To highlight the importance of integrating LCA in the early phases of research and development of a product or process, first-hand experiences from two different case studies in the food and feed sector will be described. Each of the case studies discussed in this paper is at the pilot scale, or beginning phases of industrial pilot plans, by which LCA is key to ensuring reduced ecological effects. The case studies are (a) life cycle assessment of fish feed derived from plants and insects and (b) life cycle assessment of plant -based protein products for the food value chain. An introduction from LCA perspective is given in Table 1. More information, especially related to the LCA stage “Interpretation”, can be found in the sections below.
Data were mainly gathered from primary sources, i.e., farmers, research institutes, food/feed processors and food/feed factories, acting as project partners in both R&D case studies. Where primary data were absent, these were substituted by secondary data, i.e., Ecoinvent or literature (scientific paper, patents, industry data). Other types of data substitutes could be simulation/ thermodynamic modelling or stoichiometric calculations, see also Beemsterboer et al. (2020). The data sources were documented accordingly in each case to ensure transparency and comparability, but some of them are not yet publicly accessible due to IPR.
Both case studies consider the use of waste streams, which are produced as well as partially consumed within the system boundary. Allocating the environmental impacts were avoided at this stage. This is due to the fact, that most of the waste streams used or produced have no economic value so far, i.e., are defined as “waste”. Secondly, by allocating the whole ecological backpack to the desired product, a conservative or even “worst case” scenario is considered.
Case study 1: life cycle assessment of fish feed derived from plants and insect
The global demand for fish and meat is still growing (Chemnitz and Becheva 2021; FAO 2020). The importance of the impacts of agricultural practices on water and land use, climate change, and environmental degradation, such as eutrophication, and terrestrial and marine acidification, is well acknowledged and has been exhibited by many studies. In this context, within the last years, insects are repeatedly discussed as a future-oriented, sustainable source of protein for the food industry, as the ecological, economic, physiological, and ethical advantages outweigh those of meat. In aquacultural systems, insects are also gaining interest as feed to provide a sustainable alternative to the fishmeal paradox (Alfiko et al. 2021; Silva et al. 2009), whose production leads to high consumption of resources and negative environmental impacts. Reducing the proportion of fish protein in favor of insect proteins in combination with vegetable feed components has the potential to reduce environmental burdens (Zanten et al. 2014). Within the scope of the project discussed here, the fish feed was produced from Hermetia illucens larvae and Lemna minor in an inline recirculating aquaponics model for urban sites, optimized, and scaled up, which couples waste and environmental service concepts in one production system efficiently. Lemna minor is also used for other purposes, such as wastewater treatment (O’Neill et al. 2020), but we intended to use it as an active ingredient for a circular-based production system. At the same time, the value chain produces high-quality, market-accessible raw materials for the food and feed industry. All research activities were accompanied by LCA as well as cost analyses to measure and compare ecological and economic effects to finally result in sustainable alternatives (Goyal et al. 2021).
The analysis of aquafeed was carried out cradle-to-gate, i.e., from the cradle (exploration of the raw materials) to the production of the individual components for the manufacture and use of the compound feed. Here, the pellets produced are referred to as LH pellets, which were found to have a similar protein and fat content as conventional tilapia feed. The system boundary considered in this study is shown in Fig. 3. The energy utilized in this study was derived from a biogas plant that was installed near the container where the species were rearing. This filled in as a benefit according to the environmental impacts’ perspective.
Data were collected mainly from the process engineers using designated surveys with questions regarding inputs (raw material, energy, equipment) and the outputs (products and by-products), while additional data were also considered from the composition datasheet (e.g., for disinfectants, fertilizer). For primary data, which was unavailable, information based on literature was used as a proxy. Life cycle inventory data were, if not available, substituted or modeled (retrosynthetic break-down (Ott et al. 2016)), if possible. To cope with uncertainties, we finally also included a sensitivity analysis, concentrating on the calculation of upper and lower limits, the “worst cases”.
