Skip to main content
SpringerLink
Log in

Construction of a linkage map and identification of DNA markers linked to Fom-1, a gene conferring resistance to Fusarium oxysporum f.sp. melonis race 2 in melon

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Resistance to Fusarium oxysporum f.sp. melonis race 2 is conferred by a single dominant gene, Fom-1 in melon. Here, we identified DNA markers tightly linked to Fom-1 that could be used for marker assisted selection in breeding programs. First, we developed 125 F2 plants derived from the cross between melon lines P11 (fom-1fom-1) and MR-1 (Fom-1Fom-1). Using the F2 population, we constructed a linkage map including 14 SSR markers which had not been mapped previously. Fom-1 was confirmed to be allocated to linkage group 7. Then, we identified four AFLP markers using bulked segregant analysis. The AFLP marker TAG/GCC-470 was completely linked to Fom-1 and other three markers were mapped near Fom-1. TAG/GCC-470 and TCG/GGT-400 were respectively converted to STS and CAPS markers. Usefulness of DNA markers was confirmed in the analysis with several melon cultivars and lines.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Brotman Y, Kovalski I, Dogimont C, Pitrat M, Portnoy V, Katzir N, Perl-Treves R (2005) Molecular markers linked to papaya ring spot virus resistance and Fusarium race 2 resistance in melon. Theor Appl Genet 110:337–345. doi:10.1007/s00122-004-1845-z

    Article  PubMed  CAS  Google Scholar 

  • Chiba N, Suwabe K, Nunome T, Hirai M (2003) Development of microsatellite markers in melon (Cucumis melo L.) and their application to major cucurbit crops. Breed Sci 53:21–27. doi:10.1270/jsbbs.53.21

    Article  CAS  Google Scholar 

  • Danin-Poleg Y, Burger Y, Schreiber S, Katzir N, Cohen R (1999) Identification of the gene for resistance to Fusarium wilt races 0 and 2 in Cucumis melo ‘Dulce’. Cucurb Genet Coop Rep 22:19–20

    Google Scholar 

  • Danin-Poleg Y, Reis N, Tzuri G, Katzir N (2001) Development and characterization of microsatellite markers in Cucumis. Theor Appl Genet 102:61–72. doi:10.1007/s001220051618

    Article  CAS  Google Scholar 

  • Gonzalo MJ, Oliver M, Garcia-Mas J, Monfort A, Dolcet-Sanjuan R, Katzir N, Arús P, Monforte AJ (2005) Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.). Theor Appl Genet 110:802–811. doi:10.1007/s00122–004-1814-6

    Article  PubMed  CAS  Google Scholar 

  • Herman R, Perl-Treves R (2007) Characterization and inheritance of a new source of resistance to Fusarium oxysporum f. sp. melonis race 1.2 in Cucumis melo. Plant Dis 91:1180–1186. doi:10.1094/PDIS-91-9-1180

    Article  Google Scholar 

  • Joobeur T, King JJ, Nolin SJ, Thomas CE, Dean RA (2004) The fusarium wilt resistance locus Fom-2 of melon contains a single resistance gene with complex features. Plant J 39:283–297. doi:10.1111/j.1365-313X.2004.02134.x

    Article  PubMed  CAS  Google Scholar 

  • Katzir N, Danin-Poleg Y, Tzuri G, Karchi Z, Lavi U, Cregan PB (1996) Length polymorphism and homologies of microsatellites in several Cucurbitaceae species. Theor Appl Genet 93:1282–1290. doi:10.1007/BF00223461

    Article  CAS  Google Scholar 

  • Kumar LS (1999) DNA markers in plant improvement: an overview. Biotechnol Adv 17:143–182. doi:10.1016/S0734-9750(98)00018-4

    Article  PubMed  CAS  Google Scholar 

  • Kurt S, Baran B, Sari N, Yetisir H (2002) Physiologic races of Fusarium oxysporum f.sp. melonis in the southeastern Anatolia Region of Turkey and varietal reactions to races of the pathogen. Phytoparasitica 30:395–402. doi:10.1007/BF02979687

    Article  Google Scholar 

  • Liu Y-G, Mitsukawa N, Oosumi T, Whittier RF (1995) Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J 8:457–463. doi:10.1046/j.1365-313X.1995.08030457.x

