Abstract
In China Polima cytoplasmic male sterility (cms) is currently the most important hybrid system used for the breeding of hybrids. In an effort to develop yellow-seeded Polima cms restorer lines, we used yellow-seeded, doubled haploid (DH) line No.2127-17 as the gene source in crosses with two elite black-seeded Polima cms R lines, Hui5148-2 and 99Yu42, which originated from our breeding programme. The inheritance of seed colour was investigated in the F2, BC1 and F1-derived DH progenies of the two crosses. Seed colour was found to be under the control of the maternal genotype and the yellow seed trait to be partially dominant over the black seed trait. Segregation analysis revealed a single gene locus for the partial dominance of yellow seed colour. Of 810 randomly amplified polymorphic DNA (RAPD) primers, 240 (29.6%) revealed polymorphisms between the parents. Of the 240 RAPD primers and 512 amplified fragment length polymorphism (AFLP) primer pairs, four RAPDs and 16 AFLP pairs showed polymorphisms between the bulks, with two RAPD and eight AFLP markers being identified in the vicinity of the seed-coat colour gene locus using a DH progeny population—derived from the cross Hui5148-2×No.2127-17—of 127 individuals in combination with the bulked segregant analysis strategy. Seven of these latter ten markers were linked to the allele for yellow seed, whereas the other three were linked to the allele for black seed. The seed-coat colour gene locus was bracketed by two tightly linked markers, EA02MG08 (2.4 cM) and S1129 (3.9 cM). The partial dominance and single gene control of the yellow seed-coat colour trait together with the available molecular markers will greatly facilitate the future breeding of yellow-seeded hybrid varieties.
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References
Ahmed SU, Zuberi MI (1971) Inheritance of seed coat color in Brassica campestris L. variety Toria. Crop Sci 11:309–310
Anand IJ, Reddy WR, Rawat DS (1985) Inheritance of seed coat color in mustard. Indian J Genet 45:34–37
Bradeen JM, Simon PW (1998) Conversion of an AFLP fragment linked to the carrot Y2 locus to a simple, codominant, PCR-based marker form. Theor Appl Genet 97:960–967
Charters YM, Robertson A, Wilkinson MJ, Ramsay G (1996) PCR analysis of oilseed rape cultivars (Brassica napus L. ssp. oleifera) using 5′-anchored simple sequence repeat (SSR) primers. Theor Appl Genet 92:442–447
Chen BY, Heneen WK (1992) Inheritance of seed color in Brassica campestris L. and breeding of yellow-seeded B.napus L. Euphytica 59:157–163
Chen BY, Heneen WK, Jonsson R (1988) Resynthesis of Brassica napus L. through interspecific hybridization between B. alboglabra bailey and B. campestris L. with special emphasis on seed color. Plant Breed 101:52–59
Getinet A, Rakow G (1997) Repression of seed coat pigmentation in Ethiopian mustard. Can J Plant Sci 77:501–505
Halldēn C, Nilsson NO, Rading IM, Säll T (1994) Evaluation of RFLP and RAPD markers in a comparison of Brassica napus breeding lines. Theor Appl Genet 88:123–128
Hawk JA (1982) Single gene control of seed color and hypocotyls color in turnip rape. Can J Plant Sci 62:331–334
Heneen WK, Brismar K (2001) Maternal and embryonal control of seed colour by different Brassica alboglabra chromosomes. Plant Breed 120:325–329
Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175
Lander E, Green P, Abrahamson J, Barlow A, Daley M, Lincoln S, Newburg L (1987) mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Li J, Shen BZ, Han JX, Gan L (1994) An effective procedure for extracting total DNA in rape. J Huazhong Agric Univ 13:521–523
Li JN, Zhang XK, Chen L, Chui C, Wang R (1998) Initial study on the genetics of seed color of yellow-seeded lines (Brassica napus L.) from different genetic sources. Chin J Oil Crop Sci 20:16–19
Lian Y, Li JN, Chen L (2003) Influence of red and blue light on seed coat color of yellow and black-seed in B. napus. Chin J Oil Crop Sci 25:21–24
Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with mapmaker/exp 3.0. Whitehead Institute Technical Report, 3rd edn. Whitehead Technical Institute, Cambridge, Mass.