The modeling of the material and energy flows was carried out with the help of GaBi (developed by Sphera Solutions GmbH, Germany). By using the integrated Ecoinvent (version 3.5) database (Wernet et al. 2016), corresponding inventory data concerning the supply of raw materials, energy, or transport processes were integrated. The emission factors of all materials, energy sources, and transport processes are based on the Ecoinvent database. The impact assessment was carried out according to ReCiPe 2016 v1.1, utilizing midpoint indicators at the hierarchical level (Huijbregts et al. 2016). The time horizon of the impact assessment was 100 years. The ISO standards defined a functional unit (FU) as a quantified performance of a product system to be used as the reference unit in an LCA study. The use of mass-based FU was considered by Iribarren et al. 2012 for analysing the environmental footprint of marine aquafeed and also by Rustad 2016 to analyse insect meal which had the potential to replace fish meal. Hence, 1 kg LH pellets was considered as the FU for the LCA of fish feed production.
Figures 4 and 5 show the reduction potential of greenhouse gas (GHG) emissions for the supply of individual ingredients, i.e., energy and resources, for raw material production. Greenhouse Gas Potential, also referred to as Climate Change or Carbon Footprint, was chosen as it is the most prominent key objective for political and business-related decisions and can be seen as an important screening indicator of the environmental impact of products and processes. Additionally, as climate change is not reflecting the wholistic environmental profile of products and processes, we generally recommend selecting further impact categories for screening purposes as well, depending on the scope of the study. To give an example, in the context of the production of alternative feed, land use, primary energy demand, and water footprint could be of particular importance. The results to other impact categories can be found in (Goyal et al. 2021). Results are shown relatively, i.e., normalized to the worst-case value, which represents the starting point of the investigation. This prevents premature comparisons with other studies and conclusions, which can be the subject of later, more detailed analysis.
Figure 4 shows the development process of insect breeding. Firstly, insects were fed with cereal bran (Stage 1 in Fig. 4), affecting the ecological impact by more than 50%, and initiating to use of organic waste as feedstock (Stage 2 in Fig. 4), according to (EU) 2017/893) (European Commission 2017). Consequently, another driver could be identified, namely the energy consumption for maintaining the temperature inside the breeding container. The heat used is taken from a biogas plant which is installed in the vicinity of the container where insects are rearing. This is a clear advantage from the perspective of environmental impacts compared to the heat generated by non-renewable sources of energy Additionally, if the energy arising in the biogas plant would be considered as “waste energy” (as of now no dedicated purpose of it was foreseen at the manufacturing site), the environmental burden could be further reduced (Stage 3 in Fig. 4), revealing new ecological drivers such as cleaning agents, sawdust used for breeding, and electricity from the biogas plant. Hence, although optimization of 75% could be achieved, there is still potential for even further optimization, which will be considered during the upscaling stage.
Figure 5 shows the results for Leman minor process optimization through ecological screening. As for the rearing, a lot of technical equipment is needed, and these components (e.g., pipes, filters, trays, pumps, lamps, dehumidifiers) were listed as precisely as possible according to the material/equipment list given by the company which was establishing the rearing container. An initial evaluation based on the yield of Lemna minor culture obtained without optimization (Stage 1 in Fig. 5), however, showed inacceptable high ecological drawbacks not justifying the Lemna minor cultivation. Based on this, key performance criteria and recommendations were defined to approach at least ecological comparability to standard fish feed components. In the beginning, these key metrics could only be obtained by using artificial fertilizer instead of the use of feces from Hermetia illucens cultivation. As a consequence, and due to its significant ecological impact, the contribution analysis shows its greater impact. Its optimization, either by reducing its consumption (first trials were made empirically), recirculating the nutrient-rich water, or optimizing the feces composition, is an urgent subject of future investigation to reduce the overall environmental burdens of Lemna minor rearing. Secondly, electricity consumption was identified to be a main ecological driver, mainly attributable to air-conditioning, water pump circuits, and lighting. Also, in this case, the coupling to renewable resources, in this case, taken from the biogas plant (Stage 2 in Fig. 5), could result in a drastic change in the relative contribution of environmental impacts by a factor of 2.
