Abstract
Use of biotechnology techniques combined with mutagenesis in Musa spp. allows genetic variability to be increased to improve important agronomic characters. Damage caused to Musa plantations by strong winds and tropical hurricanes makes it necessary to select clones with reduced height and better resistance to wind. The objective of this study is to develop mutant clones of FHIA-21 (Musa AAAB) with reduced height, a good agronomical trait. Multiple buds obtained by in vitro culture were subjected to gamma radiation from 60Co sources at dose of 25 Gy. Irradiated buds were multiplied, elongated, rooted, and then planted (10,000 plants) in the field. In the clonal study, 98 mutant clones with lower pseudostem height compared with the original cultivar were selected. Four mutants with reduced height at bunch emergency were selected (IBP 14-23, IBP 17-13, IBP 24-14, and IBP 47-4). Mutants IBP 24-14 and IBP 47-4 showed poor agronomical features, reduced foliar area, and resulted of no commercial value. The results indicated that mutant IBP 14-23 retains the character of resistance to black sigatoka from its parent FHIA-21, combined with important agronomical traits during the third culture cycle, making it a promising cultivar material, representing 0.01% selection efficiency. This is the first report of improvement for height reduction of the tetraploid hybrid FHIA-21 through physical mutagenesis.
Similar content being viewed by others
Abbreviations
- FHIA:
-
Fundación Hondureña de Investigación Agrícola
- MS:
-
Murashige and Skoog (1962) medium
- 6-BAP:
-
6-Benzylaminopurine
- IAA:
-
Indolacetic acid
- IBA:
-
Indole-3-butyric acid
- NTL:
-
Number of total leaves
- NFL:
-
Number of functional leaves
References
Álvarez J (1997) Introducción, evaluación, multiplicación y diseminación de los híbridos FHIA en Cuba. INFOMUSA 6(2):10–14
Basu SK, Acharya SN, Thomas JE (2008) Genetic improvement of fenugreek (Trigonella foenum-graecum L.) through EMS induced mutation breeding for higher seed yield under western Canada prairie conditions. Euphytica 160(2):249–258
Bermúdez I, Orellana P, Pérez Ponce J, Clavero J, Veitía N, Romero C, Mujica R, Garcia L (2000) Improvement of the hybrid plantain clone FHIA-21 by in vitro mutagenesis. INFOMUSA 9(1):16–19
Chai M, Ho YW, Liew KW, Asif JM (2004) Biotechnology and in vitro mutagenesis for banana improvement. In: Jain SM, Swennen R (eds) Banana improvement: cellular, molecular biology, and induced mutations. Science, Enfield, USA, pp 59–77
De Guzman EV, Del Rosario AG, Pagcaliwagan PC (1982) Production of mutants by irradiation of in vitro cultured tissues of coconut and banana and their mass propagation by tissue culture technique. In: Induced mutations in vegetative propagated plants 11, Vienna, IAEA, pp 113–118
FAOSTAT. Food and Agriculture organization of the United Nations. FAO (2009) http://faostat.fao.org/site. Accessed 16 October 2009
FHIA (1997) Plantain plant FHIA 21. Patent number 9 791. US Patent
FHIA (2002) http://www.fhia.hn. Accessed 14 January 2002
Ganapathi TR, Meenakshi S, Suprasanna P, Ujjappa KM, Bapat VA, D’Souza SF (2008) Field performance and RAPD analysis of gamma-irradiated variants of banana cultivar ‘Giant Cavendish’ (AAA). Int J Fruit Sci 8(3):147–159
Hernández AJ, Pérez JM, Bosch DI, Riveros LR, Camacho ED, Ruiz JC, Jaimez ES, Mersán RB, Obregón AS (1999) Nueva versión de clasificación genética de los suelos de Cuba. In: En Baecaz LL (ed), AGRINFOR, ISBN: 959-246-022-1, Habana, pp 64
Hernández R, Rodríguez R, Ramírez T, Cañal MJ, Guillen D, Noceda C, Escalona M, Corujo M, Ventura J (2007) Genetic and morphoagronomic characterization of plantain variants of Musa AAB clone ‘CEMSA ¾’. J Food Agric Environ 5(1):220–223