Liu HL (1992) Studies on inheritance of yellow-seeded Brassica napus L. Acta Agron Sin 18:241–249
Lu GY, Yang GS, Fu TD (2004) Molecular mapping of a dominant genic male sterility gene (Ms) in rapeseed (Brassica napus L.). Plant Breed 123:262–265
Mammadov JA, Zwonitzer JC, Biyashev RM, Griffey CA, Jin Y, Steffenson BJ, Saghai MMA (2003) Molecular mapping of leaf rust resistance gene Rph5 in barley. Crop Sci 43:388–393
Meng JL, Shi SW, Gan L, Li ZY, Qu XS (1998) The production of yellow-seeded Brassica napus (AACC) through crossing interspecific hybrids of B. campestris (AA) and B. carinata (BBCC) with B. napus. Euphytica 103:329–333
Michelmore R, Paran WI, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832
Mohammad A, Irka SM, Aziz MA (1942) Inheritance of seed color in some Brassica oleiferous. Indian J Genet Breed 2:112–127
Negi MS, Devic M, Deleseny M, Lakshmikumaruan M (2000) Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selection. Theor Appl Genet 101:146–152
Rahman MH (2001) Production of yellow-seeded through interspecific crosses. Plant Breed 120:463–472
Rahman MH, Joersbo M, Poulsen MH (2001) Development of yellow-seed Brassica napus of double low quality. Plant Breed 120:473–478
Rashid A, Rakow G, Downey RK (1994) Development of yellow-seeded Brassica napus through interspecific crosses. Plant Breed 112:127–134
Sardesai N, Kumar A, Rajyashri KR, Nair S, Mohan M (2002) Identification and mapping of an AFLP marker linked to Gm7, a gall midge resistance gene and its conversion to a SCAR marker for its utility in marker aided selection in rice. Theor Appl Genet 105:691–698
Shi SW, Liu HL (1993) Induction of embryogensis through microspore culture of Brassica napus species and their interspecific and intergeneric hybrids. J Huazhong Agric Univ 12:544–550
Shrizadegan M (1986) Inheritance of seed color in Brassica napus L. Z Pflanzenzuecht 96:140–146
Somers DJ, Rakow G, Prabhu VK, Friesen KRD (2001) Identification of a major gene and RAPD markers for yellow seed coat colour in Brassica napus. Genome 44:1077–1082
Stringam GR (1980) Inheritance of seed color in turnip rape. Can J Plant Sci 60:331–335
Stringam GR, McGregor DI, Pawlowski SH (1974) Chemical and morphological characteristics associated with seed coat colour in rapeseed. In: Proc 4th Int Rapeseed Conf. Giessen, pp 99–108
Tang ZL, Li JN, Zhang XK, Chen L, Wang R (1997) Genetic variation of yellow-seeded rapeseed lines (Brassica napus L.) from different genetic sources. Plant Breed 116:471–474
Van Deynze AE, Pauls KP (1994) The inheritance of seed color and vernalization requirement in Brassica napus and using doubling haploid populations. Euphytica 74:77–83
Van Deynze AE, Beversdorf WD, Pauls KP (1993) Temperature effects on seed color in black- and yellow-seeded rapeseed. Can J Plant Sci 73:383–387
Van Deynze AE, Landry BS, Pauls KP (1995) The identification of restriction fragments length polymorphisms linked to seed color genes in Brassica napus. Genome 38:534–542
Vera CL, Woods DL (1982) Isolation of independent gene pairs at two loci for seed coat color in Brassica juncea. Can J Plant Sci 62:47–50
Vera CL, Woods DL, Downey RK (1979) Inheritance of seed coat color in Brassica juncea. Can J Plant Sci 59:635–637
Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
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This research was financed by funds from the High-tech program “863” (2003AA2071050) and “948” (2003-Q04) of China.
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Zhi-wen, L., Ting-dong, F., Jin-xing, T. et al. Inheritance of seed colour and identification of RAPD and AFLP markers linked to the seed colour gene in rapeseed (Brassica napus L.). Theor Appl Genet 110, 303–310 (2005). https://doi.org/10.1007/s00122-004-1835-1
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DOI: https://doi.org/10.1007/s00122-004-1835-1