Several recommendations and strategies could be derived for the production of sustainable and innovative raw materials for fish feed supply. Cultivation of Hermetia illucens and Lemna minor demand feed and energy, the main ecological drivers. The use of secondary raw materials can decrease the environmental burden by up to 75%. It is therefore important to perform LCA at the early development phase of a product or service to provide the stakeholders involved in the scale-up process with information regarding the influence of single parameters as well as the importance of using renewable energy and secondary raw materials for industrial production. Also, the impacts of the raw materials are enlightened by the successful implementation of LCA from the very beginning of the project.
The results concluded that fish feed made from Lemna minor and Hermetia illucens is potentially more sustainable than standard feed. The results were not comparable with literature because of the scale of the study but plant-based feed is more environmentally friendly than conventional feeds having high amount of fish remains or wild fish (Samuel-Fitwi et al. 2013; Smarason et al. 2017).
Case study 2: life cycle assessment of plant and fungal-based protein products for the sustainable food value chain
Animal-derived protein contributes significantly to the production of greenhouse gases, intensifies pressure on land use, and can have negative health consequences (Godfray et al. 2018; Song et al. 2016). In 2016, European Union used 39% of the total land area for agricultural production (Eurostat 2021) while the majority of agricultural land is used to produce livestock, either for feed production or grazing, with increasing competitive pressure from feedstock demand for non-food applications such as biofuels. The increasing demand for food proteins can be met by the utilization of proteins from alternative and new sources which includes under-explored legumes, protein crops, and fungi as well as side streams from food processing. In general, plant-based diets or meat substitutes often result in lower impacts on the environment than those with meat, see, e.g., (de Boer et al. 2014; Pimentel and Pimentel 2003; Westhoek et al. 2014). So far, existing LCA studies of food indicate the importance of side stream utilization on the overall sustainability (Nijdam et al. 2012; Scherhaufer et al. 2020; Souza Filho et al. 2019), but they also focus on the development of environmentally sustainable handling of different food side-flows in the future, as the processing and utilization of waste-stream do not automatically result in ecological benefits (Scherhaufer et al. 2020). Additionally, following the growing transition from animal-derived protein to plant-based protein, it is important to explore different plant-sourced proteins (e.g., lentil, soy, pea) and compare them in terms of their ecological, as well as their economic sustainability. As the price of food products is considered one of the key factors influencing consumers’ purchase behavior of environmentally sustainable foods (Vermeir et al. 2020), it is imperative to have a reasonable price for new products (Katt and Meixner 2020). Consequently, insights into the production costs of new products and the competing products are also needed (Rivera and Azapagic 2016).
The EU-funded Horizon 2020 project, Smart protein, aims to develop protein products from plants, including fava beans, lentils, chickpeas, and quinoa—with a focus on improving their structure, taste, and flavor, but also strongly focuses on sustainable agricultural processes, the utilization of by-products and residues as well as ingredients that are usually used for animal feed (Smart Protein 2020). In addition to plant-based proteins, microbial biomass proteins are created from edible fungi by upcycling side streams from pasta (pasta residues), bread (bread crusts), and beer production (spent yeast and malting rootlets). By using LCA, but also economic (life cycle costing) and stakeholder analyses, Smart Protein will be able to benchmark the findings against conventional protein food and agriculture approaches to evaluate its potential degree of competitiveness, sustainability, and resilience, and thus pave the way for enhanced protein production from plant and plant-based products and encourage their uptake, in Europe.
The determination of the ecological, but also the economic impact of plant protein products requires the implementation of upstream processes as well. Within Smart Protein new and organic agricultural practices and processing techniques will be developed (for which no or hardly any life cycle inventory data is yet available). Therefore, the collection of existing data was started to get a first insight into the sustainability of crops. In particular, lentils are of greater interest because lentil protein isolates have comparable protein content compared to dairy and soy-based milk products. It also possesses good sensory and techno-functional properties (Jeske et al. 2019). All these characteristics motivated the further investigation of the suitability of lentil protein isolate as a base material for a novel alternative to dairy or soy products.
