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Decontamination of anthracene-polluted soil through white rot fungus-induced biodegradation

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Abstract

This study investigated the effect of inoculation of white rot fungus, Pleurotus ostreatus, incubation time, and levels of contamination on anthracene degradation in contaminated soil over a 90-day period. Control samples were set up to compare rates of degradation at different levels of anthracene contamination in soil. Using HPLC, extracts from soil were analyzed on intervals of 30 days to determine the level of anthracene remaining in soil. After 90 days of incubation, it was observed that time, level of contamination, and fungal treatment affected the rate of degradation of all levels of anthracene contamination, inoculated soil showed more degradation of anthracene (76–89%) compared to control soil (33–51%). It was also observed that concentration of the anthracene increased at different sampling dates in some of the soil samples. In this study, such increase in anthracene concentration was attributed to errors of extraction, sorption of the anthracene to soil particles, and possible repolymerization of anthracene in soil organic matter. The release of ligninolytic enzymes such as lignin peroxidase, laccase, and manganese peroxidase by Pleurotus ostreatus was associated with the anthracene degradation observed. A general observation indicates that an interaction between time, level of contamination, and soil treatment is vital in the degradation of high levels of anthracene contamination by the white rot fungus.

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References

  • Amellal N, Portal JM, Berthelin J (2001) Effects of soil structure on the bioavailability of polycyclic aromatic hydrocarbons within aggregates of a contaminated soil. Appl Geochem 16:1611–1619

    Article  CAS  Google Scholar 

  • Arora DS, Chander M, Gill PK (2002) Involvement of lignin peroxidase, manganese peroxidase and laccase in degradation and selective ligninolysis of wheat straw. Int Biodeterior Biodegradation 50:115–120

    Article  CAS  Google Scholar 

  • Atanassova I, Brummer GW (2004) Polycyclic aromatic hydrocarbons of anthropogenic and biopedogenic origin in a colluviated hydromorphic soil of Western Europe. Geoderma 120:27–34

    Article  CAS  Google Scholar 

  • Baars B (2002) The wreckage of the oil tanker ‘Erika’—human health risk assessment of beach cleaning, sunbathing and swimming. Toxicol Lett 128:55–68

    Article  CAS  Google Scholar 

  • Baar DP, Aust SD (1994) Conversion of lignin peroxidase 111 to active enzyme by cation radicals. Arch Biochem Biophys 1:511–515

    Article  Google Scholar 

  • Bakker MI, Casado B, Koerselman JW, Tolls J, Kolloffel C (2000) Polycyclic aromatic hydrocarbons in soil and plant samples from the vicinity of an oil refinery. Sci Total Environ 263:91–100

    Article  CAS  Google Scholar 

  • Bogan BW, Sullivan WR (2003) Physicochemical soil parameters affecting sequestration and mycobacterial biodegradation of polycyclic aromatic hydrocarbons in soil. Chemosphere 52:1717–1726

    Article  CAS  Google Scholar 

  • Bonnet M, Schantz B, Gerstl Z (2005) An independent prediction of the effect of dissolved organic matter on the transport of polycyclic aromatic hydrocarbons. J Contam Hydrol 45:157–180

    Google Scholar 

  • Boonchan S, Britz ML, Stanley GA (2000) Degradation and mineralization of high molecular weight polycyclic aromatic hydrocarbons by defined fungal-bacterial cocultures. Appl Environ Microbiol 66:1007–1019

    Article  CAS  Google Scholar 

  • Bouchard T, Lam C, Goalen B (2004) Oxidation of polycyclic aromatic hydrocarbons (PAH) by white rot fungus, Trametez versicolor. Enzyme Microb Technol 31:3–9

    Google Scholar 

  • Breedveld S, Sparrevik J (2000) Effect of nutrient supplements on biodegradation of PAHs in a creosote contaminated soil. Chemosphere 56:174–188

    Google Scholar 

  • Bryselbout C, Henner P, Carsignol J, Lichtfouse E (2000) Polycyclic aromatic hydrocarbons in highway plants and soils. Evidence for a local distillation effect. Analusis 28:290–293

