Skip to main content
SpringerLink
Log in

Effect of hardening on frost tolerance and fatty acid composition of leaves and stems of a set of faba bean (Vicia faba L.) genotypes

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Frost tolerance is a main component of winter-hardiness and improving it would promote faba bean (Vicia faba L.) cropping in cool-temperate regions. In many species, leaf fatty acid composition was found to be related to frost tolerance. The objective of this study was to determine, in a representative sample of genotypes, the effect of hardening on leaf and stem (1) frost tolerance and (2) fatty acid composition, and to seek correlations between them. First leaf, second leaf and stem of 31 faba bean genotypes were analyzed after hardening and without hardening. High frost tolerance of known winter genotypes and several experimental lines was shown. Hardening had a significant, positive effect on frost tolerance of all three organs. Stems were on average more frost tolerant than leaves. Hardening induced significant changes in the fatty acid composition: oleic acid decreased significantly in leaves by 3.24% and in stems by 1.77%, whereas linolenic acid increased in leaves by 6.28% and in stems by 9.06%. In stems, correlations between frost tolerance and fatty acid composition were not significant. Correlation coefficients strongly indicated that non-hardened oleic acid content, changes in oleic acid and in linoleic plus linolenic acid content in leaves partly explained their frost tolerance; 0.347 (P < 0.1) < |r| < 0.543 (P < 0.01). The results corroborate the importance of using genetic differences in the fatty acid metabolism in breeding grain legumes for frost tolerance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Arbaoui M (2007) Detailed genetic analysis of faba bean (Vicia faba L.) winter-hardiness and related traits, Ph.D. thesis, Georg-August University, Göettigen, Germany

  • Badaruddin M, Meyer DW (2001) Factors modifying frost tolerance of legume species. Crop Sci 41:1911–1916

    Article  Google Scholar 

  • Chandrasekar V, Sairam RK, Srivastava GC (2000) Physiological and biochemical responses of hexaploid and tetraploid wheat to drought stress. J Agron Crop Sci 185:219–227

    Article  CAS  Google Scholar 

  • Cyril J, Powell GL, Duncan RR, Baird WV (2002) Changes in membrane polar lipid fatty acids of seashore Paspalum in response to low temperature exposure. Crop Sci 42:2031–2037

    Article  CAS  Google Scholar 

  • Davidson NJ, Reid JB (1987) The influence of hardening and waterlogging on the frost resistance of subalpine eucalyptus. Aust J Bot 35:91–101

    Article  Google Scholar 

  • Davis DL, Gilbert WB (1970) Winter hardiness and changes in soluble protein fractions of bermudagrass. Crop Sci 10:7–9

    Article  CAS  Google Scholar 

  • Duc G, PetitJean F (1995) Study of inheritance of freezing tolerance in Vicia faba L. In: 2nd European conference on grain legumes, Copenhagen, Denmark, 11–15 July 1995

  • Dörffling K, Dörffling H, Lesselich G, Luck E, Zimmermann C, Melz G, Jürgens HU (1997) Heritable improvement of frost tolerance in winter wheat by in vitro-selection of hydroxyproline-resistant proline overproducing mutants. Euphytica 93:1–10

    Article  Google Scholar 

  • Gibson S, Arondel V, Iba K, Somerville C (1994) Cloning of a temperature regulated gene encoding a chloroplast w-3 desaturase from Arabidopsis thaliana. Plant Physiol 106:1615–1621

    Article  PubMed  CAS  Google Scholar 

  • Herzog H (1987a) Freezing resistance and development of faba beans as affected by ambient temperatures, soil moisture and variety. J Agron Crop Sci 159:90–100

    Article  Google Scholar 

  • Herzog H (1987b) A quantitative method to assess freezing resistance in faba beans. J Agron Crop Sci 158:195–204

    Article  Google Scholar 

  • Herzog H (1989) Influence of pre-hardening duration and dehardening temperatures on varietal freezing resistance in faba beans (Vicia faba L.). Agronomie (Paris) 9:55–61

    Google Scholar 

  • Herzog H, Olszewski A (1998) A rapid method for measuring freezing resistance in crop plants. J Agron Crop Sci 181:71–79

    Article  Google Scholar 

  • Hoffmann-Bahnsen R, Herzog H (2001) Frost resistance-screening in Lupinus albus based on chlorophyll fluorescence measurements: a methodical evaluation and adjustment. In: 4th European conference on grain legumes, Cracow, Poland, 8–12 July 2001

  • Kaup MT, Froese CD, Thompson JE (2002) A role for diacylglycerol acyltransferase during leaf senescence. Plant Physiol 129:1616–1626

