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Transgenic soybean with low phytate content constructed by Agrobacterium transformation and pollen-tube pathway

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Abstract

The expression of a microbial phytase in transgenic plants may create a new biochemical pathway that mobilizes its endogenous phytate and release inorganic phosphate from it, so that more phosphorus is available for plant growth. In this study, transgenic soybean plants were generated via both Agrobacterium transformation and pollen tube pathway with the PhyA gene of Aspergillus ficuum. The optimal concentrations of plant hormones including N6-benzylaminopurine (BAP), gibberellin (GA3) and indole-3-butyric acid (IBA) were tested based on their effectiveness on promoting the growth of transgenic explants. Genomic PCR results and Southern blot hybridization analysis showed that transgenic soybean plants selected for resistance to kanamycin contained the phyA transgene. The transgenic soybean plants with phyA gene integrated in their genome exhibited lower amount of phytate in different soybean tissues including leaf, stem and root, which indicated that engineering crop plants with a higher expression level of heterologous phytase could improve the degradation of phytate and potentially in turn mobilize more inorganic phosphate from phytate and thus reduce phosphate load on agricultural ecosystems.

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Acknowledgments

The Project Supported by Natural Science Foundation of Hebei Province (C2009000868) and Project of Transgenic Crops of Ministry Science and Technology Development of China (2009ZX08004-004B).

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Authors and Affiliations

  1. School of Agriculture and Bioengineering, Tianjin University, Tianjin, 300072, People’s Republic of China

    Shaohui Yang & Jiehua Wang

  2. College of Life Sciences, Nankai University, Tianjin, 300071, People’s Republic of China

    Minggang Li

  3. Hebei Normal University of Science & Technology, Changli, 066600, People’s Republic of China

    Guilan Li

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  1. Shaohui Yang
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  2. Guilan Li
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  3. Minggang Li
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  4. Jiehua Wang
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Correspondence to Jiehua Wang.

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Yang, S., Li, G., Li, M. et al. Transgenic soybean with low phytate content constructed by Agrobacterium transformation and pollen-tube pathway. Euphytica 177, 375–382 (2011). https://doi.org/10.1007/s10681-010-0262-4

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  • DOI: https://doi.org/10.1007/s10681-010-0262-4

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