    Article  CAS  Google Scholar 

  • Budavari S, O’Neil JM, Smith A, Heckehman EP, Kinneary JF (1996) The Merck index, 12th edn. Merck Research Laboratory Division, Whitehouse Station, NJ, pp 115, 1094–1095, 1241

  • Cajthaml T, Erbanová P, Šašek V, Moeder M (2006) Breakdown products on metabolic pathway of degradation of benz[a]anthracene by a ligninolytic fungus. Chemosphere 64:560–564

    Article  CAS  Google Scholar 

  • Chi FH, Amy GL (2004) Transport of anthracene and benz(a)anthracene through iron-quartz and three aquifer materials in laboratory columns. Chemosphere 4:515–524

    Article  Google Scholar 

  • Cohen R, Persky L, Hadar Y (2002) Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus. Appl Microbiol Biotechnol. Retrieved August 26, 2004, from http://www.springerlink.com/app/home/content.asp

  • Conte P, Zena A, Pilidis G, Piccolo A (2000) Increased retention of polycyclic aromatic hydrocarbons in soils induced by soil treatment with humic substances. Environ Pollut 112:27–31

    Article  Google Scholar 

  • Croan SC (2000) Conversion of wood waste into value-added products by edible and medicinal Pleurotus (Fr.) P. Karst. Species (Agaricales s. I., Basidiomycetes). Int J Med Mushrooms 2:73–80

    Google Scholar 

  • de Abrantes R, de Assuncao JV, Pesquero CR (2004) Emission of polycyclic aromatic hydrocarbons from light-duty diesel vehicles exhaust. Atmos Environ 38:1631–1640

    Article  Google Scholar 

  • Delille D, Coulon F, Pelletier E (2004) Effects of temperature warming during a bioremediation study of natural and nutrient-amended hydrocarbon-contaminated sub-Antarctic soils. Cold Reg Sci Technol 40:61–70

    Article  Google Scholar 

  • Dodor DE, Hwang H-M, Ekunwe SIN (2004) Oxidation of anthracene and benzo_a_pyrene by immobilization laccase from Trametes versicolor. Enzyme Microb Technol 35:210–217

    Article  CAS  Google Scholar 

  • Dvorak MA, Oswald GA, Van Benthem MH, Gillispie GD (1997) On-the-fly fluorescence lifetime determination with total emission detection in HPLC. Anal Chem 9:3458–3464

    Article  Google Scholar 

  • Easton MDL, Luszniak D, Geest EV (2002) Preliminary examination of contaminant loadings in farmed salmon, wild salmon and commercial salmon feed. Chemosphere 46:1053–1074

    Article  CAS  Google Scholar 

  • Eggen T, Majcherczyk A (1998) Removal of polycyclic aromatic hydrocarbons (PAH) in contaminated soil by white rot fungus Pleurotus ostreatus. Int Biodeterior Biodegradation 41:111–117

    Article  CAS  Google Scholar 

  • Eibes G, Cajthaml T, Moreira MT, Feijoo G, Lema JM (2006) Enzymatic degradation of anthracene, dibenzothiophene and pyrene by manganese peroxidase in media containing acetone. Chemosphere 64:408–414

    Article  CAS  Google Scholar 

  • Gomez KL, Gomez AA (1984) Statistical procedures for agricultures research. Wiley, NY

    Google Scholar 

  • Inyang F, Ramesh A, Kopsombut P, Niaz MS, Hood DB, Nyanda AM, Archibong AE (2003) Disruption of testicular steroidogenesis and epididymal function by inhaled benzo(a)pyrene. Reprod Toxicol 17:527–537

    Article  CAS  Google Scholar 

  • IUPAC (1997) Gravimetric method. Compend Chem Terminol 62:2192

    Google Scholar 

  • Iwamoto T, Nasu M (2001) Current bioremediation practice and perspective. J Biosci Bioeng 92:1–8

