Abstract
In order to clone sex determination genes, both differential screening of a cDNA library and differential display reverse transcription PCR (DDRT-PCR) were conducted, using apex tissues from a Korean cucumber cultivar that exhibited different sex expression responses to differing photoperiods. Two genes (CsM1 and CsM2) expressed only under specific male expression conditions were then cloned via differential screening of a cDNA library and designated CsCYR (Cucumis sativus cyclin-related) and CsCYP (C. sativus cyclophilin). Both genes appear to be single-copy genes, and both express under male-expressing SD (short-day) conditions. CsM10 (C. sativus male specific clone 10) was isolated via DDRT-PCR, and was expressed preferentially under male expression conditions. The CsM10 gene encodes a non-coding RNA, and harbors a 179bp-conserved sequence, found in three genes which have been classified as a biotic stress signal-associated non-translatable RNA (or non-coding RNA) group. The CsM10 gene is also a single-copy gene, which is localized in the telomeric region of chromosome 6, and its expression is different in different tissues, developmental stages, and photoperiods. Based on the obtained sequence structures and expression patterns of CsM10 and its homologues, we suggest that CsM10 function appears to occur at the RNA level.
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Abbreviations
- DDRT-PCR:
-
differential display reverse transcription PCR
- FISH:
-
fluorescence in situ hybridization
References
Ando, S. & S. Sakai, 2002. Isolation of an ethylene-responsive gene (ERAF16) for putative methyltransferase and correlation of ERAF16 gene expression with female flower formation in cucumber plants (Cucumis sativus L.). Physiol Plant 116: 213–222.
Ando, S., Y. Sato., S. Kamachi & S. Sakai, 2001. Isolation of a MADS-box gene (ERAF17) and correlation of its expression with the induction of formation of female flowers by ethylene in cucumber plants (Cucumis sativus L.). Planta 213: 943–952.
Andreeva, L., R. Heads & C.J. Green, 1999. Cyclophilins and their possible role in the stress response. Int J Exp Pathol 80: 305–315.
Arévalo-Rodriguez, M., M.E. Cardenas, X. Wu, S.D. Hanes & J. Heitman, 2000. Cyclophilin A and Ess1 interact with and regulate silencing by the Sin3-Rpd3 histone deacetylase. EMBO J 19: 3739–3749.
Atsmon, D. & E. Galun, 1960. Morphogenesis study of staminate, pistillate and hermaphrodite flowers Cucumis sativus L. Phytomorphology 10: 110–115.
Chou, I.T. & C.S. Gasser, 1997. Characterization of the cyclophilin gene family of Arabidopsis thaliana and phylogenetic analysis of known cyclophilin proteins. Plant Mol Biol 35: 873–892.
Erdmann, V.A., M. Szymanski, A. Hochberg, N. de Groot & J. Barciszewski, 1999. Collection of mRNA-like non-coding RNAs. Nucleic Acids Res 27: 192–195.
Erdmann, V.A., M. Szymanski, A. Hochberg, N. de Groot & J. Barciszewski, 2000. Non-coding, mRNA-like RNAs database Y2k. Nucleic Acids Res 28: 197–200.
Filipecki, M.K., H. Sommer & S. Malepszy, 1997. The MADS-box gene CUS1 is expressed during cucumber somatic embryogenesis. Plant Sci 125: 63–74.
Fuchs, E., D. Atmon & A.H. Halevy, 1977. Adventitious staminate flower formation in gibberellin treated gynoecious cucumber plants. Plant Cell Physiol 18: 1193–1201.
Galderisi, U., F.P. Jori & A. Giordano, 2003. Cell cycle regulation and neural differentiation. Oncogene 22: 5208–5219.
Gothel, S.F. & M.A. Marahiel, 1999. Peptidyl-prolyl cis-trans isomerases, a superfamily of ubiquitous folding catalysts. Cell Mol Life Sci 55: 423–436.
Grant, S., A. Houben, R. Vyskot, J. Siroky, W.H. Pan, J. Macas & H. Saedler, 1994. Genetics of sex determination in flowering plants. Dev Genet 15: 214–230.
Juarez, C. & J.A. Banks, 1998. Sex determination in plants. Curr Opin Plant Biol 1: 68–72.
Kamachi, S., H. Mizusawa, S. Matsuura & S. Sakai, 2000. Expression of two 1-aminocyclopropane-1-carboxylate synthase genes, CS-ACS1 and CS-ACS2, correlated with sex phenotypes in cucumber plants (Cucumis sativus L.). Plant Biotechnol 17: 69–74.
Kamachi, S., H. Sekimoto, N. Kondo & S. Sakai, 1997. Cloning of a cDNA for a 1-aminocyclopropane-1-carboxylate synthase that is expressed during development of female flowers at the apices of Cucumis sativus L. Plant Cell Phsiol 38: 1197–1206.
Koo, D.H., Y. Hur, D.C. Jin & J.W. Bang, 2002. Karyotype analysis of a cucumber cultivar (Cucumis sativus L. cv. Winter Long) using C-banding and bicolor FISH. Mol Cell 13: 413–418.
Lee, Y.K., C.B. Hong, Y. Suh & I.K. Lee, 2002. A cDNA clone for cyclophilin from Griffithsia japonica an phylogenetic analysis of cyclophilins. Mol Cells 13: 12–20.
Lu, K.P., S.D. Hanes & T. Hunter, 1996. A human peptidylprolyl isomerase essential for regulation of mitosis. Nature 380: 544–547.
Malepszy, S. & K. Niemirowicz-Szczytt, 1991. Sex determination in cucumber (Cucumis sativus) as a model system for molecular biology. Plant Sci 80: 39–47.
Marks, A.R., 1996. Cellular functions of immunophilins. Physiol Rev 76: 631–649.
