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In vitro induction and characterization of somaclonal variation for red rot and other agronomic traits in sugarcane

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Abstract

In the present investigation, optimal media compositions for enhancing the callus induction and subsequent plant regeneration were worked out in sugarcane (Saccharum spp. complex). The field grown somaclones were characterized for yield and quality contributing traits in comparison to donor variety CoJ 88. The MS + 2,4-D (4.0 mg/l) + kin (0.5 mg/l) + sucrose (30 g/l) was found to be the best for the callus induction among the nine media compositions used. The highest shoot regeneration was 34.65% on MS + BAP (0.5 mg/l) followed by 31.07% on MS + IAA (2.0 mg/l) + kin (0.5 mg/l) and the least 11.80% on MS + BAP (2.0 mg/l). The shoot elongation was optimum on MS + IBA (5.0 mg/l). The rooting was carried on MS + NAA (3.0 mg/l) + IBA (2.0 mg/l) + sucrose (70 g/l). The somaclones, on field evaluation, exhibited huge variability for characters viz., tiller number at 120 days of field transfer, number of millable canes, cane height, number of nodes and internodal length at time of harvest and also for HR Brix at 240 and 300 days after field transfer. In R0 generation, the variations induced for the characters such as stalk height after 120 days, HR Brix after 240 and 300 days of field transfer were negatively skewed with most of somaclones having values higher than the population mean values thereby offering adequate scope for improvement cane and quality. Elite somaclones marked for each trait were reported. Out of 228 somaclones inoculated with Cf 08 pathotype of red rot in R1, three were resistant, four were moderately resistant and 221 somaclones showed variable degree of susceptibility, whereas, to Cf 03 pathotype, fourteen were resistant, nineteen were moderately resistant and 195 showed variable degree of susceptibility. Three somaclones exhibited moderate resistance (MR) to both the pathotypes, whereas, donor variety CoJ 88 showed susceptible reaction. These somaclones will further enhance the germplasm for sugarcane breeding.

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Abbreviations

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

IAA:

Indole-3-aceticacid

IBA:

Indole-3-butyricacid

kin:

Kinetin (6-furfuryl aminopurine)

NAA:

Naphthaleneacetic acid

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Authors and Affiliations

  1. Department of Plant Breeding, Genetics and Biotechnology, Punjab Agricultural University, Ludhiana, Punjab, 141 004, India

    Gurpreet Singh, S. K. Sandhu, Madhu Meeta, Karnail Singh, Raman Gill & S. S. Gosal

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  1. Gurpreet Singh
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  2. S. K. Sandhu
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  3. Madhu Meeta
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  4. Karnail Singh
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  5. Raman Gill
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  6. S. S. Gosal
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Correspondence to Gurpreet Singh.

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Singh, G., Sandhu, S.K., Meeta, M. et al. In vitro induction and characterization of somaclonal variation for red rot and other agronomic traits in sugarcane. Euphytica 160, 35–47 (2008). https://doi.org/10.1007/s10681-007-9531-2

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  • DOI: https://doi.org/10.1007/s10681-007-9531-2

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