    Article  CAS  Google Scholar 

  • Jacques RJS, Santos EC, Bento FM, Peralba MCR, Selbach PA, Sá ELS, Camargo FAO (2005) Anthracene biodegradation by Pseudomonas sp. isolated from a petrochemical sludge landfarming. Int Biodeg Biodet 56:143–156

    Article  CAS  Google Scholar 

  • Kamitsuji J, Honda Y, Watanabe T, Kuwahara M (2004) Mn2+ is dispensable for the production of active MnP2 by Pleurotus ostreatus. Biochem Biophys Res Commun 327:871–876

    Article  Google Scholar 

  • Karimi-Lotfabad S, Pickard M, Gray M (1996) Reactions of polynuclear aromatic hydrocarbons on soil. Environ Sci Technol 30:1145–1151

    Article  CAS  Google Scholar 

  • Kium JY, Li CP (2004) Enhanced solubilization and removal of naphthalene and phenanthrene using acetone, cyclohexane. J Chromatogr 54:177–190

    Google Scholar 

  • Klute A (ed) (1986) Methods of soil analysis: physical and mineralogical methods. American Society of Agronomy, Soil Science Society of America, Inc., Madison, WI

    Google Scholar 

  • Kordel W, Dassenakis M, Lintelmann J, Padberg S (1997) The importance of natural organic material for environmental processes in waters and soils. Pure Appl Chem 69:1571–1600

    Article  CAS  Google Scholar 

  • Kottler BD, White JC, Kelsey JW (2000) Influence of soil moisture on the sequestration of organic compounds in soil. Chemosphere 42:893–898

    Article  Google Scholar 

  • Krivobok S, Miriouchkine E, Seigle-Murandi F, Benoit-Guyod JL (1998) Biodegradation of anthracene by fungi. Chemosphere 37:523–530

    Article  CAS  Google Scholar 

  • Lavrovsky V (2004). Microencapsulated enzyme systems. Enhanced oil recovery and bioremediation. Paper presented at the 2004 Calgary youth science fair. Retrieved on August 18, 2004, from http://www.physics.uwo.ca/teamcana/2003/vladic_lavrovsky_report.pdf

  • Lentner M, Bishop T (1993) Experimental design and analysis, 2nd edn. Valley Book Company, Blacksburg, Virginia

    Google Scholar 

  • Mallinckrodt Baker, Inc. (2002). Solvents and solvent blends for LC/MS analysis. Retrieved 03/20/2004 from https://www.vwrsp.com/ literature/products/pdf/MallinckrodtBakerLC-MS_ Solvents_ and_ Solvent_ Blends_Flyer.pdf

  • Miller KP, Borgeest C, Greenfield C, Tomic D, Flaws JA (2004) In utero effects of chemicals on reproductive tissues in females. Toxicol Appl Pharmacol 198:111–131

    Article  CAS  Google Scholar 

  • Mulder H, Breure AM, Rulkens WH (2001) Prediction of complete bioremediation periods for PAH soil pollutants in different physical states by mechanistic models. Chemosphere 43:1085–1094

    Article  CAS  Google Scholar 

  • Mulvaney RL (1996) Nitrogen—inorganic forms. In: Sparks DL (ed) Methods of soil analysis: part 3—chemical methods. SSSA Book Ser No 5 SSSA and ASA, Madison, WI, pp 1123–1184

    Google Scholar 

  • Ping W, Ke-Zhao D, Ya-Xian Z, Yong Z (2008) A novel analytical approach for investigation of anthracene adsorption onto mangrove leaves. Talanta 76:1177–1182

    Article  Google Scholar 

  • Pizzul L, Sjögren A, Pilar M, Stenström C, Stenström J (2007) Degradation of polycyclic aromatic hydrocarbons in soil by a two-step sequential treatment. Biodegradation 18:607–616

    Article  CAS  Google Scholar 

  • Pozdnyakova NN, Rodakiewicz-Nowak J, Turkovskaya OV, Haber J (2006) Oxidative degradation of polyaromatic hydrocarbons catalyzed by blue laccase from Pleurotus ostreatus D1 in the presence of synthetic mediators. Enzyme Microb Technol 39:1242–1249