Matsunaga, S., W. Uchida & S. Kawano, 2004. Sex-specific cell dividion during development of unisexual flowers in the dioecious plant Silene latifolia. Plant Cell Physiol 45: 795–802.
Murray, M.G. & W.F. Thompson, 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acid Res 8: 4321–4325.
Nitsch, J.P., E.B. Kurtz, J. Livermant & F.W. Went, 1952. The development of sex expression in cucurbit flower. Amer J Bot 39: 32–43.
Nuc, K., P. Nuc & R. Slomski, 2001. Yellow lupine cyclophilin transcripts are highly accumulated in the nodule meristem zone. Mol Plant Microbe Interact 14: 1384–1394.
Nugent, J.H., C.E. Alfa, T. Young & J.S. Hyams, 1991. Conserved structural motifs in cyclins identified by sequence analysis. J Cell Sci 99: 669–674.
Perl-Treves, R., A. Kahana, N. Rosenman, Y. Xiang & L. Silberstein, 1998. Expression of multiple AGAMOUS-like genes in male and female flowers of cucumber (Cucumis sativus L.). Plant Cell Phsiol 39: 701–710.
Pierce, K.K. & T.C. Wehner, 1990. Review of genes and linkage group in cucumber. HortSci 25: 605–615.
Przybecki, Z., M.E. Kowalczyk, E. Siedlecka, E. Urabanczyk-Wochniak & S. Malepszy, 2003. The isolation of cDNA clones from cucumber (Cucumis sativus L.) floral buds coming from plants differing in sex. Cell Mol Biol Lett 8: 421–438.
Rudich, J., A.H. Halevy & N. Kedar, 1969. Increase in femaleness of three cucurbits by treatment with ethrel, an ethylene-releasing compound. Planta 86: 69–76.
Saito, T., K. Tadakuma, N. Takahashi, H. Ashida, K. Tanaka, M. Kawamukai, H. Matsuda & T. Nakagawa, 1999a. Two cytosolic cyclophilin genes of Arabidopsis thaliana differently regulated in temporal- and organ-specific expression. Biosci Biotechnol Biochem 63: 632–637.
Saito, T., Y. Niwa, H. Ashida, K. Tanaka, M. Kawamukai, H. Matsuda & T. Nakagawa, 1999b. Expression of a gene for cyclophilin which contains an amino-terminal endoplasmic reticulum-targeting signal. Plant Cell Physiol 40: 77–87.
Sambrook, J. & D.W. Russell, 2001. Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Press, Cold Spring Harbor, New York.
Sykes, K., M.J. Gething & J. Sambrook, 1993. Proline isomerases function during heat shock. Proc Natl Acad Sci 90: 5853–5857.
Szymański, M., V.A. Erdmann & J. Barciszewski, 2003. Noncoding regulatory RNAs database. Nucleic Acids Res 31: 429–431.
Tanurdzic, M. & J.A. Banks, 2004. Sex-determining mechanisms in land plant. Plant Cell 16: S61–S71.
Taylor, C.B. & P.J. Green, 1995. Identification and characterization of genes with unstable transcripts (GUTs) in tobacco. Plant Mol Biol 28: 27–38.
Teramoto, H., T. Toyama, G. Takeba & H. Tsuji, 1996. Noncoding RNA for CR20, a cytokinin-repressed gene of cucumber. Plant Mol Biol 32: 797–808.
Theissen, G., A. Becker, A.D. Rosa, A. Kanno, J.T. Kim, T. Münster, K.U. Winter & H. Saedler, 2000. A short history of MADS-box genes in plants. Plant Mol Biol 42: 115–149.
Trebitsh, T., J.E. Staub & S.D. O'Neill, 1997. Identification of a 1-aminocyclopropane-1-carboxylic acid synthase gene linked to the female (F) locus that enhances female sex expression in cucumber. Plant Physiol 113: 987–995.
Wang, G., H. Kong, Y. Sun, X. Zhang, W. Zhang, N. Altman, C.W. de Pamphilis & H. Ma, 2004. Genome-wide analysis of the cyclin family in Arabidopsis and comparative phylogenetic analysis of plant cyclin-like proteins. Plant Physiol 135: 1084–1099.
Yamasaki, S., N. Fujii & H. Takahashi, 2000. The ethylene-regulated expression of CS-ETR2 and CS-ERS genes in cucumber plants and their possible involvement with sex expression in flowers. Plant Cell Physiol 41: 608–616.
Yamasaki, S., N. Fujii, S. Matsuura, H. Mizusawa & H. Takahashi, 2001. The M locus and ethylene-controlled sex determination in andromonoecious cucumber plants. Plant Cell Physiol 42: 608–619.
Yin, T. & J.A. Quinn, 1995. Tests of a mechanistic model of one hormone regulating both sexes in Cucumis sativus (Cucurbitaceae). Amer J Bot 82: 1537–1546.
Ylstra, B. & S. McCormick, 1999. Analysis of mRNA stabilities during pollen development ad in BY2 cells. Plant J 20: 101–108.
Zuker, M., D.H. Mathews & D.H. Turner, 1999. Algorithms and Thermodynamics for RNA Secondary Structure Prediction: A Practical Guide in RNA Biochemistry and Biotechnology, In: Barciszewski, J. & B.F.C. Clark (Eds.) NATO ASI Series, Kluwer Academic Publishers.
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Cho, J., Koo, DH., Nam, YW. et al. Isolation and characterization of cDNA clones expressed under male sex expression conditions in a monoecious cucumber plant (Cucumis sativus L. cv. Winter Long). Euphytica 146, 271–281 (2005). https://doi.org/10.1007/s10681-005-9023-1
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DOI: https://doi.org/10.1007/s10681-005-9023-1