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Associations of PCR markers with freezing tolerance and photosynthetic acclimation to cold in winter barley

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Abstract

Associations of PCR markers with freezing tolerance and acclimation of photosynthetic apparatus to cold were tested on 28 winter barley cultivars and advanced breeding forms to select alleles for practical application in marker assisted selection (MAS). We found significant associations between freezing tolerance evaluated with field-laboratory method (FLM) and markers located on 5H chromosome in region of gene Fr-H2 (bin9-10: Xbmag812, Xmwg2230) and region of gene Fr-H1 (bin11: Xmwg514, HvBM5, Xmwg644). Additionally, significant associations with photochemical quenching of chlorophyll a fluorescence (qP) were found for PCR markers Xpsr115 and Xmwg2062. In our study variation in the promoter region of Vrn-H1 (HvBM5) was directly connected with freezing tolerance of plants partially de-acclimated in the field. The results obtained here showed that different loci of freezing tolerance may play role in variable selection pressure of winter conditions.

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Acknowledgments

The studies were funded by Polish Ministry of Science and Higher Education Grant 2 P06A 002 28.

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Authors and Affiliations

  1. Department of Plant Physiology, University of Agriculture in Krakow, Podłużna 3, 30-239, Kraków, Poland

    M. Rapacz

  2. Department of Biochemistry and Biotechnology, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy Av. 6, 35-959, Rzeszów, Poland

    M. Tyrka

  3. Department of Quality Evaluation and Cereal Breeding Methods, Institute of Plant Breeding and Acclimatization in Krakow, Zawiła 4, 30-423, Kraków, Poland

    M. Gut

  4. Szelejewo Plant Breeding, 63-820, Piaski, Poland

    W. Mikulski

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  1. M. Rapacz
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  2. M. Tyrka
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  4. W. Mikulski
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Correspondence to M. Rapacz.

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Rapacz, M., Tyrka, M., Gut, M. et al. Associations of PCR markers with freezing tolerance and photosynthetic acclimation to cold in winter barley. Euphytica 175, 293–301 (2010). https://doi.org/10.1007/s10681-010-0127-x

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  • DOI: https://doi.org/10.1007/s10681-010-0127-x

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