    Article  PubMed  CAS  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832. doi:10.1073/pnas.88.21.9828

    Article  PubMed  CAS  Google Scholar 

  • Morales M, Luís-Arteaga M, Álvarez JM, Dolcet-Sanjuan R, Monfort A, Arús P, Garcia-Mas J (2002) Marker saturation of the region flanking the gene NSV conferring resistance to the melon necrotic spot Carmovirus (MNSV) in melon. J Am Soc Hortic Sci 127:540–544

    CAS  Google Scholar 

  • Moretti A, Belisario A, Tafuri A, Ritieni A, Corazza L, Logrieco A (2002) Production of beauvericin by different races of Fusarium oxysporum f. sp. melonis, the Fusarium wilt agent of muskmelon. Eur J Plant Pathol 108:661–666. doi:10.1023/A:1020674929084

    Article  CAS  Google Scholar 

  • Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325. doi:10.1093/nar/8.19.4321

    Article  PubMed  CAS  Google Scholar 

  • Nakazumi H, Hirai G (2004) Diallel analysis for resistance of melon (Cucumis melo) to Fusarium wilt caused by Fusarium oxysporum f.sp. melonis race 1,2y. Breed Res 6:65–70. doi:10.1270/jsbbr.6.65

    Article  Google Scholar 

  • Namiki F, Shiomi T, Nishi K, Kayamura T, Tsuge T (1998) Pathogenic and genetic variation in the Japanese strains of Fusarium oxysporum f. sp. melonis. Phytopathology 88:804–810. doi:10.1094/PHYTO.1998.88.8.804

    Article  PubMed  CAS  Google Scholar 

  • Namiki F, Shimizu K, Satoh K, Hirabayashi T, Nishi K, Kayamura T, Tsuge T (2000) Occurrence of Fusarium oxysporum f. sp. melonis race 1 in Japan. J Gen Plant Pathol 66:12–17. doi:10.1007/PL00012915

    Article  Google Scholar 

  • Namiki F, Matsunaga M, Okuda M, Inoue I, Nishi K, Fujita Y, Tsuge T (2001) Mutation of an arginine biosynthesis gene causes reduced pathogenicity in Fusarium oxysporum f. sp. melonis. Mol Plant Microbe Interact 14:580–584. doi:10.1094/MPMI.2001.14.4.580

    Article  PubMed  CAS  Google Scholar 

  • Noguera FJ, Capel J, Alvarez JI, Lozano R (2005) Development and mapping of a codominant SCAR marker linked to the andromonoecious gene of melon. Theor Appl Genet 110:714–720. doi:10.1007/s00122-004-1897-0

    Article  PubMed  CAS  Google Scholar 

  • Oumouloud A, Arnedo-Andres MS, Gonzalez-Torres R, Alvarez JM (2008) Development of molecular markers linked to the Fom-1 locus for resistance to Fusarium race 2 in melon. Euphytica 164:347–356. doi:10.1007/s10681-008-9664-y

    Article  CAS  Google Scholar 

  • Perchepied L, Dogimont C, Pitrat M (2005) Strain-specific and recessive QTLs involved in the control of partial resistance to Fusarium oxysporum f. sp. melonis race 1.2 in a recombinant inbred line population of melon. Theor Appl Genet 111:65–74. doi:10.1007/s00122-005-1991-y

    Article  PubMed  CAS  Google Scholar 

  • Périn C, Hagen LS, De Conto V, Katzir N, Danin-Poleg Y, Portnoy V, Baudracco-Arnas S, Chadoeuf J, Dogimont C, Pitrat M (2002) A reference map of Cucumis melo based on two recombinant inbred line populations. Theor Appl Genet 104:1017–1034. doi:10.1007/s00122-002-0864-x

    Article  PubMed  CAS  Google Scholar 

  • Pitrat M, Hanelt P, Hammer K (2000) Some comments on infraspecific classification of cultivars of melon. Acta Hortic 510:29–36

    Google Scholar 

  • Risser G (1987) Controversy on resistance to Fusarium wilt in ‘Perlita’ (Cucumis melo L.). Cucurb Genet Coop Rep 10:60–61

    Google Scholar 

  • Risser G, Banihashemi Z, Davis DW (1976) A proposed nomenclature of Fusarium oxysporum f. sp. melonis races and resistance genes in Cucumis melo. Phytopathology 66:1105–1106

