Abstract
This study was performed in order to check the applicability of a protocol for somatic embryogenesis in Cyclamen persicum and to compare the in vitro response of cultivars belonging to different cyclamen growth types and flower colours obtained from two breeders. One hundred ovules per plant were prepared from 32 tested F1 hybrid cultivars represented by five plants each. Callus induction monitored after 8 weeks varied between 42 and 85%. Variation occurred between replications and single plants of a cultivar. Consistency and colour of the developing calluses were diverse. Only in 1 cultivar out of the 32 tested no regeneration of somatic embryos was obtained. The regeneration rates varied from 0 to 65% among cultivars. In the group of midi-type cultivars the regeneration response was lower than that in mini and giganteum types. High regeneration rates were observed in cultivars with flamed flowers.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- 2iP:
-
6-(γ,γ-dimethylallylamino)purine
References
Bongartz, W., 1999. Cyclamen. Thalacker-Medien, Braunschweig, pp. 42–50.
Dillen, W., I. Dijkstra & J. Oud, 1996. Shoot regeneration in long-term callus cultures derived from mature flowering plants of Cyclamen persicum {Mill.} Plant Cell Rep 15: 545–548.
Fukui, H., T. Yamamoto, T. Asano & M. Nakamura, 1988. Effect of plant growth regulators on in vitro organogenesis of cyclamen (Cyclamen persicum {Mill.}). Res Bull Fac Agric Gifu Univ 53: 139–145.
Hohe, A., T. Winkelmann & H.-G. Schwenkel, 1999a. CO2 accumulation in bioreactor suspension cultures of Cyclamen persicum Mill. and its effect on cell growth and regeneration of somatic embryos. Plant Cell Rep 18: 863–867.
Hohe, A., T. Winkelmann & H.-G. Schwenkel, 1999b. The effect of oxygen partial pressure in bioreactors on cell proliferation and subsequent differentiation of somatic embryos of Cyclamen persicum Mill. Plant Cell Tiss Org Cult 59: 39–45.
Hohe, A., T. Winkelmann & H.-G. Schwenkel, 2001. Development of somatic embryos of Cyclamen persicum Mill. in liquid culture. Gartenbauwissenschaft 66: 219–224.
Kiviharju, E., U. Tuominen & T. Törmälä, 1992. The effect of explant material on somatic embryogenesis of Cyclamen persicum Mill. Plant Cell Tiss Org Cult 28: 187–194.
Kreuger, M., E. Postma, Y. Brouwer & G.-J. van Holst, 1995. Somatic embryogenesis of Cyclamen persicum in liquid medium. Physiol Plant 94: 605–612.
Murashige, T. & F. Skoog, 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473–497.
Ockendon, D.J., 1985. Anther culture in Brussels sprouts (Brassica oleracea var.gemmifera) II. Effect of genotype on embryo yields. Ann Appl Biol 107: 101–104.
Ockendon, D.J. & R.A. Sutherland, 1987. Genetic and non-genetic factors affecting anther culture of Brussels sprouts (Brassica oleracea var.gemmifera) Theor Appl Genet 74: 566–570.
Otani, M. & T. Shimada, 1991. Somatic embryogenesis and plant regeneration from Cyclamen persicum Mill. leaf cultures. Plant Tiss Cult Lett 8: 121–123.
Püschel, A.K., 2000. Zur genetischen Analyse der Kallusbildung und somatischen Embryogenese in vitro bei Cyclamen persicum Mill. PhD Thesis, University of Hannover.
Püschel, A.K., H.-G. Schwenkel & T. Winkelmann, 2003. Inheritance of the ability for regeneration via somatic embryogenesis in Cyclamen persicum Mill. Plant Cell Tiss Org Cult 72: 43–51.
Schween, G. & H.-G. Schwenkel, 2003. Effect of genotype on callus induction, shoot regeneration, and phenotypic stability of regenerated plants in the greenhouse of Primula spec. Plant Cell Tiss Org Cult 72: 53–61.
Schwenkel, H.-G. & T. Winkelmann, 1998. Plant regeneration via somatic embryogenesis from ovules of Cyclamen persicum Mill. Plant Tiss Cult Biotechnol 4: 28–34.
Takamura, T. & M. Tanaka, 1996. Somatic embryogenesis from the etiolated petiole of cyclamen (Cyclamen persicum Mill.). Plant Tiss Cult Lett 13: 43–48.
Takamura, T. & I. Miyajima, 1997. Micropropagation of Cyclamen persicum Mill. In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry, Vol. 40, High-tech and Micropropagation IV. Springer, Berlin Heidelberg, pp. 96–112.
Takamura, T., I. Miyajima & E. Matsuo, 1995. Somatic embryogenesis of Cyclamen persicum Mill. ‘Anneke’ from aseptic seedlings. Plant Cell Rep 15: 22–25.
Wicart, G., A. Muras & A. Lutz, 1984. Histological study of organogenesis and embryogenesis in Cyclamen persicum { Mill.} tissue cultures: Evidence for a single organogenic pattern. Protoplasma 119: 159–167.
Winkelmann, T., A. Hohe & H.-G. Schwenkel, 1998. Establishing embryogenic suspension cultures in Cyclamen persicum ‘Purple Flamed’. Adv Hort Sci 12: 25–30.
Winkelmann, T., L. Meyer & M. Serek, 2004a. Desiccation of somatic embryos of Cyclamen persicum Mill. J Horticult Sci Biotechnol 79(3): 479–483.
Winkelmann, T., L. Meyer & M. Serek, 2004b. Germination of encapsulated somatic embryos of Cyclamen persicum. HortScience 39: 1093–1097.
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Winkelmann, T., Serek, M. Genotypic differences in callus formation and regeneration of somatic embryos in Cyclamen persicum Mill. Euphytica 144, 109–117 (2005). https://doi.org/10.1007/s10681-005-5038-x
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DOI: https://doi.org/10.1007/s10681-005-5038-x