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Three AFLP markers tightly linked to the genic male sterility ms 3 gene in chili pepper (Capsicum annuum L.) and conversion to a CAPS marker

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Abstract

Genic male sterility (GMS) has long been used as a tool for hybrid seed production in chili pepper (Capsicum annuum L.). We developed DNA markers linked to the GMS ms 3 gene in a segregating population using bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) techniques. The segregating population was subjected to BSA-AFLP with 512 primer combinations. Three AFLP markers (Eagg/Mccc276, Eagc/Mctt178, and Ecag/Mtgc204) were identified as tightly linked to the ms 3 locus. Among them, we converted the AFLP marker Ecag/Mtgc204 to the cleavage amplified polymorphic sequence (CAPS) marker, named GMS3-CAPS, based on sequencing analysis of internal and flanking regions for the markers between male-fertile and sterile plants. This marker will be useful for pepper breeding using the GMS system.

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Acknowledgments

This work was supported by a grant (number 20070101033138) from the Rural Development Administration, Republic of Korea. We are grateful to Yong-Jik Lee (Hana Seed Co., South Korea) for supplying the 292 seeds as material for marker development.

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Authors and Affiliations

  1. Research and Development Unit, Pepper and Breeding Institute, Business Incubator, College of Agriculture and Life Sciences, Seoul National University, Suwon, 441-853, South Korea

    Jundae Lee, Jae Bok Yoon, Jung-Heon Han, Won Phil Lee, Sang Hoon Kim & Hyo Guen Park

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  1. Jundae Lee
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  2. Jae Bok Yoon
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  3. Jung-Heon Han
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  4. Won Phil Lee
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  5. Sang Hoon Kim
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  6. Hyo Guen Park
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Correspondence to Jae Bok Yoon.

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Lee, J., Yoon, J.B., Han, JH. et al. Three AFLP markers tightly linked to the genic male sterility ms 3 gene in chili pepper (Capsicum annuum L.) and conversion to a CAPS marker. Euphytica 173, 55–61 (2010). https://doi.org/10.1007/s10681-009-0107-1

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  • DOI: https://doi.org/10.1007/s10681-009-0107-1

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