A simplified LCA approach has been used to produce initial results. The goal was to explore the environmental benefits of using an alternative plant-based product. The considered data came from different literature sources as mentioned within because of its limited availability following a streamlined LCA approach. The advantage of such screening is that it highlights hotspots of environmental concerns which could be used for product development/commercialization (Todd et al. 1999). Berardy et al. determined the climate change potential of soy protein isolate at 20.2 kg CO2 equivalents per kg of soy protein isolate (Berardy et al. 2015). Similarly, the climate change potential for soybeans and soymeal is about 0.6 kg CO2 equivalents (per kg of soybeans) and 0.7 kg CO2 equivalents (per kg of soymeal), respectively (Dalgaard et al. 2007). Braun et al. compared the ecological impact to other protein sources such as concentrated whey protein with 16 kg CO2 equivalents per kg protein and skimmed milk powder with 23 kg CO2 equivalents per kg protein (Braun et al. 2016). In contrast, the climate change potential for lentil protein isolate ranges between 3.5 and 4.2 kg CO2 equivalents per kg isolate (Alonso-Miravalles et al. 2019). Looking at the costs, in general, it can be concluded that plant-based proteins could be a low-cost alternative to animal-based proteins in the food industry. The cost of cow milk protein is found to be higher than those of plant-based protein, such as soy (Li et al. 2019). Also, at the market price level, soy-based cheese is cheaper than milk-based cheese (Jeewanthi and Paik 2018). Lentils might also be another potentially valuable alternative for animal-based products. According toresearch (Chaudhary and Tremorin 2020), for example, the partial substitution of beef with Canadian lentils in a burger leads to a decrease in market price. The level of price reduction, however, depends on many factors such as geographic location, farming practice, and processing method (Khazaei et al. 2019).
Figure 6 presents the first comparable screening of environmental impacts and farm gate prices of lentils compared to soy and cow-milk-based protein supply. The ecological impact of lentil-based proteins, related to Climate Change potential, is promising compared to soy and milk-based proteins. This is not only relevant to climate change, but also to other LCA impact categories (LCIA) as well, as shown by Alonso-Miravalles et al. (2019). Their LCA study showed that the principal driver for the ecological impact of lentil protein isolates is the cultivation of the lentils, thus having ecological burdens, especially in the LCIA categories of Land Use and Aquatic Eutrophication. This first screening encourages to start the development of a lentil-based yogurt alternative, which could pose a viable way towards increasing human lentil consumption in Western countries, produced sustainably, regionally, or even locally (Boeck et al. 2021). Considering the similarity of the production process of yogurt from different protein isolates, one could expect that yogurt derived from lentil protein isolate might have a lower ecological burden than dairy- and soy-based products.
As a result of increased yields and acreage expansion, lentil production has increased globally since the 1950s. However, in Europe, the area under lentils declined sharply in the 1990s, with about 65% of the world's lentil production coming from Canada and India. Only in the last few years lentil cultivation has experienced a revival. The largest producers in Europe are Spain and France (FAO 2019). Due to limited own production and high import rates, the price is comparably high. Based on data from the Food and Agricultural Organization of the United Nations (FAO), farm gate prices for the year 2019 in Europe for soybeans were estimated to range between 284.1 and 592.7 $/tonne (FAO 2021). For cow milk and lentils, the farm gate price was, respectively, between 301.1 and 715.1 $/tonne and between 506.9 and 1799.4 $/tonne (FAO 2021). In 2019, soybeans were slightly less expensive in comparison to cow’s milk, while European lentils were significantly more expensive (Fig. 6).
In the future, long-term optimization of agricultural practices is expected to result not only in a further reduction of the environmental impacts but also will affect the economic profile, thus reducing the high European farm gate prices of lentils as well.
This is currently addressed by the Smart Protein project: on the one hand, agricultural optimization activities are taking place, which is also strongly needed to start project-related ecological as well as the economic screening of lentil cultivation in comparison to benchmarks. Therefore, a project-specific survey was established and is currently being evaluated. On the other hand, the processing of lentils will result in side streams, which are foreseen to utilize as well to further reduce the environmental impact of lentil cultivation and isolate processing, but also lower the final lentil-based product costs. Allocation and sensitivity analyses will be then performed accordingly.