Detection of wheat microsatellites using a non radioactive silver-nitrate staining method. J Genet Breed 51:175–177
Wan AM, Zhao ZH, Chen XM, He ZH, Jin SL, Jia QZ, Yao G, Yang JX, Wang BT, Li GB, Bi YQ, Yuan ZY (2004) Wheat stripe rust epidemics and virulence of Puccinia striiformis f. sp. tritici in china in 2002. Plant Dis 88:896–904
Wan AM, Chen XM, He ZH (2007) Wheat stripe rust in China. Aust J Agric Res 58:605–619
Wang FL, Wu LR, Wan AM (1995) Studies on virulence variation of wheat stripe rust population in China. Acta Agron Sin 28:8–14
Yang WY, Yu Y, Zhang Y, Hu XR, Wang Y, Zhou YC, Lu BR (2003) Inheritance and expression of stripe rust resistance in common wheat (Triticum aestivum) transferred from Aegilops tauschii and its utilization. Hereditas 139:49–55
Yen C, Yang JL, Liu XD (1983) The distribution of Aegilops tauschii Cosson in China and with reference to the origin of the Chinese common wheat. In: Sankamoto S (ed) Proc 6th Int Wheat Gene Symp. Kyoto University, Japan, pp 55–58
Yildirim A, Jones SS, Murray TD, Cox TS, Line RF (1995) Resistance to stripe rust and eyespot diseases of wheat in Triticum tauschii. Plant Dis 79:1230–1236
Zhang LQ, Liu DC, Zheng YL, Yan ZH, Dai SF, Li YF, Jiang Q, Ye YQ, Yen Y (2010) Frequent occurrence of unreduced gametes in Triticum turgidum-Aegilops tauschii hybrids. Euphytica 172:285–294
Acknowledgments
This study was supported by National Natural Science Foundation of China (31071418), the 973 Program (2009CB118300), the 100-Talent Program of CAS, and by the Science and Technology Commission of Sichuan Province. We thank Professor Robert A McIntosh, at University of Sydney, for reviewing this manuscript.
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Huang, L., Zhang, LQ., Liu, BL. et al. Molecular tagging of a stripe rust resistance gene in Aegilops tauschii . Euphytica 179, 313–318 (2011). https://doi.org/10.1007/s10681-010-0330-9
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DOI: https://doi.org/10.1007/s10681-010-0330-9