Ho YW, Mak C, Tan P (1994) Strategies in the improvement of banana cultivars for commercial scale cultivation. In: Proceedings of international planters conference, Kuala Lumpur, Malaysia, pp 71–82
Huang X, Lu XY, Zhao JT, Chen JK, Dai XM, Xiao W, Chen YP, Chen YF, Huang XL (2009) MaSERK1 Gene expression associated with somatic embryogenic competence and disease resistance response in banana (Musa spp.). Plant Mol Biol. doi:10.1007/s111050090150
INIBAP (1996) Banana (Musa spp.) Descriptors for banana (Musa spp.). International Plant Genetic Resources Institute, Rome, Italy. International Network for the Improvement of Banana and Plantain, Montpellier, France; and Centre de coopération internationale en recherche agronomique pour le développement, Montpellier, France, p 55
Kulkarni VM, Ganapathi TR, Suprasanna P, Bapat VA (2007) In vitro mutagenesis in banana (Musa spp.) using gamma irradiation. In: Jain SM, Haggma H (eds) Protocols for micropropagation of woody trees and fruits. Springer, New York, pp 543–549
López J, Strosse H, Ventura J, Sanchéz R, Rodriguez S, Swennen R, Panis B, Afza R (2004) Field evaluation of potential mutants obtained after gamma irradiation of banana and plantain (Musa spp.) shoot-tip and embryogenic cell cultures. In: Jain SM, Swennen R (eds) Banana improvement: cellular, molecular biology, and induced mutations. Science, Enfield, USA, pp 59–77
MINAGRI (1991) Instructivo Técnico del Plátano. La Habana, Cuba, p 124
Mishra PJ, Ganapathi TR, Suprasanna P, Bapat VA (2007) Effect of single and recurrent gamma irradiation on in vitro shoot cultures of banana. Int J Fruit Sci 7(1):47–57
Murashige T, Skoog R (1962) A revised medium for rapid growth and bio-assay with tobacco tissue culture. Physiol Plant 15:473–497
Novak FJ, Donini B, Hermelin T, Micke A (1987) Potential for banana and plantain improvement through in vitro mutation breeding, ACORBAT, Proceedings VIII workshop, Turrialba, Costa Rica, CATIE, pp 67–70
Novak FJ, Afza R, Van Duren M, Omar MS (1990) Mutation induction by gamma irradiation of in vitro cultured shoot tips of banana and plantain (Musa cvs.). Trop Agric 67:21–28
Orellana PP (1995) Tecnología para la micropropagación in Vitro de clones de Musa spp. Tesis para aspirar por el grado científico de Doctor en Ciencias Agrícolas. UCLV. IBP. Santa Clara. 120 p
Orellana PP, Pérez J, Agramonte D, Gómez R, Jimenez E, Martinez S, Almaguer E, Gómez R (1991) The plantain micropropagation to commercial level in Cuba. ACEVIC. Cientific Bull 3(3):29–38
Ortiz R, Vuylsteke D (1995) Inheritance of dwarfism in plantain (Musa spp., AAB group.). Plant Breed 114:446–468
Roux NS (2004) Mutation induction in Musa-review. In: Jain SM, Swennen R (eds) Banana improvement: cellular, molecular biology, and induced mutations. Science, Enfield, USA, pp 23–32
Sandoval J, Kerbellec F, Cote F, Doumas P (1995) Distribution of endogenous gibberellins in dwarf and giant off’types banana (Musa AAA, cv. Grand nain) plants from in vitro propagation. Plant Growth Regul 17:219–224
Smith MK, Hamill SD, Langdon PW, Giles JE, Doogan VJ, Pegg KG (2006) Towards the development of a Cavendish banana resistant to race 4 of fusarium wilt: gamma irradiation of micropropagated Dwarf Parfitt (Musa spp., AAA group, Cavendish subgroup). Aust J Exp Agric 46:107–113
Vuylsteke D, Swennen R, De Langhe E (1991) Somaclonal variation in plantains (Musa spp., AAB group) derived from shoot-tip culture. Fruits 46:429–439
Acknowledgments
The authors thank Dion Desmon Daniels PhD for help with the technical revision in preparing the manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bermúdez-Caraballoso, I., García, L.R., Veitía, N. et al. Mutant plantains (Musa spp.) with height reduction obtained by in vitro mutagenesis. Euphytica 176, 105–112 (2010). https://doi.org/10.1007/s10681-010-0233-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10681-010-0233-9