Abstract
The CR20 gene was identified as a cytokinin-repressed gene in excised cotyledons of cucumber. We determined the sequences of some CR20 cDNAs with different structures and sequenced genomic clones for CR20. This gene consisted of three exons, and there were at least three types of transcript, which seemed to be generated by alternative splicing of the second intron. None of the CR20 transcripts included a long open reading frame (ORF). We isolated a cDNA of Arabidopsis thaliana with cucumber CR20 cDNA as a probe. This cDNA for a gene designated AtCR20-1 also lacked a long ORF. A region of 180 nucleotides was conserved in the CR20 RNA of cucumber and the AtCR20-1 RNA of Arabidopsis, although the homology was relatively low when the entire sequences were compared. Each conserved region consisted of seven elements, and seems to form stable secondary structure. These suggest that CR20 RNA may function as an RNA that is not translated into a protein.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Askew DS, Li J, Ihle JN: Retroviral insertions in the murine His-1 locus activate the expression of a novel RNA that lacks an extensive open reading frame. Mol Cell Biol 14: 1743–1751 (1994).
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K: Primer extension. In: Current Protocols in Molecular Biology, vol. 1, pp. 4.8.1–4.8.5. Greene Publishing Associates/Wiley-Interscience, New York (1991).
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K: Size fractionation using sucrose gradients. In: Current Protocols in Molecular Biology, vol. 1, pp. 5.3.2–5.3.8. Greene Publishing Associates/Wiley-Interscience, New York (1991).
Brannan CI, Dees EC, Ingram RS, Tilghman SM: The product of the H19 gene may function as an RNA. Mol Cell Biol 10: 28–36 (1990).
Brockdorff N, Ashworth A, Kay GF, McCabe VM, Norris DP, Cooper PJ, Swift S, Rastan S: The product of the mouse Xist gene is a 15 kb inactive X-specific transcript containing no conserved ORF and located in the nucleus. Cell 71: 515–526 (1992).
Brown CJ, Hendrich BD, Rupert JL, Lafrenière RG, Xing Y, Lawrence J, Willard HF: The human XIST gene: analysis of a 17 kb inactive X-specific RNA that contains conserved repeats and is highly localized within the nucleus. Cell 71: 527–542 (1992).
Crane KE, Ross CW: Effects of wounding on cytokinin activity in cucumber cotyledons. Plant Physiol 82: 1151–1152 (1986).
Crespi MD, Jurkevitch E, Poiret M, d'Aubenton-Carafa Y, Petrovics G, Kondorosi E, Kondorosi A: enod40, a gene expressed during nodule organogenesis, codes for a non-translatable RNA involved in plant growth. EMBO J 13: 5099–5112 (1994).
Fickett JW: Recognition of protein coding regions in DNA sequences. Nucl Acids Res 10: 5303–5318 (1982).
Fletcher RA, McCullagh D: Benzyladenine as a regulator of chlorophyll synthesis in cucumber cotyledons. Can J Bot 49: 2197–2201 (1971).
Fletcher RA, Teo C, Ali A: Stimulation of chlorophyll synthesis in cucumber cotyledons by benzyladenine. Can J Bot 51: 937–939 (1973).
Hall RH: Cytokinins as a probe of developmental processes. Annu Rev Plant Physiol 24: 415–444 (1973).
Hao Y, Crenshaw T, Moulton T, Newcomb E, Tycko B: Tumour-suppressor activity of H19 RNA. Nature 365: 764–767 (1993).
Ohya T, Suzuki H: Cytokinin-promoted polyribosome formation in excised cucumber cotyledons. J Plant Physiol 133: 295–298 (1988).
Pachnis V, Brannan CI, Tilghman SM: The structure and expression of a novel gene activated in early mouse embryogenesis. EMBO J 7: 673–681 (1988).
Sambrook J, Fritsch EF, Maniatis T: Sequencing techniques and strategies. In: Molecular Cloning: A Laboratory Manual, 2nd ed., vol. 2, pp. 13.3–13.10. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Teramoto H, Toyama T, Takeba G, Tsuji H: Changes in expression of two cytokinin-repressed genes, CR9 and CR20, in relation to aging, greening and wounding in cucumber. Planta 196: 387–395 (1995).
Teramoto H, Momotani E, Takeba G, Tsuji H: Isolation of a cDNA for a cytokinin-repressed gene in excised cucumber cotyledons. Planta 193: 573–579 (1994).
Toyama T, Teramoto H, Takeba G, Tsuji H: Cytokinin induces a rapid decrease in the levels of mRNAs for catalase, 3-hydroxy-3-methylglutaryl CoA reductase, lectin and other unidentified proteins in etiolated cotyledons of cucumber. Plant Cell Physiol 36: 1349–1359 (1995).
Uheda E, Kuraishi S: Increase of cytokinin activity in detached etiolated cotyledons of squash after illumination. Plant Cell Physiol 18: 481–483 (1977).
Velleca MA, Wallace MC, Merlie JP: A novel synapse-associated noncoding RNA. Mol Cell Biol 14: 7095–7104 (1994).
Watanabe Y, Yamamoto M: S. pombe mei2+ encodes an RNA-binding protein essential for premeiotic DNA synthesis and meiosis I, which cooperates with a novel RNA species meiRNA Cell 78: 487–498 (1994).
Yoshida H, Kumimoto H, Okamoto K: dutA RNA functions as an untranslatable RNA in the development of Dictyostelium discoideum. Nucl Acids Res 22: 41–46 (1994).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Teramoto, H., Toyama, T., Takeba, G. et al. Noncoding RNA for CR20, a cytokinin-repressed gene of cucumber. Plant Mol Biol 32, 797–808 (1996). https://doi.org/10.1007/BF00020478
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00020478