Summary
We have previously identified 2 RAPD and 8 AFLP markers that were associated with yellow seed coat gene in a stable and pure yellow-seeded DH line No. 2127-17, however, they were not suitable in large-scale MAS. In this paper, it reported our efforts in developing rapid and reliable SCAR and CAPS markers from the RAPD and AFLP markers. Based on the sequence information in the regions of the most closely linked 4 AFLP and 2 RAPD markers, one SCAR (SCS1130) and one CAPS (SCA1) maker were successfully developed. Linkage analysis on a 127 DH lines population derived from the cross Hui5148-2 × No. 2127-17 revealed that they were tightly flanked (3.2 cM, 3.8 cM respectively). The resulting SCS1130 and SCA1 were further evaluated in backcross breeding families and demonstrated more accurate and valuable in MAS breeding. The development of these new markers would facilitate and accelerate the yellow seed coat breeding in Brassica napus.
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Abbreviations
- AFLP:
-
amplified fragment length polymorphism
- CAPS:
-
cleaved amplified polymorphic sequence
- CM:
-
centiMorgan
- CMS:
-
cytoplasmic male sterility
- DH:
-
doubled haploid
- EB:
-
ethidium bromide
- MAS:
-
marker assisted selection
- PAGE:
-
polyacrylamide gels electrophoresis
- PCR:
-
polymerase chain reaction
- RAPD:
-
random amplified polymorphic DNA
- SCAR:
-
sequence characterized amplified region
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Liu, Z., Fu, T., Wang, Y. et al. Development of SCAR and CAPS Markers for a Partially Dominant Yellow Seed Coat Gene in Brassica Napus L. Euphytica 149, 381–385 (2006). https://doi.org/10.1007/s10681-006-9084-9
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DOI: https://doi.org/10.1007/s10681-006-9084-9