    Article  PubMed  CAS  Google Scholar 

  • Koiwai A, Matsuzaki T, Suzuki F, Kawashima N (1981) Changes in total and polar lipids and their fatty acid composition in tobacco leaves during growth and senescence. Plant Cell Physiol 22:1059–1065

    CAS  Google Scholar 

  • Lea PJ, Leegood RC (1993) Plant biochemistry and molecular biology. J Wiley & Sons, Chichester, West Sussex, UK

    Google Scholar 

  • Lehninger AL (1977) Biochemistry, 2nd edn. Worth Publisher, New York

    Google Scholar 

  • Mackender RO, Leech RM (1974) The galactolipid, phospholipid, and fatty acid composition of the chloroplast envelope membranes of Vicia faba L. Plant Physiol 53:496–502

    Article  PubMed  CAS  Google Scholar 

  • McKenzie JS, Paquin R, Duke SH (1988) Cold and heat tolerance. In: Hanson AA, Barnes DK, Hill RR (eds) Alfalfa and alfalfa improvement. Agronomy monograph No. 29, ASA, WI, pp 259–302

  • Ohno R, Takumi S, Nakamura C (2001) Expression of cold-responsive Lt-Cor gene and development of freezing tolerance during cold acclimation in wheat (Triticum aestivum L.). J Exp Bot 52:2367–2374

    Article  PubMed  CAS  Google Scholar 

  • Petcu E, Terbea M (1995) Proline content and the conductivity test as screening methods for frost tolerance of winter wheat. Bulg J Plant Physiol 21:3–11

    CAS  Google Scholar 

  • Pollock CJ (1986) Fructans and the metabolism of sucrose in vascular plants. New Phytol 104:1–24

    Article  CAS  Google Scholar 

  • Rizza F, Crosatti C, Stanca AM, Cativelli L (1994) Studies for assessing the influence of hardening on cold tolerance of barley genotypes. Euphytica 75:131–138

    Article  Google Scholar 

  • Santoiani CS, Tognetti JA, Pontis HG, Salerno GL (1993) Sucrose and fructan metabolism in wheat roots at chilling temperatures. Physiol Plant 87:84–88

    Article  CAS  Google Scholar 

  • Shaner G, Finney RE (1977) The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knox wheat. Phytopathology 67:1051–1056

    CAS  Google Scholar 

  • Steel RGD, Torrie JH (1981) Principles and procedures of statistics. International student eds, McGraw-Hill, New York

    Google Scholar 

  • Taulavuori K, Taulavuori E, Sarjala T, Savonen EM, Pietiläinen P, Lähdesmäki P, Laine K (2000) In vivo chlorophyll fluorescence is not always a good indicator of cold hardiness. J Plant Physiol 157:227–229

    CAS  Google Scholar 

  • Thies W (1971) Schnelle und einfache Analysen der Fettsäurezusammensetzung in einzelnen Raps-Kotyledonen. I. Gaschromatographische und papierchromatographische Methode. Z Pflanzenzüchtung 65:181–202

    Google Scholar 

  • Thomashow MF (1999) Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annu Rev Plant Physiol Plant Mol 50:571–599

    Article  CAS  Google Scholar 

  • Utz HF (1991) A computer program for statistical analysis of plant breeding experiments. Institute of plant breeding, seed science and population genetics. University of Hohenheim

  • Vigh L, Maresca B, Harwood JL (1998) Does the membrane’s physical state control the expression of heat shock and other genes? Trends Biochem Sci 2:69–374

    Google Scholar 

  • Wei H, Dhanaraj AL, Arora R, Rowland LJ, Fu Y, Sun L (2006) Identification of cold acclimation-responsive Rhododendron genes for lipid metabolism, membrane transport and lignin biosynthesis: importance of moderately abundant ESTs in genomic studies. Plant Cell Environ 29:558–570

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by funds from the commission of the European Communities, specific Research Program Quality of Life and Management of Living Resources, QLK5-2001-02307, acronym “EUFABA”. It does not necessarily reflect its views and in no way anticipates the Commissions’ future policy in this area. The authors thank NPZ-Lembke Company for their financial contribution, and Prof. Fred Stoddard for his very valuable hints.

Author information

Authors and Affiliations

  1. Department of Crop Sciences, Georg-August University, Von-Siebold Str. 8, 37075, Gottingen, Germany

    Mustapha Arbaoui & Wolfgang Link

Authors
  1. Mustapha Arbaoui
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wolfgang Link.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arbaoui, M., Link, W. Effect of hardening on frost tolerance and fatty acid composition of leaves and stems of a set of faba bean (Vicia faba L.) genotypes. Euphytica 162, 211–219 (2008). https://doi.org/10.1007/s10681-007-9521-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-007-9521-4

Keywords

Search

Navigation