    Article  CAS  Google Scholar 

  • Raghavan PUM, Vivekanandan M (1999) Bioremediation of oil-spilled sites through seeding of naturally adapted Pseudomonas putida. Int Biodeterior Biodegradation 44:29–32

    Article  Google Scholar 

  • Ramsay JA, Hao L, Brown RS, Ramsay BA (2003) Naphthalene and anthracene mineralization linked to oxygen, nitrate, Fe(II) and sulphate reduction in a mixed microbial population. Biodegradation 14:321–329

    Article  CAS  Google Scholar 

  • Rodriguez E, Guillen NF, Martinez MJ (2004) Degradation of phenolic and non-phenolic aromatic pollutants by four Pleurotus species: role of laccase and versatile peroxidase. Soil Biol Chem 36:909–916

    Article  CAS  Google Scholar 

  • Schutzendubel A, Majcherczyk A, Johannes C, Huttermann A (1999) Degradation of fluorine, anthracene, phenanthrene, fluoranthene, and pyrene lacks connection to the production of extracellular enzymes by Pleurotus ostreatus and Bjerkandera adusta. Int Biodeterior Biodegradation 43:93–100

    Article  CAS  Google Scholar 

  • Sparks DL, Page AL, Helmke PA, Loeppert RH, Soltanpour PN, Tabatabai MA, Johnson CT, Sumner ME (1996) Methods of soil analysis. Part 3: Chemical methods. Soil Science Society of America, Inc., American Society of Agronomy, Inc., Madison, WI

    Google Scholar 

  • Tekere M, Read JS, Mattiasson B (2004) Polycyclic aromatic hydrocarbon in extracellular fluids and static batch cultures of selected sub-tropical white rot fungi. J Biotechnol 115:367–377

    Article  Google Scholar 

  • United States Department of Agriculture (1980) Soil survey of Stearns County, Minnesota. National Cooperative Soil Survey, USA

    Google Scholar 

  • United States Environmental Protection Agency (1996) Bioremediation of hazardous waste sites: Practical approaches to implementation. Retrieved on 15 Aug 2004, from http://www.epa.gov/ordntrnt/ORD/WebPubs/biorem/biohaz1.pdf

  • United States Environmental Protection Agency (2001) A citizen’s guide to bioremediation; the citizen’s guide series. Retrieved on 20 Sept 2004, from www.epa.gov/superfund/sites

  • United States Environmental Protection Agency (2003) Control of emissions of air pollution from non-road diesel engines and fuel. Propose rule. Retrieved on 15 Aug 2004, from http://www.epa.gov/otaq/url-fr/fr23my03p.pdf

  • United States Environmental Protection Agency (2004) Persistent organic pollutants: A global issue, a global response. Retrieved on 15 Aug 2004, from http://www.epa.gov/international/toxics/pop.pdf

  • Walworth J, Braddock J, Woolard C (2001) Nutrient and temperature interactions in bioremediation of cryic soils. Cold Reg Sci Technol 32:85–91

    Article  Google Scholar 

  • Weigand H, Totsche K, Kögel-Knabner I, Annweiler E, Richnow H, Michaelis W (2002) Fate of anthracene in contaminated soil: transport and biochemical transformation under unsaturated flow conditions. Eur J Soil Sci 53:71–81

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Civil and Environmental Engineering, University of North Carolina, Charlotte, NC, USA

    Humphrey O. Zebulun & Hilary Inyang

  2. School of Agriculture & Environmental Sciences, North Carolina A & T University, Greensboro, NC, USA

    Omoanghe S. Isikhuemhen

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  1. Humphrey O. Zebulun
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  2. Omoanghe S. Isikhuemhen
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  3. Hilary Inyang
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Correspondence to Humphrey O. Zebulun.

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Zebulun, H.O., Isikhuemhen, O.S. & Inyang, H. Decontamination of anthracene-polluted soil through white rot fungus-induced biodegradation. Environmentalist 31, 11–19 (2011). https://doi.org/10.1007/s10669-010-9284-7

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