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Variation of grain iron content in a local upland rice germplasm from the village of Huai Tee Cha in northern Thailand

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Abstract

This experiment examined variation of iron (Fe) concentration in grain of local Thai rice varieties from Huai Tee Cha village, in northern Thailand. Seeds of 17 named varieties were collected from farmers in 66 accessions. Brown rice from each accession was stained individually with Perls’ Prussian blue, with IR68144 (high grain Fe) and KDML105 (low grain Fe) as checks. A wide range of grain Fe contents were found among different varieties, different seed lots with the same name and within individual accessions, notably BB, BGU and BK. The Fe concentrations in these varieties were confirmed by chemical analysis. Two accessions of each variety with most intense staining and highest average concentration of grain Fe were selected, and 20–40 seeds from each were sown for subsequent progeny testing. There was morphological variation between plants in 5 out of 6 accessions in characteristics such as pigmentation of the apiculus, pericarp and seed coat, while the sixth was externally uniform. Analysis for Fe concentration in seeds of individual lines found that the highest Fe lines were 40% to 50% higher than the lowest Fe lines. This range of grain Fe was found in all six accessions that were progeny tested, including BGU5 which was externally uniform in appearance (H′ = 0). We have shown that variation in grain Fe concentration in local Thai rice may be found between different seed lots bearing the same name and within individual seed lot as well as between varieties. This level of genetic variation may need to be considered when assessing for traits such as grain Fe for breeding programs.

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Abbreviations

Fe:

Iron

FDA:

Iron deficiency anemia

IFPRI:

International Food Policy Research Institute

WHO:

World Health Organization

AFLP:

Amplified fragment-length polymorphism

ISSR:

Inter simple sequence repeat

SSR:

Simple sequence repeat

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Acknowledgements

We acknowledge financial support from the Collaborative Crop Research Program of the McKnight Foundation and Thailand Research Fund. We thank Professor Shu Fukai for editorial suggestion and farmers of Huai Tee Cha village for making available their rice seeds for our studies. KDML105 was supplied by the Thai Rice Department, and IR68144 by IRRI.

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Authors and Affiliations

  1. Agronomy Department, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand

    Saicum Pintasen, Sansanee Jamjod & Benjavan Rerkasem

  2. School of Land, Crop and Food Sciences, The University of Queensland, St. Lucia, QLD, 4072, Australia

    Chanakan Prom-u-thai

  3. Highland Research and Training Centre, Chiang Mai University, Chiang Mai, 50200, Thailand

    Narit Yimyam

Authors
  1. Saicum Pintasen
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  2. Chanakan Prom-u-thai
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  3. Sansanee Jamjod
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  4. Narit Yimyam
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  5. Benjavan Rerkasem
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Correspondence to Chanakan Prom-u-thai.

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Pintasen, S., Prom-u-thai, C., Jamjod, S. et al. Variation of grain iron content in a local upland rice germplasm from the village of Huai Tee Cha in northern Thailand. Euphytica 158, 27–34 (2007). https://doi.org/10.1007/s10681-007-9421-7

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  • DOI: https://doi.org/10.1007/s10681-007-9421-7

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