    Article  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. Humana Press, Totowa, pp 365–386

    Google Scholar 

  • Thomas CE (1986) Downy and powdery mildew resistant muskmelon breeding line MR-1. HortScience 21:329

    Google Scholar 

  • Touyama T, Kanbe M (2002) Development of PCR-based marker which linked to the Fom1 fusarium wilt resistance gene in melon (Cucumis melo L.). Res Bull Aichi Agric Res Ctr 34:49–53

    CAS  Google Scholar 

  • van Leeuwen H, Garcia-Mas J, Coca M, Puigdoménech P, Monfort A (2005) Analysis of the melon genome in regions encompassing TIR-NBS-LRR resistance genes. Mol Genet Genomics 273:240–251. doi:10.1007/s00438-004-1104-7

    Article  PubMed  CAS  Google Scholar 

  • van Ooijen JW (2006) JoinMap® 4, Software for the calculation of genetic linkage maps in experimental populations. Kyazma B.V., Wageningen

  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414. doi:10.1093/nar/23.21.4407

    Article  PubMed  CAS  Google Scholar 

  • Wang Y-H, Thomas CE, Dean RA (2000) Genetic mapping of a fusarium wilt resistance gene (Fom-2) in melon (Cucumis melo L.). Mol Breed 6:379–389. doi:10.1023/A:1009671925793

    Article  CAS  Google Scholar 

  • Zheng XY, Wolff DW (2000) Randomly amplified polymorphic DNA markers linked to Fusarium wilt resistance in diverse melons. HortScience 35:716–721

    CAS  Google Scholar 

  • Zheng XY, Wolff DW, Baudracco-Arnas S, Pitrat M (1999) Development and utility of cleaved amplified polymorphic sequences (CAPS) and restriction fragment length polymorphisms (RFLPs) linked to the Fom-2 fusarium wilt resistance gene in melon (Cucumis melo L.). Theor Appl Genet 99:453–463. doi:10.1007/s001220051257

    Article  CAS  Google Scholar 

  • Zink FW, Gubler WD (1985) Inheritance of resistance in muskmelon to Fusarium wilt. J Am Soc Hortic Sci 110:600–604

    Google Scholar 

  • Zink FW, Thomas CE (1990) Genetics of resistance to Fusarium oxysporum f. sp. melonis races 0, 1, and 2 in muskmelon line MR-1. Phytopathology 80:1230–1232. doi:10.1094/Phyto-80-1230

    Article  Google Scholar 

  • Zuniga TL, Zitter TA, Gordon TR, Schroeder DT, Okamoto D (1997) Characterization of pathogenic races of Fusarium oxysporum f. sp. melonis causing Fusarium wilt of melon in New York. Plant Dis 81:592–596. doi:10.1094/PDIS.1997.81.6.592

    Article  Google Scholar 

Download references

Author information

Author notes

  1. Takahiro Tezuka

    Present address: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, 599-8531, Japan

Authors and Affiliations

  1. Plant Biotechnology Institute, Ibaraki Agricultural Center, 3165-1 Ago, Kasama, Ibaraki, 319-0292, Japan

    Takahiro Tezuka, Keisuke Waki, Tomoko Ishikawa & Makoto Miyagi

  2. Chikusei Agricultural Extension Center, Ibaraki Agricultural Center, 615 Nikinari, Chikusei, Ibaraki, 308-0841, Japan

    Kazutoshi Yashiro & Maki Kuzuya

  3. Ibaraki Agricultural Academy, Ibaraki Agricultural Center, 4070-186, Nagaoka, Ibaraki, 311-3116, Japan

    Yasumasa Takatsu

Authors
  1. Takahiro Tezuka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Keisuke Waki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kazutoshi Yashiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maki Kuzuya
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tomoko Ishikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yasumasa Takatsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Makoto Miyagi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Takahiro Tezuka.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tezuka, T., Waki, K., Yashiro, K. et al. Construction of a linkage map and identification of DNA markers linked to Fom-1, a gene conferring resistance to Fusarium oxysporum f.sp. melonis race 2 in melon. Euphytica 168, 177–188 (2009). https://doi.org/10.1007/s10681-009-9881-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-009-9881-z

Keywords

Search

Navigation